Preparation, identification, and activity evaluation of ten antioxidant peptides from protein hydrolysate of swim bladders of miiuy croaker (Miichthys miiuy)

et al. 2019

J Food Biochem

Self Cite

In this work, alcalase and flavorzyme were chosen as the hydrolase for preparing high Fischer ratio oligopeptides from Antarctic krill (Euphausia superba) (HFP) using sequential enzyme hydrolysis process, and their hydrolysis conditions were optimized using single factor experiment. According to the Fischer ratio, granular activated carbon of XHJ‐200 (200 mesh) showed the best way to remove aromatic amino acids and its optimal parameters were pH value of 6.0, adsorption time of 2.5 hr, temperature of 25°C, and solid–liquid ratio of 1:20. The Fischer ratio and average molecular weight of HFP were 21.12 (>20) and 779.9 Da, respectively. In addition, the peptide profile of HFP was established using RP‐HPLC and 23 oligopeptides isolated from HFP including 6 dipeptides, 9 tripeptides, 3 tetrapeptides, and 5 pentapeptides were identified using protein amino acid sequence analyzer and mass spectrum. Furthermore, HFP exhibited high radical scavenging activity, reducing power, and lipid peroxidation inhibition capability. Practical applications High Fischer ratio oligopeptides (HFO) is a kind of small peptide mixture with the mole ratio of branched‐chain amino acids (BCAA) to aromatic amino acids (AAA) higher than 20, which has drawn a great attention due to its great potential in clinical nutrition approaches for the treatment of liver disease when amino acid composition is out of proportion characterized by low levels of BCAA and high levels of AAA in the systemic blood. Antarctic krill is regarded as the largest animal protein resource for various food and pharmaceutical products. However, there is no report on the preparation of HFO of Antarctic krill (HFP). Therefore, the aim of the present study was to investigate the preparation process, peptide profiles and in vitro antioxidant activity of HFP. This study will potentially enhance the value‐added utilization of Antarctic krill by making it an important raw material in the health‐promoting functional products.

Section: Resultsmentioning

confidence: 99%

High Fischer ratio oligopeptides determination from Antartic krill: Preparation, peptides profiles, and in vitro antioxidant activity

Chen

et al. 2019

J Food Biochem

Self Cite

“…Figure 3B indicated that DPPH• and HO• scavenging activities of MUA-4-B were 86.39% ± 4.32% and 80.56% ± 3.55% at the concentration of 5.0 mg protein/mL, which were significantly higher than those of MUA-4 (DPPH• 76.64% ± 3.43%; HO• 69.39% ± 3.58%) and MUA-4-A (DPPH• 56.38% ± 3.62%; HO• 55.26% ± 4.39%) (p < 0.05). Gel filtration chromatography is a frequently-used prepared technique according to the MW of the separated substances and is applied to separate peptides from protein hydrolysates and their fractions, such as Spanish mackerel [30], miiuy croaker [10,20], ark shell [19], and blue-spotted stingray [22]. Therefore, fraction MUA-4-B was selected for the following isolation process.…”

Section: Gel Filtration Chromatography Of Mua-4mentioning

confidence: 99%

“…The EC 50 values of MMP-4, MMP-7, and MMP-12 were 0.39, 0.62, and 0.51 mg/mL, respectively, but their activities were still lower than that of the positive control of GSH at the same concentration. The EC 50 value of MMP-4 was lower than those of most APs from protein hydrolysates of red stingray cartilages (VPR: 4.61 mg/mL, IEPH: 1.90 mg/mL, LEEEE: 3.69 mg/mL, and IEEEQ: 4.01 mg/mL) [6], loach (PSYV: 17.0 mg/mL) [35], salmon pectoral fin (TTANIEDRR: 2.50 mg/mL) [36], Spanish mackerel skins (PFGPD: 0.80 mg/mL, PYGAKG: 3.02 mg/mL, and YGPM: 0.72 mg/mL) [37], skipjack tuna bone (GADIVA: 0.57 mg/mL) [23], and miiuy croaker swim bladders (FPYLRH: 0.51 mg/mL; GIEWA: 0.78 mg/mL; YLPYA: 3.63 mg/mL; VPDDD: 2.87 mg/mL) [20]. Therefore, the present result suggested that three isolated peptides (MMP-4, MMP-7, and MMP-12), especially MMP-4 could contribute an electron or hydrogen radical to suppress the DPPH• reaction.…”

Section: Dpph Scavenging Activitymentioning

confidence: 99%

“…In addition, APs from seafood and their processing by-products such as tuna, cuttlefish, sardinelle, bluefin leatherjacket, tilapia, and shrimp, also have been studied and showed potent antioxidant activities including radical scavenging activity, reducing power, and inhibition ability on lipid and protein oxidation [5,19]. Zhao et al reported that FPYLRH and GIEWA from protein hydrolysate of miiuy croaker swim bladders could strongly scavenge radicals and effectively inhibit lipid peroxidation [20]. PIIVYWK and FSVVPSPK from Mytilus edulis hydrolysates exhibited strong 2,2-diphenyl-1-picrylhydrazyl radical (DPPH•) scavenging and ORAC activities, and increased cell viabilities in H 2 O 2 -induced hepatotoxicity through upregulating heme oxygenase-1 (HO-1) level under normal and oxidative stress conditions in cultured hepatocytes [21].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Antioxidant Peptides from the Protein Hydrolysate of Monkfish (Lophius litulon) Muscle: Purification, Identification, and Cytoprotective Function on HepG2 Cells Damage by H2O2

Wang

et al. 2020

Marine Drugs

Self Cite

In the work, defatted muscle proteins of monkfish (Lophius litulon) were separately hydrolyzed by pepsin, trypsin, and in vitro gastrointestinal (GI) digestion methods, and antioxidant peptides were isolated from proteins hydrolysate of monkfish muscle using ultrafiltration and chromatography processes. The antioxidant activities of isolated peptides were evaluated using radical scavenging and lipid peroxidation assays and H2O2-induced model of HepG2 cells. In which, the cell viability, reactive oxygen species (ROS) content, and antioxidant enzymes and malondialdehyde (MDA) levels were measured for evaluating the protective extent on HepG2 cells damaged by H2O2. The results indicated that the hydrolysate (MPTH) prepared using in vitro GI digestion method showed the highest degree of hydrolysis (27.24 ± 1.57%) and scavenging activity on a 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical (44.54 ± 3.12%) and hydroxyl radical (41.32 ± 2.73%) at the concentration of 5 mg protein/mL among the three hydrolysates. Subsequently, thirteen antioxidant peptides (MMP-1 to MMP-13) were isolated from MPTH. According to their DPPH radical and hydroxyl radical scavenging activity, three peptides with the highest antioxidant activity were selected and identified as EDIVCW (MMP-4), MEPVW (MMP-7), and YWDAW (MMP-12) with molecular weights of 763.82, 660.75, and 739.75 Da, respectively. EDIVCW, MEPVW, and YWDAW showed high scavenging activities on DPPH radical (EC50 0.39, 0.62, and 0.51 mg/mL, respectively), hydroxyl radical (EC50 0.61, 0.38, and 0.32 mg/mL, respectively), and superoxide anion radical (EC50 0.76, 0.94, 0.48 mg/mL, respectively). EDIVCW and YWDAW showed equivalent inhibiting ability on lipid peroxidation with glutathione in the linoleic acid model system. Moreover, EDIVCW, MEPVW, and YWDAW had no cytotoxicity to HepG2 cells at the concentration of 100.0 µM and could concentration-dependently protect HepG2 cells from H2O2-induced oxidative damage through decreasing the levels of reactive oxygen species (ROS) and MDA and activating intracellular antioxidant enzymes of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px). These present results indicated that the protein hydrolysate and isolated antioxidant peptides from monkfish muscle, especially YWDAW could serve as powerful antioxidants applied in the treatment of some liver diseases and healthcare products associated with oxidative stress.

“…Nowadays, a variety of antioxidant peptides have been identified from meat muscles, animals, vegetables, fruits, plant protein, and other foods (Gao et al, 2019;Wu et al, 2018;Yuan et al, 2018;Zhang & Mu, 2017;Zhang, Gao, et al, 2018;Zhao, Luo, et al, 2018;Zhu, Zhang, Zhou, & Xu, 2016). Peptide GWWW isolated from myoglobin showed a strong antioxidant activity against peroxyl radicals, and they suggested that amino acid side chains can be a base to design peptides with activities against some special target ROS and RNS (Karadag, Ozcelik, & Saner, 2009).…”

mentioning

confidence: 99%

Analysis of antioxidant effect of two tripeptides isolated from fermented grains (Jiupei) and the antioxidative interaction with 4‐methylguaiacol, 4‐ethylguaiacol, and vanillin

Jiang

Food Science & Nutrition

Luo

et al. 2019

Jiupei (fermented grains) is the raw material for Baijiu distillation. The antioxidant activities of peptides Val‐Asn‐Pro (VNP) and Tyr‐Gly‐Asp (YGD) identified from Jiupei were evaluated according to in vitro chemical assays (e.g., 2,2′‐azino‐bis (3‐ethylbenzothiazoline‐6‐sulfonic acid) diammonium salt, 1,1‐diphenyl‐2‐picrylhydrazyl, and oxygen radical absorbance capacity) and 2,2′‐azobis (2‐methylpropionamide)‐dihydrochloride‐activated HepG2 cell model. The interaction on antioxidant activities between peptides (VNP and YGD) and three functional phenols (4‐methylguaiacol, 4‐ethylguaiacol, and vanillin) which were found in Baijiu was also measured. On the basis of the results, two peptides exhibited strong antioxidant ability in oxygen radical absorbance capacity (ORAC) assay. Furthermore, they suppressed the generation of reactive oxygen species. The intracellular antioxidant enzymatic system and nonenzymatic system were also regulated by VNP and YGD. In addition, it was confirmed that partial auxo‐action between peptides and phenols appeared mostly in chemical assays. The findings above might indicate that VNP and YGD are potent natural antioxidants in Jiupei even in Baijiu through distillation process and lay the foundation for illustrating the interactions among different functional substances in Baijiu.