Preparation, characterization, in vitro drug release, and cellular interactions of tailored paclitaxel releasing polyethylene oxide films for drug-coated balloons

Anderson, Jordan A.; Lamichhane, Sujan; Remund, Tyler; Kelly, Patrick; Mani, Gopinath

doi:10.1016/j.actbio.2015.09.036

Cited by 32 publications

(36 citation statements)

References 32 publications

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“…The viability, proliferation, morphology, and phenotype of ECs and SMCs were studied as we previously reported . The viability and proliferation of ECs and SMCs were measured using resazurin fluorometric assay (Biotium, Hayward, CA) .…”

Section: Methodsmentioning

confidence: 99%

“…The phenotype of ECs and SMCs was examined using the immunofluorescence microscopy technique . Platelet EC adhesion molecule (PECAM‐1) antibody, vascular cell adhesion molecule (VCAM‐1) antibody, intercellular adhesion molecule (ICAM‐1) antibody, goat anti‐rabbit IgG‐FITC, smooth muscle α‐actin antibody, goat anti‐mouse IgG‐FITC, goat serum, and Triton X‐100 were all purchased from Santa Cruz Biotechnology (Santa Cruz, CA).…”

Section: Methodsmentioning

confidence: 99%

“…Thus, stained cells were imaged using a spectral confocal microscope (Olympus IX83 FV1200, Olympus Lifescience). The phenotype of ECs and SMCs were quantitatively measured using the Image J software as we previously described . Briefly, the fluorescence intensity of green colored staining in the cells was measured from the immunofluorescence microscopy images.…”

Section: Methodsmentioning

confidence: 99%

“…The viability, proliferation, morphology, and phenotype of ECs and SMCs were studied as we previously reported. [19][20][21][22] The viability and proliferation of ECs and SMCs were measured using resazurin fluorometric assay (Biotium, Hayward, CA). [19][20][21][22] For the morphology study, the ECs and SMCs were stained with fluorescein diacetate (FDA) and imaged using an Axiovert 200 M fluorescence microscope (Carl Zeiss, Thornwood, NY).…”

Section: Surface Characterizationsmentioning

confidence: 99%

“…[19][20][21][22] The viability and proliferation of ECs and SMCs were measured using resazurin fluorometric assay (Biotium, Hayward, CA). [19][20][21][22] For the morphology study, the ECs and SMCs were stained with fluorescein diacetate (FDA) and imaged using an Axiovert 200 M fluorescence microscope (Carl Zeiss, Thornwood, NY). [19][20][21][22] The morphology of ECs and SMCs was also quantitatively determined by measuring the cell aspect ratio and the cell area using the Image J software as we reported previously.…”

Section: Surface Characterizationsmentioning

confidence: 99%

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Responses of endothelial cells, smooth muscle cells, and platelets dependent on the surface topography of polytetrafluoroethylene

Lamichhane

Anderson

Remund

et al. 2016

J Biomedical Materials Res

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In this study, the effect of different structures (flat, expanded, and electrospun) of polytetrafluoroethylene (PTFE) on the interactions of endothelial cells (ECs), smooth muscle cells (SMCs), and platelets was investigated. In addition, the mechanisms that govern the interactions between ECs, SMCs, and platelets with different structures of PTFE were discussed. The surface characterizations showed that the different structures of PTFE have the same surface chemistry, similar surface wettability and zeta potential, but uniquely different surface topography. The viability, proliferation, morphology, and phenotype of ECs and SMCs interacted with different structures of PTFE were investigated. Expanded PTFE (ePTFE) provided a relatively better surface for the growth of ECs. In case of SMC interactions, although all the different structures of PTFE inhibited SMC growth, a maximum inhibitory effect was observed for ePTFE. In case of platelet interactions, the electrospun PTFE provided a better surface for preventing the adhesion and activation of platelets. Thus, this study demonstrated that the responses of ECs, SMCs, and platelets strongly dependent on the surface topography of the PTFE. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2291-2304, 2016.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Surface Characterizationsmentioning

confidence: 99%

Section: Surface Characterizationsmentioning

confidence: 99%

See 3 more Smart Citations

Responses of endothelial cells, smooth muscle cells, and platelets dependent on the surface topography of polytetrafluoroethylene

Lamichhane

Anderson

Remund

et al. 2016

J Biomedical Materials Res

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Understudied factors in drug‐coated balloon design and evaluation: A biophysical perspective

Shazly

Torres

Secemsky

et al. 2022

Bioengineering & Transla Med

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Drug‐coated balloon (DCB) percutaneous interventional therapy allows for durable reopening of the narrowed lumen via physical tissue expansion and local anti‐restenosis drug delivery, providing an alternative to traditional uncoated balloons or a permanent indwelling implant such as a conventional metallic drug‐eluting stent. While DCB‐based treatment of peripheral arterial disease (PAD) has been incorporated into clinical guidelines, DCB use has been recently curtailed due to reports that showed evidence of increased mortality risk in patients treated with paclitaxel (PTX)‐coated balloons. Given the United States Food and Drug Administration's 2019 consequent warning regarding PTX‐eluting DCBs and the subsequent marked reduction in clinical DCB use, there is now a critical need to better understand the compositional and mechanical factors underlying DCB efficacy and safety. Most work to date on DCB refinement has focused on designing both the enabling balloon catheter and alternate coatings composed of various drugs and excipients, followed by device evaluation in preclinical and clinical studies. We contend that improvement in DCB performance will require a better understanding of the biophysical factors operative during and following balloon deployment, and moreover that the elaboration and demonstrated control of these factors are needed to address current concerns with DCB use. This article provides a perspective on the biophysical interactions that govern DCB performance and offers new design strategies for the development of next‐generation DCB devices.

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Development of an automated micropipette coating method for drug‐coated balloons

et al. 2020

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Drug-coated balloons (DCBs) are a recent technology developed to treat peripheral artery disease (PAD). Along with a suitable formulation of antiproliferative drug and excipient, coating method is an important aspect of a DCB as these factors affect coating characteristics and drug delivery to the treatment site. The multiple release tailored medical devices DCB (MR-TMD-DCB), designed to achieve multiple inflations to treat complex PAD, contains paclitaxel (PAT) as the antiproliferative drug and polyethylene oxide (PEO) as the excipient. In our previous studies, the MR-TMD-DCB was coated using a manual dip coating method. In this study, an automated micropipette coating method was developed using a modified spray coating instrument to coat the MR-TMD-DCB. First, the coating formulation and strategy was optimized. A drug formulation of 16 wt% PAT and 4% wt/vol PEO, a polymer formulation of 2.5% wt/vol PEO, and a total of two drug layers produced a mostly uniform and thin coating with no defects and acceptable drug load. The balloon also had optimal drug uptake in arterial tissue in an in vitro flow model. Next, the reproducibility of the coating strategy was improved by optimizing the instrument parameters. The optimized instrument parameters (translational speed = 0.150 in/s, revolution rate = 100 rpm, flow rate = 0.6 ml/min) resulted in improved reproducibility of the drug load and similar coating properties as the DCB. This study demonstrated the ability to automate the micropipette process to obtain a balloon with optimal coating properties and drug tissue uptake.

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Preparation, characterization, in vitro drug release, and cellular interactions of tailored paclitaxel releasing polyethylene oxide films for drug-coated balloons

Cited by 32 publications

References 32 publications

Responses of endothelial cells, smooth muscle cells, and platelets dependent on the surface topography of polytetrafluoroethylene

Responses of endothelial cells, smooth muscle cells, and platelets dependent on the surface topography of polytetrafluoroethylene

Understudied factors in drug‐coated balloon design and evaluation: A biophysical perspective

Development of an automated micropipette coating method for drug‐coated balloons

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