Preparation, characterizations and anti-pollutant activity of 7,3′,4′-trihydroxyisoflavone nanoparticles in particulate matter-induced HaCaT keratinocytes

Abstract

Background7,3′,4′-Trihydroxyisoflavone (734THI), a secondary metabolite derived from daidzein in soybean, possesses several biological activities, including antioxidant, skin whitening and anti-atopic dermatitis properties, but the poor aqueous solubility of 734THI has limited its application in medicine and cosmetic industry.MethodsThe aim of the present study was to improve the physicochemical properties of 734THI using planetary ball mill preparation under a solvent-free process to improve its solubility and anti-pollutant activity.Results734THI nanoparticle powder (734THIN) was successfully prepared by the planetary ball mill technique using polyvinylpyrrolidone K30 as the excipient. 734THIN effectively increased the aqueous solubility and cellular uptake of 734THI by improving its physicochemical properties, including particle size reduction, crystalline–amorphous transformation and intermolecular hydrogen bonding with polyvinylpyrrolidone K30. In addition, 734THIN inhibited the overexpression of COX-2 and MMP-9 by downregulating MAPK pathway signaling in particulate matter-exposed HaCaT keratinocytes, while raw 734THI in PBS with low aqueous solubility did not show any anti-inflammatory or antiaging activity.Conclusion734THIN may be used as an additive in anti-pollutant skin care products for preventing particulate matter-induced inflammation and aging in skin.

Glycofullerenes Inhibit Particulate Matter Induced Inflammation and Loss of Barrier Proteins in HaCaT Human Keratinocytes

“…al. [23] and it can be a reliable differentiation model to estimate the inflammatory and repair response, as well as a cell platform for the development of novel treatments and the investigation of disease mechanisms [5,[24][25][26][27][28]. In our The HaCaT cell line was first generated by Boukamp et.…”

“…[23] and it can be a reliable differentiation model to estimate the inflammatory and repair response, as well as a cell platform for the development of novel treatments and the investigation of disease mechanisms [5,[24][25][26][27][28]. In our previous study [27,29], we prepared water-soluble 7, 3 , 4 -trihydroxyisoflavone NPs by using the planetary ball mill method [27] and eupafolin NP delivery system [25,29] to investigate their individual antioxidant and anti-inflammatory activities in PM-induced skin inflammation [5,24,25,29]. The results of our previous study suggested that PM-induced skin cell inflammation regulated the AhR/p47 phox/NADPH oxidase pathway, and subsequently increased ERK1/2, p38/nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), and c-Jun N-terminal kinase (JNK)/Activator protein 1 (AP-1) levels [5].…”

LncRNA RP6‐65G23.1 accelerates proliferation and inhibits apoptosis via p‐ERK1/2/p‐AKT signaling pathway on keratinocytes

“…In this study, we explored the role of lncRNA-RP6-65G23.1 in the proliferation and apoptosis of HaCaT cell, an immortalized human keratinocytes cell line frequently used as a model for psoriasis studies in vitro. [19][20][21] Our results showed that RP6-65G23.1 was involved in keratinocyte proliferation by regulating the G1/S phase progression and expression of p-AKT/p-ERK1/2 signaling proteins, in addition, RP6-65G23.1 interacted with Bcl2 and Bcl-xl to involve in keratinocyte apoptosis.…”

Effects of Microwave-Assisted Opuntia humifusa Extract in Inhibiting the Impacts of Particulate Matter on Human Keratinocyte Skin Cell

“…The PM-induced HaCaT cells model was built as described in Huang et al [30]. Briefly, PM was suspended in PBS and then sonicated for 30 min in a sonication bath.…”