Preparation of surfactant-stabilized gold nanoparticle–peptide nucleic acid conjugates

Duy, Janice; Connell, Laurie B.; Eck, Wolfgang; Collins, S.D.; Smith, Robert L.

doi:10.1007/s11051-010-9996-0

Cited by 39 publications

(34 citation statements)

References 23 publications

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“…Tween 20 (0.1%) was first added to AuNP suspension prior to addition of M2e peptides to provide a steric shield on gold surfaces, thus improving stability of the suspension and preventing large crosslinked aggregation of AuNPs from centrifugation at high speed [44]. To obtain full coverage of AuNP surfaces, M2e was added in a molar excess.…”

Section: Resultsmentioning

confidence: 99%

Gold Nanoparticle–M2E Conjugate Coformulated with Cpg Induces Protective Immunity Against Influenza A Virus

2014

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Aim: This study aimed to develop a novel influenza A vaccine by conjugating the highly conserved extracellular region of the matrix 2 protein (M2e) of influenza A virus to gold nanoparticles (AuNPs) and to test the vaccine in a mouse influenza challenge model. Materials & methods: Citrate-reduced AuNPs (diameter: 12 nm) were synthesized, and characterized by transmission electron microscopy and dynamic light scattering. M2e was conjugated to AuNPs through thiol–gold interactions to form M2e–AuNP conjugates. Particle stability was confirmed by UV–visible spectra, and M2e conjugation was further characterized by x-ray photoelectron spectroscopy. Mice were immunized with M2e–AuNPs with or without CpG (cytosine-guanine rich oligonucleotide) as an adjuvant with appropriate control groups. Sera was collected and M2e-specific immunoglobulin (IgG) was measured, and immunized mice were challenged with PR8-H1N1 influenza virus. Results: M2e-capped AuNPs could be lyophilized and stably resuspended in water. Intranasal vaccination of mice with M2e–AuNP conjugates induced M2e-specific IgG serum antibodies, which significantly increased upon addition of soluble CpG as adjuvant. Upon challenge with lethal PR8, mice vaccinated with M2e-AuNP conjugates were only partially protected, while mice that received soluble CpG as adjuvant in addition to M2e–AuNP were fully protected. Conclusion: Overall, this study demonstrates the potential of using the M2e–AuNP conjugates with CpG as an adjuvant as a platform for developing an influenza A vaccine.

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Section: Resultsmentioning

confidence: 99%

Gold Nanoparticle–M2E Conjugate Coformulated with Cpg Induces Protective Immunity Against Influenza A Virus

2014

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“…Synthetic polymers typically polyvinyl alcohol [1], polyvinyl pyrrolidone [2,3], polyethylene glycol [4], sodium polyacrylate [5,6], and surfactants [7,8] have been commonly used as stabilizers in the bottom-up approach of synthesis of gold nanoparticles (AuNPs). In addition, biopolymers such as chitosan [9][10][11], alginate [12,13], hyaluronan [14,15], gum arabic [16,17], protein [18,19], gelatin [20], dextran [21], and glucomannan [22] have been also used as stabilizers of colloidal AuNPs solution.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of Gold Nanoparticles Stabilized in Dextran Solution by Gamma Co-60 Ray Irradiation and Preparation of Gold Nanoparticles/Dextran Powder

Diem

Thao

Phú

et al. 2017

Journal of Chemistry

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Gold nanoparticles (AuNPs) in spherical shape with diameter of 6-35 nm stabilized by dextran were synthesized by -irradiation method. The AuNPs were characterized by UV-Vis spectroscopy and transmission electron microscopy. The influence of pH, Au 3+ concentration, and dextran concentration on the size of AuNPs was investigated. Results indicated that the smallest AuNPs size (6 nm) and the largest AuNPs size (35 nm) were obtained for pH of 1 mM Au 3+ /1% dextran solution of 5.5 and 7.5, respectively. The smaller Au 3+ concentration favored smaller size and conversely the smaller dextran concentration favored bigger size of AuNPs. AuNPs powders were prepared by spay drying, coagulation, and centrifugation and their sizes were also evaluated. The purity of prepared AuNPs powders was also examined by energy dispersive X-ray (EDX) analysis. Thus, the as-prepared AuNPs stabilized by biocompatible dextran in solution and/or in powder form can be potentially applied in biomedicine and pharmaceutics.

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“…The colloidal gold particles were observed to be spherical in shape, with an estimated diameter of 20 nm. The particles were well dispersed after conjugation with SPA, and no aggregates were observed in the solution, offering as an indicator for successful modification (Duy et al 2010).…”

Section: Resultsmentioning

confidence: 97%

A rapid one-step immunochromatographic test strip for rabies detection using canine serum samples

Tao

Li²

2014

Lett Appl Microbiol

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Significance and Impact of the Study: Simple and cheap techniques to detect rabies virus or monitor immunity against it are central in maintaining epidemiological control over the disease, particularly in endemic developing countries. While many techniques meet this requirement, they are confined to this usage as they are time-consuming and demand expensive instrumentation. Our immunochromatographic test strip can detect rabies antibody with high specificity and sensitivity; the output can be measured with naked eye. It allows safe and quick detection that will be of value in the surveillance of the immunization status of potential targets in rabies-endemic regions and will aid disease control. AbstractWe developed an immunochromatographic test strip using colloidal gold-coated staphylococcal protein A (SPA) for the detection of rabies antibody in canine serum samples. The recombinantly expressed rabies virus phosphoprotein (RV-P) and the anti-staphylococcal protein A (anti-SPA) polyclonal antibody were coated on the test (T) and control (C) lines on a nitrocellulose membrane, respectively. This layout is designed such that the polyclonal antibody in canine serum is captured by the colloidal gold-SPA conjugates, before the rabies antibody complex is specifically selected by the RV-P deposited on the T line, forming a 'sandwich' pattern. Unbound excess colloidal SPA then proceeds to the control line where SPA specifically interacts with the anti-SPA antibody, producing a red precipitation at the C line, indicating the validity of the strip. We tested 165 canine serum samples with the strips, and the results were compared with those obtained using ELISA. The specificity and sensitivity of ICTS were found to be 93Á1 and 92Á2%, respectively. As a rapid technique, not demanding expensive instrumentation, the strip offers potential in disease monitoring, especially in rabies-endemic developing countries.

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Preparation of surfactant-stabilized gold nanoparticle–peptide nucleic acid conjugates

Cited by 39 publications

References 23 publications

Gold Nanoparticle–M2E Conjugate Coformulated with Cpg Induces Protective Immunity Against Influenza A Virus

Gold Nanoparticle–M2E Conjugate Coformulated with Cpg Induces Protective Immunity Against Influenza A Virus

Synthesis of Gold Nanoparticles Stabilized in Dextran Solution by Gamma Co-60 Ray Irradiation and Preparation of Gold Nanoparticles/Dextran Powder

A rapid one-step immunochromatographic test strip for rabies detection using canine serum samples

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