“…Traditionally, nanoscale investigation of the internal structure within nanofibers has been possible using various techniques including soft X-ray and electron microscopies, which are limited to investigations of thin samples (Shenhar & Rotello, 2003;. For example, in transmission electron microscopy, sample preparation and mechanical slicing procedures are essential for thick samples, and scanning electron microscopy only enables surface or cross-section microstructures to be observed (McIntosh et al, 2005;Wei et al, 2017). Typically, nanotomography is the commonly used technique for the three-dimensional (3D) imaging-based scanning transmission soft X-ray microscope (STXM), which is still a challenge for thick, large size samples due to high absorption for rotation at large angles (Johansson et al, 2006;Schmid et al, 2014).…”