Preparation, physicochemical characterization and biological evaluation of cefodizime metal ion complexes

Abstract

Objectives Cefodizime is a broad spectrum cephalosporin belonging to the third generation agents. In this study, attention has been paid to the preparation, physicochemical characterization and biological evaluation of new Cu 2+ , Zn 2+ , Fe 3+ , Co 2+ and Al 3+ complexes of cefodizime. Methods The stoichiometrics and the mode of bonding of the complexes were deduced from their elemental and metal analysis, electrical conductivity measurements, UV-vis, infrared and Raman spectroscopic investigations. Study of the stoichiometry of these complexes referred to the formation of 1 : 1 ratios of metal to ligand. Antimicrobial activity of the complexes was determined using two strains of Gram-positive (Bacillus subtilis and Proteus vulgaris) and two strains of Gram-negative (Escherichia coli W3110 and Pseudomonas putida) bacteria. The minimal inhibitory concentration was determined as the lowest concentration inhibiting bacterial growth on solid Luria Bertani medium. Key findings The spectra gave evidence as to the position of binding. In addition, the aqueous solubility of cefodizime was strongly reduced by complexation. Conclusions The antibacterial activity of cefodizime was not affected by complexation with Al 3+ but it was reduced by complexation with the other tested metal ions against the bacteria under study.

A High-Throughput Enzyme-Coupled Assay for SAMHD1 dNTPase

“…Some cephalosporin antibiotics are known to form tight complexes with divalent metal ions, 27 including Zn 2+ , which suggested that the inhibition might result from an E-Zn 2+ –Ceph C enzyme-metal-bridged complex. However, further investigation showed that Zn 2+ alone (as the chloride salt) was also inhibitory (IC 50 Zn = 2.1 ± 0.2 µM, h = 1.2 ± 0.1; Fig.…”