Preparation, radiolabeling with 99mTc and 67Ga and biodistribution studies of albumin nanoparticles coated with polymers

Arcocha-Torres, M. de; Quincoces, Gemma; Martínez‐López, Ana L.; Erhard, A.; Collantes, María; Martínez-Rodríguez, I.; Ecay, Margarita; Banzo, Ignacio; Irache, J.M.; Peñuelas, Iván

doi:10.1016/j.remnie.2020.04.002

Cited by 3 publications

(5 citation statements)

References 33 publications

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“…Animals injected with free 67 Ga (control) showed uptake in the bloodstream, liver, lacrimal and salivary glands ( Figure 4B ), according previous reports. 34 , 47 We can also observe free 67 Ga in the bladder and kidneys, a consequence of renal clearance. In comparison, NOTA-SNs showed similar biodistribution than observed in PET/CT images, with main accumulation in liver and RES organs, and its intensity decreases over time.…”

Section: Resultsmentioning

confidence: 83%

“…Then, the free radionuclide was removed by filtration in PD-10 columns, obtaining a 80 ± 2% of RCY ( Figure 4A , before incubation with serum), between 10% and 20% higher than polymer and protein-based nanoparticles previously reported. 34 , 46 The measured RCP was 98.9%, conducted by ITLC ( Table S2, Supporting Information ). In addition, RCS studies proved that the labeling was highly stable in serum over 72 h ( Figure 4A ).…”

Section: Resultsmentioning

confidence: 99%

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Biodistribution of 68/67Ga-Radiolabeled Sphingolipid Nanoemulsions by PET and SPECT Imaging

Díez‐Villares¹,

Pellico²,

Gómez-Lado³

et al. 2021

IJN

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Background and Purpose Non-invasive imaging methodologies, especially nuclear imaging techniques, have undergone an extraordinary development over the last years. Interest in the development of innovative tracers has prompted the emergence of new nanomaterials with a focus on nuclear imaging and therapeutical applications. Among others, organic nanoparticles are of the highest interest due to their translational potential related to their biocompatibility and biodegradability. Our group has developed a promising new type of biocompatible nanomaterials, sphingomyelin nanoemulsions (SNs). The aim of this study is to explore the potential of SNs for nuclear imaging applications. Methods Ready-to-label SNs were prepared by a one-step method using lipid derivative chelators and characterized in terms of their physicochemical properties. Stability was assessed under storage and after incubation with human serum. Chelator-functionalized SNs were radiolabeled with 67 Ga and 68 Ga, and the radiochemical yield (RCY), radiochemical purity (RCP) and radiochemical stability (RCS) were determined. Finally, the biodistribution of 67/68 Ga-SNs was evaluated in vivo and ex vivo. Results Here, we describe a simple and mild one-step method for fast and efficient radiolabeling of SNs with 68 Ga and 67 Ga radioisotopes. In vivo experiments showed that 67/68 Ga-SNs can efficiently and indistinctly be followed up by PET and SPECT. Additionally, we proved that the biodistribution of the 67/68 Ga-SNs can be conveniently modulated by modifying the surface properties of different hydrophilic polymers, and therefore the formulation can be further adapted to the specific requirements of different biomedical applications. Conclusion This work supports 67/68 Ga-SNs as a novel probe for nuclear imaging with tunable biodistribution and with great potential for the future development of nanotheranostics.

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Section: Resultsmentioning

confidence: 83%

Section: Resultsmentioning

confidence: 99%

Biodistribution of 68/67Ga-Radiolabeled Sphingolipid Nanoemulsions by PET and SPECT Imaging

Díez‐Villares¹,

Pellico²,

Gómez-Lado³

et al. 2021

IJN

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“…The proportion of the administered dose per gram of organ was used to indicate the amount of the radiolabeled compound that was administered into the various organs. 67–70…”

Section: Methodsmentioning

confidence: 99%

An 18 kDa TSPO specific ligand-based polymeric nanoformulation: synthesis, computational and biological studies

Singh,

Kakkar

et al. 2024

New J. Chem.

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“…Thus, the biodistribution profile of the 99m Tc -GPM2 and 67 Ga -NOTA-GPM2-based NPs systems showed major accumulation in the liver 2 and 24 h after i.v. administration [78].…”

Section: Oligomersmentioning

confidence: 99%

“…Thus, the biodistribution profile of the 99m Tc -GPM2 and 67 Ga -NOTA-GPM2-based NPs systems showed major accumulation in the liver 2 and 24 h after i.v. administration [78]. Chelator-based radiolabeling using HYNIC and stannous chloride (reducing agent) [58] A549 tumor imaging Without drug Direct radiolabeling using stannous chloride (reducing agent) [59] Polymeric Nanoparticles General biodistribution study Etoposide Direct radiolabeling using stannous chloride (reducing agent) [60] Sentinel lymph node Without drug Direct radiolabeling using stannous chloride (reducing agent),chelator-based radiolabeling using DTPA [61] Comparative study using various reducing agents for 99m Tc radiolabeling…”

Section: Protein Nanoparticlesmentioning

confidence: 99%

Recent Progress in Technetium-99m-Labeled Nanoparticles for Molecular Imaging and Cancer Therapy

et al. 2021

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Nanotechnology has played a tremendous role in molecular imaging and cancer therapy. Over the last decade, scientists have worked exceptionally to translate nanomedicine into clinical practice. However, although several nanoparticle-based drugs are now clinically available, there is still a vast difference between preclinical products and clinically approved drugs. An efficient translation of preclinical results to clinical settings requires several critical studies, including a detailed, highly sensitive, pharmacokinetics and biodistribution study, and selective and efficient drug delivery to the target organ or tissue. In this context, technetium-99m (99mTc)-based radiolabeling of nanoparticles allows easy, economical, non-invasive, and whole-body in vivo tracking by the sensitive clinical imaging technique single-photon emission computed tomography (SPECT). Hence, a critical analysis of the radiolabeling strategies of potential drug delivery and therapeutic systems used to monitor results and therapeutic outcomes at the preclinical and clinical levels remains indispensable to provide maximum benefit to the patient. This review discusses up-to-date 99mTc radiolabeling strategies of a variety of important inorganic and organic nanoparticles and their application to preclinical imaging studies.

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Preparation, radiolabeling with 99mTc and 67Ga and biodistribution studies of albumin nanoparticles coated with polymers

Cited by 3 publications

References 33 publications

Biodistribution of 68/67Ga-Radiolabeled Sphingolipid Nanoemulsions by PET and SPECT Imaging

Biodistribution of 68/67Ga-Radiolabeled Sphingolipid Nanoemulsions by PET and SPECT Imaging

An 18 kDa TSPO specific ligand-based polymeric nanoformulation: synthesis, computational and biological studies

Recent Progress in Technetium-99m-Labeled Nanoparticles for Molecular Imaging and Cancer Therapy

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