Preparation, characterization, transfection efficiency, and cytotoxicity of liposomes containing oligoamine-modified cholesterols as nanocarriers to Neuro2A cells

Malaekeh‐Nikouei, Bizhan; Malaekeh-Nikouei, Mohammad; Oskuee, Reza Kazemi; Ramezani, Mohammad

doi:10.1016/j.nano.2009.02.001

Cited by 16 publications

(12 citation statements)

References 24 publications

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“…It was demonstrated that the cationic liposomes formed by lipid 2а had the highest ζ-potential in comparison with liposomes formed by lipids 1a,b, 2b,с and 3а, and exhibited the highest TE with respect to HeLa, COS-7, and NIH 3T3 cells (Takeuchi et al, 1996). However the dependence of the structure of the lipid, ξ-potential and the TE was not always obvious (Kearns et al, 2008;Malaekeh-Nikouei et al, 2009). …”

Section: Influence Of Surface Chargementioning

confidence: 99%

See 1 more Smart Citation

Non-Viral Gene Delivery Systems Based on Cholesterol Cationic Lipids: Structure-Activity Relationships

Маслов¹,

Зенкова²

2011

Non-Viral Gene Therapy

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Section: Influence Of Surface Chargementioning

confidence: 99%

“…A number of researchers have argued that either size of the formed lipoplexes is not associated with TE, or that TE is not affected by initial lipoplex size (Han et al, 2008;Kearns et al, 2008;Malaekeh-Nikouei et al, 2009). Some researchers posit that large lipoplexes possess higher TE in comparison to the smaller ones.…”

Section: Influence Of Sizementioning

confidence: 99%

Non-Viral Gene Delivery Systems Based on Cholesterol Cationic Lipids: Structure-Activity Relationships

Маслов¹,

Зенкова²

2011

Non-Viral Gene Therapy

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“…The acute toxicity and chronic toxicity trials of DOX-Hydrochloride Nanoliposome (DHNP) prepared by ethanol injection-pH gradient method possesses the advantage of uniform distribution size, high encapsulation efficiency, big drug loading rate, and its toxicity is lower than free DOX [93].…”

Section: Liposomal Nanomedicine: Preclinical and Clinical Studiesmentioning

confidence: 99%

“…The best transfection efficiency and the noncytotoxicity of the prepared lipoplexes in Neuro2A cells was tested using pDNA encoding Renilla luciferase. These nanocarriers are able to transfer the DNA into Neuro2A cells with low or no toxicity [93].…”

Section: Risks and Safety Issuesmentioning

confidence: 99%

Multidynamic Liposomes in Nanomedicine: Technology, Biology, Applications, and Disease Targeting

Ghosh

Ansar²

2014

Nanoparticles' Promises and Risks

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Biocompatible and biodegradable liposome nanomedicine are finding large-scale application in delivery of drugs, RNA, antisense oligonucleotides, DNA, plasmids carrying gene, antioxidants to targeted sites for diagnosis, and treatment at cellular and molecular level of disease both in human and livestock.Conventional drug delivery system suffers from the disadvantage of narrow therapeutic index, poor serum solubility, and nonspecific target effects. Circumventing this problem, liposome drug nanoparticles are designed with the advantage of enhanced permeability, retention effect, selective targeted delivery, stimuli-induced release that can evade the host defense system, cross the bloodbrain barrier, thus conferring, synergetic effects, small interfering RNA (siRNA) co-delivery, multimodality therapies, and oral delivery of therapeutics at disease sites. Liposomes with diverse range of modifications are being designed to generate improvised medicine delivery systems. Immuno-liposomes, linked with antibodies are promising tools for targeted drug delivery to specific cancer cells. In the recent times several anti-cancer agents in nanodelivery systems are under preclinical and clinical studies. Lipid-based liposomal delivery systems of self-amplifying RNA vaccines are showing promising effects.Globally research is being carried out in generating liposomes with higher compatibility within the biological system in targeting a wide array of diseases including cancer, overcoming multidrug resistance, inflammatory diseases, thrombosis, psoriasis, muscular dystrophy, spinal cord injury, neurologic diseases, diabetes, parasite induced diseases, and cardiovascular diseases.

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“…Novel gene delivery system was developed using liposomes composed of DOTAP and oligoamines-modified cholesterol. All of these liposomes showed the ability to transfer the DNA into Neuro2A cells [16].…”

Section: Introductionmentioning

confidence: 99%

Liposome-linear polyethyleneimine-DNA Nanocomplexes for Gene Delivery: Preparation, Characterization and In Vitro Transfection Activity

Motaharinia¹,

Ramezani²,

Malaekeh-Nikouei

et al. 2011

CNANO

Self Cite

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The aim of the present study was to improve transfection efficiency using different combinations of cationic liposomes, linear polyethylenimine and DNA. A novel gene delivery system (lipopolyplex) was developed by premixing cationic liposomes containing cholesterol or oligoamine modified cholesterol (derivative I-III) and linear polyethyleneimines (PEIs) following addition of plasmid at three different C/P ratios. The resultant complexes were characterized for their size, zeta potential and ability of DNA condensation. Luciferase reporter gene was used for determination of transfection efficiency in Neuro2A cells. Mean particle size of prepared complexes was less than 200 nm and they showed positive surface charge. The transfection efficiency of vectors was reduced by increasing in carrier concentration/plasmid DNA ratio (C/P ratio) while gene expression of cationic liposome or PEI was increased at higher C/P ratios. Complexes composed of PEI 2.5 or 250 kDa and liposome containing derivative I had the highest transfection activity. Furthermore, non-viral vectors described in this study showed low cytotoxicity. The results show that small and large molecular weight linear PEI in combination with liposome have little toxicity and may enhance transfection efficiency.

show abstract

Preparation, characterization, transfection efficiency, and cytotoxicity of liposomes containing oligoamine-modified cholesterols as nanocarriers to Neuro2A cells

Cited by 16 publications

References 24 publications

Non-Viral Gene Delivery Systems Based on Cholesterol Cationic Lipids: Structure-Activity Relationships

Non-Viral Gene Delivery Systems Based on Cholesterol Cationic Lipids: Structure-Activity Relationships

Multidynamic Liposomes in Nanomedicine: Technology, Biology, Applications, and Disease Targeting

Liposome-linear polyethyleneimine-DNA Nanocomplexes for Gene Delivery: Preparation, Characterization and In Vitro Transfection Activity

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