Preparation, deproteinization, characterization, and antioxidant activity of polysaccharide from Medemia argun fruit

Mohammed, Jalaleldeen Khaleel; Mahdi, Amer Ali; Ahmed, Mohamed Ismael; Ma, Mengjiao; Wang, Hongxin

doi:10.1016/j.ijbiomac.2019.11.050

Cited by 85 publications

(30 citation statements)

References 29 publications

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“…The degree of substitution of phosphorylated polysaccharides was determined by iron phosphomolybdate method (Chen, 2019). The degree of substitution of acetylated polysaccharide is titrated with hydrochloric acid of the same concentration to the polysaccharide alkali solution added with phenolphthalein (Mohammed et al., 2019). The degree of substitution of carboxymethylated polysaccharide was titrated with calibrated hydrochloric acid solution.…”

Section: Structural Characterization Of Plant Polysaccharidesmentioning

confidence: 99%

Extraction, separation, modification, structural characterization, and antioxidant activity of plant polysaccharides

Huang

2020

Chem Biol Drug Des

132

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Polysaccharide is an important macromolecule with biological activity, and its biological activity is closely related to its structure. The extraction methods and modification methods of polysaccharides directly affect the structure of polysaccharides and then affect the biological activities of polysaccharides. In this paper, the extraction, separation, and modification of polysaccharides and their advantages and disadvantages were reviewed, and the research methods of structure characterization and antioxidant activity were discussed. This not only provides ideas for optimizing the extraction, separation, and modification methods of plant polysaccharides, but also provides the basic theoretical basis for the structural analysis and antioxidant activity research of plant polysaccharides.

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Section: Structural Characterization Of Plant Polysaccharidesmentioning

confidence: 99%

Extraction, separation, modification, structural characterization, and antioxidant activity of plant polysaccharides

Huang

2020

Chem Biol Drug Des

132

View full text Add to dashboard Cite

show abstract

“…The signal at 1398 cm –1 was contributed by the bending vibrations of C–H (Li et al., 2019; Xiang et al., 2019). Moreover, the pyranoside ring absorption band was observed at 878 cm –1 (C–H ring vibration) (Nejatzadeh‐Barandozi & Enferadi, 2012) and indicated a β‐glycosidic linkage (Mohammed et al., 2020).…”

Section: Resultsmentioning

confidence: 99%

Ultrasonic extraction, structural characterization, and antioxidant activity of oligosaccharides from red yeast rice

Liu

Xie

et al. 2021

Food Science & Nutrition

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Red yeast rice is consumed as a medicinal food to lower blood lipids. Besides, it is used to color food, make wine, etc. In this study, water‐soluble oligosaccharides in red yeast rice were extracted by ultrasonic‐assisted extraction method. The parameters to extract oligosaccharides from red yeast rice were optimized by the Box‐Behnken design under the following optimal extraction conditions: extraction temperature, 60°C; extraction time, 97 min; and liquid/material ratio, 25 ml/g. The structure and the antioxidant activity of the new oligosaccharide were preliminarily investigated. Total carbohydrates extracted from red yeast rice with 80% ethanol–water solution (v/v) were first removed from pigments using D101 macroporous adsorption resin. The total sugar contents were then purified by DE52 resins and Sephadex G‐25 resins to obtain red yeast rice oligosaccharides, coded as RYRO1. Structural characterization experiments indicated that RYRO1 is an oligosaccharide with a weight average molecular weight of 874 Da and a theoretical degree of polymerization of 4.86. RYRO1 is composed of mannose, glucosamine, glucose, and galactose with a molar ratio of 0.248:0.019:1:0.026. The ABTS, DPPH, and hydroxyl free radical scavenging assays showed antioxidant nature of RYRO1.

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“…The UV spectrum scanning of RPs-1, RPs-2, RPs-3, RPs-4, and RPs-5 was performed within the wavelength range of 200-600 nm, and the results are depicted in Figure 1a. It can be observed from the figure that there was no significant absorption peak at 260-280 nm of the samples, indicating the absence of nucleic acid and protein in the sample (Mohammed et al, 2020). Meanwhile, a single absorption peak was observed at 190-200 nm, indicating that RPs-1, RPs-2, RPs-3, RPs-4, and RPs-5 owned the characteristics of polysaccharides.…”

Section: Uv Spectrum Analysismentioning

confidence: 93%

“…RPs-1, RPs-2, RPs-3, RPs-4, and RPs-5 were weighed and prepared into 0.5 mg/ml polysaccharide solution. The ultraviolet spectrum characteristics of RPs were analyzed by full wavelength scanning in the range 200-600 nm (Mohammed et al, 2020).…”

Section: Uv Spectrum Analysismentioning

confidence: 99%

Antioxidant activity and rheological properties of the polysaccharides isolated from Ribes stenocarpum maxim with different extraction methods

Qiao

Cai

et al. 2021

J. Food Process. Preserv.

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The purpose of the present study is to illustrate the effects of extraction methods on the antioxidant activity and rheological properties of polysaccharides isolated from Ribes stenocarpum Maxim (RPs). RPs were extracted by ultrasonic-assisted extraction (RPs-1), alkaline extraction (RPs-2), acid extraction (RPs-3), microwaveassisted extraction (RPs-4), and three-phase separation (RPs-5). The results showed that RPs-1, RPs-2, RPs-3, RPs-4, and RPs-5 are acidic heteropolysaccharides with glucose, arabinose, and galacturonic acid as the main chain units and absence of triple-helix structure. Of them, RPs-3 possess the highest uronic acid content, best thermal stability, and strongest DPPH, ABTS, and hydroxyl radical scavenging abilities. Rheological properties showed that the above-mentioned polysaccharides are non-Newtonian fluids and own the similar shear thinning properties. RPs-3 represent the highest apparent viscosity and better stability in the over-acid or overalkali environment. The above results indicated that the acid extraction could serve as the most favorable extraction method for RPs. Novelty impact statement: A novel polysaccharide was isolated from Ribes stenocarpumMaxim by acid extraction, showing a stronger antioxidant activity and excellent rheological properties, which has a good application prospect in food processing.

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Preparation, deproteinization, characterization, and antioxidant activity of polysaccharide from Medemia argun fruit

Cited by 85 publications

References 29 publications

Extraction, separation, modification, structural characterization, and antioxidant activity of plant polysaccharides

Extraction, separation, modification, structural characterization, and antioxidant activity of plant polysaccharides

Ultrasonic extraction, structural characterization, and antioxidant activity of oligosaccharides from red yeast rice

Antioxidant activity and rheological properties of the polysaccharides isolated from Ribes stenocarpum maxim with different extraction methods

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