Preparation of submicrogram nitrogen samples for isotope analysis by the GS1 emission spectrometer

Martin, Francis; Maudinas, Bernard; Chemardin, Michel; Gadal, Pierre

doi:10.1016/0020-708x(81)90052-1

Cited by 16 publications

(5 citation statements)

References 9 publications

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“…Soil samples were collected from 0 to 20 cm depths from all main and microplots. Soil samples were air-dried and ground to pass through a 100-mesh screen for total N and 15 N isotope analysis according to the method of Martin et al (1981). Ten representative hills of the plants were sampled and separated into leaf blades and root.…”

Section: Samplings and Analysesmentioning

confidence: 99%

Effect of N Management on Root Yield and N Uptake of Radishes in Southern China

Weiling

Yuan

Deng

et al. 2015

horts

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Efficient nitrogen (N) fertilizer management is crucial for ensuring the maximum economic yield and reducing the risk of environmental pollution. The objective of this study was to determine the effect of N fertilizer management on root yield and N uptake of radish in southern China by using 15N isotope tracing. A 2-year field experiment was conducted with three N rates (0, 60, and 120 kg N/ha) and two different application proportions, viz, A [50% at basal, 20% at 15 days after seeding (DAS), 30% at 30 DAS] and B (30% at basal, 20% at 15 DAS, 50% at 30 DAS) for each N rate, which were expressed as N0, N60A, N60B, N120A, and N120B, respectively. The results showed that root yields were significantly increased with N rates increasing from 0 to 120 kg N/ha. The root yields for N120A and N120B were 67.60 t·ha−1 and 72.50 t·ha−1 at harvest, 64.07% and 66.67% higher than those for the treatments of N60A and N60B, respectively. Mean radish recovery of N fertilizer ranged from 25.90% at N120A to 32.60% at N60B, and N fertilizer residual rate in the soil ranged from 11.50% at N120A to 14.90% at N60B. About 17.50% to 35.70% of total uptake of 15N derived from basal fertilizer was absorbed at seeding stage. However, 61.87% to 80.18% of total uptake of 15N derived from topdressing fertilizer absorbed at root expanding stage. Therefore, appropriate nitrogen application with increasing topdressing nitrogen amount could increase root yield of radish and the nitrogen recovery efficiency. Nitrogen fertilizer application recommended was 120 kg N/ha with 30% for basal, 20% for 15 DAS and 50% for 30 DAS in this study.

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Section: Samplings and Analysesmentioning

confidence: 99%

Effect of N Management on Root Yield and N Uptake of Radishes in Southern China

Weiling

Yuan

Deng

et al. 2015

horts

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“…Ci. Solo, Viçosa, 21:387-392, 1997 após a transformação do nitrogênio amoniacal em N 2 pelo método de DUMAS (Fiedler & Proksch, 1975;Martin et al, 1981).…”

Section: Methodsunclassified

Mineralização do nitrogênio incorporado como material vegetal em três solos da Amazônia Central

Alfaia

1997

Rev. Bras. Ciênc. Solo

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RESUMOA mineralização do N proveniente do resíduo de feijão-caupi marcado com 15 N foi estudada em condições de laboratório de setembro a dezembro de 1992. O material vegetal foi incorporado em amostras dos três principais solos da Amazônia Central: dois de terra firme, classificados como latossolo amarelo e podzólico vermelhoamarelo, e um de várzea, classificado como glei pouco húmico (GP). As variações nos teores e na forma de N mineral provenientes do resíduo de caupi foram relacionadas com as características químicas dos solos estudados. No latossolo e no podzólico, a incorporação do resíduo de caupi aumentou significativamente a mineralização do N, sendo a forma amoniacal predominante, enquanto, no GP, a forma nítrica preponderou. Nos solos de terra firme, a incorporação do resíduo de caupi aumentou a mineralização do N orgânico do solo, indicando a ocorrência do efeito "priming". Após 60 dias de incubação, cerca de 30 (podzólico) a 40% (latossolo) do N proveniente do caupi foi mineralizado nos solos de terra firme, enquanto no de várzea somente 18% foi mineralizado nesse período. Tais resultados mostram o potencial que essa leguminosa apresenta como fornecedora de N para as plantas nos solos de terra firme da Amazônia Central.Termos de indexação: 15 N, caupi, Vigna unguiculata L, mineralização, efeito "priming", latossolo, podzólico, glei pouco húmico, Amazônia Central.

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“…It is similar in concept to the reabsorption system of Yoneyama and Kumazawa (1974) in order to obtain NH4CI for colorimetric determin,ation of N and for isotope analysis. The atom% ^'N was determined by emission spectrometry using a SOPRA GSl '^N-analyser (Martin et al 1981).…”

Section: Methodsmentioning

confidence: 99%

Nitrate assimilation and nitrogen circulation in Austrian pine

Martin

Chemardin

Gadal

1981

Physiologia Plantarum

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P. 1981. Nitrate assimilation and nitrogen circulation in Austrian pine. -Physiol. Plant. 53: 105-110.Nitrate uptake, reduction and translocation were examined in 5-week-oId Austrian pines (Pintts nigra Arnold var. nigricans Host.) during exposure to 5 mJW NaNOj. The rate of nitrate uptake was line,ar during the 7 h light period. '^N-NOJ was detected in all parts of the pine except in the needles. By the 7th hour, 43% of the absorbed nitrate had been reduced, and this increased to 64% by the 24th hour. The noajor part of the total reduction occurred in the roots at this growth stage. Accumulation of ^^N in reduced soluble and insoluble fractions was more prevalent m roots than in shoots. In the needles, the translocated nitrogen was mainly incorporated into the insoluble fraction. It is likely that most of the nitrogen from nitrate was transported from the roots to the aerial org,ans as organic nitrogen; however part of the upward nitrogen flus took place as nitrate ions. An experiment in which an exposure for 24 h to 5 mM Na"'NO3 was followed by 13 days exposure to Na^NOj (pulse chase experiment) revealed a half time of about 1 day for depletion of root nitrate. A large p,art of this depletion was due to the loss of "N-NO3 to the nutrient solution. The remaining pool of "N-nitrate was partitioned between a metabolically inactive and an active pool. During the chase period, the simultaneous decrease of ^^N-incorporation in the soluble N fraction ,and increase in the insoluble N fraction in different pine parts, particularly in the needles, suggested that protein synthesis occurred mostly in young tissues of the shoot and was the major sink of the newly absorbed ^^N-NO3.

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Preparation of submicrogram nitrogen samples for isotope analysis by the GS1 emission spectrometer

Cited by 16 publications

References 9 publications

Effect of N Management on Root Yield and N Uptake of Radishes in Southern China

Effect of N Management on Root Yield and N Uptake of Radishes in Southern China

Mineralização do nitrogênio incorporado como material vegetal em três solos da Amazônia Central

Nitrate assimilation and nitrogen circulation in Austrian pine

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