Preparation of Zr<sup>4+</sup> affinity column by atom transfer radical polymerization for phosphoprotein isolation

Park, Jung Hyun; Bae, In-Ae; Choi, Seong‐Ho

doi:10.1002/app.30473

Cited by 3 publications

(1 citation statement)

References 45 publications

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“…Glycidyl methacrylate (GMA) is one of the monomers which are easily modified into various functional groups. As GMA is polymerized, the epoxy group assists GMA becoming useful for the introduction of various functional groups, such as amines, 11,12 amino acid, 13 phosphoric acid, 14 and so on. 15 However, the amine with ion-liquid properties has not been reported until now.…”

Section: Resultsmentioning

confidence: 99%

Convenient Preparation of Lon-Exchange PVdF Membranes by a Radiation-Induced Graft Polymerization for a Battery Separator

Kim¹,

Ryu²,

Kwen³

et al. 2010

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초록: 메탄올 용매에서 고분자 촉진 단량체와 소디윰 스티렌 슐포네이트를 방사선 그래프트 방법으로 양이온 교 환 PVdF 맴브레인을 제조하였다. 고분자 촉진 단량체로서 스티렌, 아크릴산, 비닐 피롤리돈을 사용하였다. 또한, 음이온 교환 PVdF 맴브레인도 방사선 그래프트 중합법에 의해 제조하였다. 양이온 및 음이온 교환 PVdF 맴브레인은 SEM, XPS 그리고 열분석기기를 통해 특성평가를 하였고 성공적으로 합성됨을 확인할 수 있었다. 그래프트 수율, 이온교환기 의 양 및 침투율은 각각 30.0∼32.3%, 2.81∼3.01 mmol/g 그리고 66.6∼147%로 평가되었으며, 20 ℃에서 이온 전 도도를 측정한 결과 0.020∼0.053 S/cm 이었다. 최종적으로, 제조된 양이온 및 음이온교환 PVdF 맴브레인은 전지 격막으로서 충분히 사용될 수 있음을 확인할 수 있었다.Abstract: A cation-exchange nanofiber poly(vinylidene fluoride) (PVdF) membrane was prepared by a radiation-induced graft polymerization (RIGP) of sodium styrene sulfonate (NaSS) in the presence of the polymerizable access agents in methanol solution. The used polymerizable access agents include styrene, acrylic acid, and vinyl pyrrolidone. The anion-exchange nanofiber PVdF membrane was also prepared by RIGP of glycidyl methacrylate (GMA) and its subsequent chemical modification. The successful preparations of cation-and anion-exchange PVdF membranes were confirmed via SEM,XPS and thermal analysis. The content of the grafting yield, ion-exchange group, and water uptake was in the range of 30.0∼32.3%, 2.81∼3.01 mmol/g and 66.6∼147%, respectively. The proton conductivity at 20 ℃ was in the range of 0.020∼0.053 S/cm. From the result, the prepared ionexchange PVdF membrane can be used as a separator in battery cells.

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Section: Resultsmentioning

confidence: 99%

Convenient Preparation of Lon-Exchange PVdF Membranes by a Radiation-Induced Graft Polymerization for a Battery Separator

Kim¹,

Ryu²,

Kwen³

et al. 2010

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Synthesis of a chiral stationary phase with poly[styrene‐b‐cellulose 2,3‐bis(3,5‐dimethylphenylcarbamate)] by surface‐initiated atom transfer radical polymerization and its chiral resolution efficiency

Yang

Choi

2011

J of Applied Polymer Sci

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A chiral stationary phase (CSP) with poly[-styrene-b-cellulose 2,3-bis(3,5-dimethylphenylcarbamate)] was synthesized by the surface-initiated atom transfer radical polymerization (SI-ATRP) of cellulose 2,3-bis(3,5-dimethylphenylcarbamate)-6-acrylate after the SI-ATRP of styrene on the surface of silicon dioxide supports in pyridine. The successful preparation of the CSP with poly[styrene-b-cellulose 2,3-bis(3,5-dimethylphenylcarbamate)] was confirmed via Fourier transform infrared spectroscopy, field emission scanning electron microscopy, X-ray photoelectron spectroscopy, elemental analysis, and thermal analysis. The applicability for the chiral resolution of the CSP with poly[styrene-b-cellulose 2,3-bis(3,5-diphenylcarbamate)] was evaluated with high-performance liquid chromatography with 10 racemates under various mobile phases of hexane/alcohol, hexane/tetrahydrofuran (THF), and hexane/chloroform. The results show that the CSP with poly[styrene-b-cellulose 2,3-bis(3,5-diphenylcarbamate)] could be used in THF and chloroform as eluents. The chiral resolutions of the commercial Chiracel OD, the CSP with cellulose 2,3-bis(3,5-dimethylphenylcarabmate), and the CSP with poly[styrene-b-cellulose 2,3-bis(3,5-dimethylphenylcarbamate)] prepared by SI-ATRP were examined.

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Metal-Enzyme Frameworks: Role of Metal Ions in Promoting Enzyme Self-Assembly on α-Zirconium(IV) Phosphate Nanoplates

et al. 2013

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Previously, an ion-coupled protein binding (ICPB) model was proposed to explain the thermodynamics of protein binding to negatively charged α-Zr(IV) phosphate (α-ZrP). This model is tested here using glucose oxidase (GO) and met-hemoglobin (Hb) and several cations (Zr(IV), Cr(III), Au(III), Al(III), Ca(II), Mg(II), Zn(II), Ni(II), Na(I), and H(I)). The binding constant of GO with α-ZrP was increased ∼380-fold by the addition of either 1 mM Zr(IV) or 1 mM Ca(II), and affinities followed the trend Zr(IV) ≃ Ca(II) > Cr(III) > Mg(II) ≫ H(I) > Na(I). Binding studies could not be conducted with Au(III), Al(III), Zn(II), Cu(II), and Ni(II), as these precipitated both proteins. Zr(IV) increased Hb binding constant to α-ZrP by 43-fold, and affinity enhancements followed the trend Zr(IV) > H(I) > Mg(II) > Na(I) > Ca(II) > Cr(III). Zeta potential studies clearly showed metal ion binding to α-ZrP and affinities followed the trend, Zr(IV) ≫ Cr(III) > Zn(II) > Ni(II) > Mg(II) > Ca(II) > Au(III) > Na(I) > H(I). Electron microscopy showed highly ordered structures of protein/metal/α-ZrP intercalates on micrometer length scales, and protein intercalation was also confirmed by powder X-ray diffraction. Specific activities of GO/Zr(IV)/α-ZrP and Hb/Zr(IV)/α-ZrP ternary complexes were 2.0 × 10(-3) and 6.5 × 10(-4) M(-1) s(-1), respectively. While activities of all GO/cation/α-ZrP samples were comparable, those of Hb/cation/α-ZrP followed the trend Mg(II) > Na(I) > H(I) > Cr(III) > Ca(II) ≃ Zr(IV). Metal ions enhanced protein binding by orders of magnitude, as predicted by the ICPB model, and binding enhancements depended on charge as well as the phosphophilicity/oxophilicity of the cation.

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Preparation of Zr⁴⁺ affinity column by atom transfer radical polymerization for phosphoprotein isolation

Cited by 3 publications

References 45 publications

Convenient Preparation of Lon-Exchange PVdF Membranes by a Radiation-Induced Graft Polymerization for a Battery Separator

Convenient Preparation of Lon-Exchange PVdF Membranes by a Radiation-Induced Graft Polymerization for a Battery Separator

Synthesis of a chiral stationary phase with poly[styrene‐b‐cellulose 2,3‐bis(3,5‐dimethylphenylcarbamate)] by surface‐initiated atom transfer radical polymerization and its chiral resolution efficiency

Metal-Enzyme Frameworks: Role of Metal Ions in Promoting Enzyme Self-Assembly on α-Zirconium(IV) Phosphate Nanoplates

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Preparation of Zr4+ affinity column by atom transfer radical polymerization for phosphoprotein isolation

Cited by 3 publications

References 45 publications

Convenient Preparation of Lon-Exchange PVdF Membranes by a Radiation-Induced Graft Polymerization for a Battery Separator

Convenient Preparation of Lon-Exchange PVdF Membranes by a Radiation-Induced Graft Polymerization for a Battery Separator

Synthesis of a chiral stationary phase with poly[styrene‐b‐cellulose 2,3‐bis(3,5‐dimethylphenylcarbamate)] by surface‐initiated atom transfer radical polymerization and its chiral resolution efficiency

Metal-Enzyme Frameworks: Role of Metal Ions in Promoting Enzyme Self-Assembly on α-Zirconium(IV) Phosphate Nanoplates

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Preparation of Zr⁴⁺ affinity column by atom transfer radical polymerization for phosphoprotein isolation