Preparation of transforming deoxyribonucleic acid by phenol treatment

Saito, Hiuga; Miura, Kiyonori

doi:10.1016/0926-6550(63)90386-4

Cited by 1,709 publications

(228 citation statements)

References 21 publications

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“…Genomic DNA of G. mesophila KMM241 was prepared with the method described by Saito and Miura [18]. Blast analysis in NCBI showed that the 16S rRNA gene sequence of G. mesophila KMM241 had more than 99% identity to that of Pseudoalteromonas atlantica T6c.…”

Section: Methodsmentioning

confidence: 99%

Gene Cloning, Expression and Characterization of a Novel Xylanase from the Marine Bacterium, Glaciecola mesophila KMM241

Guo

Yong-sheng

et al. 2013

Marine Drugs

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Marine xylanases are rather less studied compared to terrestrial xylanases. In this study, a new xylanase gene, xynB, was cloned from the marine bacterium, Glaciecola mesophila KMM241, and expressed in Escherichia coli. xynB encodes a multi-domain xylanase XynB of glycoside hydrolase (GH) family 8. The recombinant XynB comprises an N-terminal domain (NTD) with unknown function and a catalytic domain, which is structurally novel among the characterized xylanases of GH family 8. XynB has the highest identity (38%) to rXyn8 among the characterized xylanases. The recombinant XynB showed maximal activity at pH 6–7 and 35 °C. It is thermolabile and salt-tolerant. XynB is an endo-xylanase that demands at least five sugar moieties for effective cleavage and to hydrolyze xylohexaose and xylopentaose into xylotetraose, xylotriose and xylobiose. NTD was expressed in Escherichia coli to analyze its function. The recombinant NTD exhibited a high binding ability to insoluble xylan and avicel and little binding ability to chitosan and chitin. Since the NTD shows no obvious homology to any known carbohydrate-binding module (CBM) sequence in public databases, XynB may contain a new type of CBM.

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Section: Methodsmentioning

confidence: 99%

Gene Cloning, Expression and Characterization of a Novel Xylanase from the Marine Bacterium, Glaciecola mesophila KMM241

Guo

Yong-sheng

et al. 2013

Marine Drugs

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“…For DNA base composition and DNA-DNA hybridization test, the DNA was extracted from cells harvested from MRS broth culture which had been incubated for 8 h at 30°C. It was purified by the procedure of Saitou and Miura (1963). DNA base composition was determined by the method of Tamaoka and Komagata (1984) by using high-performance liquid chromatography (HPLC) following enzymic digestion of DNA to deoxyribonucleosides.…”

Section: Dna-dna Relatedness Analysis Of Selected Strainsmentioning

confidence: 99%

Characterization and application of lactic acid bacteria for tropical silage preparation

Pholsen

Khota

Pang

et al. 2016

Animal Science Journal

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Strains TH 14, TH 21 and TH 64 were isolated from tropical silages, namely corn stover, sugar cane top and rice straw, respectively, prepared in Thailand. These strains were selected by low pH growth range and high lactic acid-producing ability, similar to some commercial inoculants. Based on the analysis of 16S ribosomal RNA gene sequence and DNA-DNA relatedness, strain TH 14 was identified as Lactobacillus casei, and strains TH 21 and TH 64 were identified as L. plantarum. Strains TH 14, TH 21, TH 64 and two commercial inoculants, CH (L. plantarum) and SN (L. rhamnosus), were used as additives to fresh and wilted purple Guinea and sorghum silages prepared using a small-scale fermentation method. The number of epiphytic lactic acid bacteria (LAB) in the forages before ensilage was relatively low but the numbers of coliform and aerobic bacteria were higher. Sorghum silages at 30 days of fermentation were all well preserved with low pH (3.56) and high lactic acid production (72.86 g/kg dry matter). Purple Guinea silage inoculated with LAB exhibited reduced count levels of aerobic and coliform bacteria, lower pH, butyric acid and ammonia nitrogen and increased lactic acid concentration, compared with the control. Strain TH 14 more effectively improved lactic acid production compared with inoculants and other strains. © 2016 Japanese Society of Animal Science.

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“…Cells grown for 8 h in MRS broth at 30°C were used for DNA extraction and purification as described by (Saitou and Miura (1963). The 16S ribosomal RNA (rRNA) gene sequence coding region was amplified by PCR performed in a PCR ThermalCycler (Takara Shuzo Co., Ltd, Ohtsu, Japan).…”

Section: S Rrna Gene Sequencingmentioning

confidence: 99%

Identification of lactic acid bacteria isolated from corn stovers

Pang

Zhang

Guangyong

et al. 2011

Animal Science Journal

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One hundred and twenty-six strains were isolated from corn stover in Henan Province, China, of which 105 isolates were considered to be lactic acid bacteria (LAB) according to Gram-positive, catalase-negative and mainly metabolic lactic acid product. Analysis of the 16S ribosomal DNA sequence of 21 representative strains was used to confirm the presence of the predominant groups and to determine the phylogenetic affiliation of isolates. The sequences from the various LAB isolates showed high degrees of similarity to those of the GenBank type strains between 99.4% and 100%. The prevalent LAB, predominantly Lactobacillus (85.6%), consisted of L. plantarum (33.3%), L. pentosus (28.6%) and L. brevis (23.7%). Other LAB species as Leuconostoc lactis (4.8%), Weissella cibaria (4.8%) and Enterococcus mundtii (4.8%) also presented in corn stover. The present study is the first to fully document corn stover-associated LAB involved in the silage fermentation. The identification results revealed LAB composition inhabiting corn stover and enabling the future design of appropriate inoculants aimed at improving the fermentation quality of silage.

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Preparation of transforming deoxyribonucleic acid by phenol treatment

Cited by 1,709 publications

References 21 publications

Gene Cloning, Expression and Characterization of a Novel Xylanase from the Marine Bacterium, Glaciecola mesophila KMM241

Gene Cloning, Expression and Characterization of a Novel Xylanase from the Marine Bacterium, Glaciecola mesophila KMM241

Characterization and application of lactic acid bacteria for tropical silage preparation

Identification of lactic acid bacteria isolated from corn stovers

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