Preparation, statistical optimisation andin vitrocharacterisation of poly (3-hydroxybutyrate-co-3-hydroxyvalerate)/poly (lactic-co-glycolic acid) blend nanoparticles for prolonged delivery of teriparatide

Abstract

The purpose of this study was the preparation, optimisation and in vitro characterisation of PHBV and PLGA blend nanoparticles (NPs) for prolonged delivery of Teriparatide. Double emulsion solvent evaporation technique was employed for the fabrication of NPs. The nanoformulation was optimised using the Box-Behnken methodology. The morphological properties of NPs were assessed by both SEM and transmission electron microscopy (TEM). Encapsulation of Teriparatide within the NPs and lacking of chemical bonds between drug and copolymers were proved by XRPD, FTIR and DSC. The structural stability of Teriparatide after processing was confirmed by fluorescence spectrometry. The average size of optimised NPs was 250.0 nm with entrapment efficiency (EE) of 89.5% and drug loading (DL) of 5.0%. Teriparatide release from optimised NPs led to 64.4% release over 30 days and it showed a diffusion-based mechanism. Based on the favourable results, PHBV/PLGA blend NPs could be a promising candidate for designing a controlled release formulation of Teriparatide.

The Local Release of Teriparatide Incorporated in 45S5 Bioglass Promotes a Beneficial Effect on Osteogenic Cells and Bone Repair in Calvarial Defects in Ovariectomized Rats

“…While the FT-IR analyses indicated great structural similarity between the 45S5 bioglass and BG groups, similar to the deconvolutions of the FTIR spectra in the study by Lee et al [ 77 ], who showed peaks associated with the structure of the bioglass, the BGT group was different, showing bands from the standard amide group (1660 cm −1 ) of the teriparatide drug molecule. This same band was observed in the study by Bahari Javan et al [ 79 ]. A decrease in the band of the group not bound to oxygen (NBO) can be observed; these bands are necessary for the dissolution of the bioglass, which will result in the formation of a layer of apatite hydroxycarbonate (HCA) on the surface of the material when in contact with fluids body or aqueous media.…”

Fabrication, Optimization, and Evaluation of Paclitaxel and Curcumin Coloaded PLGA Nanoparticles for Improved Antitumor Activity

“…Drug release data were fitted into mathematical models of zero-order, first-order, Higuchi, and Korsmeyer–Peppas using the DD solver, an add-in of MS Excel, to build regression analysis of the mathematical expressions to explain the mechanism of drug release. − …”

Fe 3 O 4 @MCM-41-Hydroxyapatite-Teriparatide nanocomposite: as a highly efficient drug delivery system for bone tissue

“…In g.1 (b), because of the Teriparatide present, a strong amide connection with the C = O group is located at 1492 cm -1 . The interaction between the N-H bending and the C-N stretching of the C-N-H group results in the 1435 cm -1 stretching bond [21].…”

“…However, other drugs have antiresorptive effects and prevent bone destruction. While the only FDA-approved anabolic drugs for the treatment of postmenopausal osteoporosis are Teriparatide (PTH ) and Abaloparatide, a PTHrP (parathyroid hormone-related protein), [14,15], Teriparatide is one of the systemic drugs whose disadvantages in using high doses are aggravated drug-induced side effects and the risk of osteosarcoma [16][17][18][19] and includes precautions for use in people with a history of kidney disease, hypercalcemia, and cardiovascular disease [20], and also the problems of intermittent use during the 2-year treatment period [21].…”