Preparative Purification of Bioactive Compounds from Flos Chrysanthemi Indici and Evaluation of Its Antiosteoporosis Effect

Abstract

To understand the material basis and underlying molecular machinery of antiosteoporosis activity of the Flos Chrysanthemi Indici (FCI), the consequences of ethanol extract on the bone loss in mice induced due to ovariectomy (OVX) was evaluated. Also, the antiosteoporosis fraction obtained from the FCI ethanol extract was isolated and purified using a preparative high-speed countercurrent chromatography (HSCCC). The in vitro impact of the compounds was investigated on osteoblast proliferation and differentiation. The results revealed that ethyl acetate fraction with robust in vivo antiosteoporosis activity was obtained. The important compounds purified by HSCCC using gradient elution system included acacetin, apigenin, luteolin, and linarin. The four compounds enhanced the differentiation and proliferation of osteoblasts in MC3T3-E1 cells. They also augmented the mRNA levels of runt-related transcription factor 2 (Runx2), osteocalcin (OCN), osteopontin (OPN), and type I collagen (COL I). The AKT signaling pathway was also activated in MC3T3-E1 cells by the four compounds. The present study demonstrated that the antiosteoporosis effects of FCI did not depend on a single component, and HSCCC efficiently isolated and purified the antiosteoporosis bioactive compounds from FCI.

Luteolin supports osteogenic differentiation of human periodontal ligament cells

“…RUNX2 plays a significant role in the early proliferation of osteoblasts [37], and OSX is seated downstream of RUNX2 and also plays a key role in late osteoblastic differentiation and maturation [38]. Jia LI et al [39] showed that luteolin could not only promote the osteogenic differentiation and proliferation of MC3T3-E1 cells, but also up-regulate the mRNA expression levels of RUNX2 and OCN. In this study, we demonstrated that luteolin at 0.01, 0.1, 1 μmol/L increased the correlative mRNA and protein expressions of BMP2, OCN, RUNX2 and OSX, and 1 μmol/L luteolin showed a relatively high performnace.…”

Optimization of extraction of linarin from Flos chrysanthemi indici by response surface methodology and artificial neural network

“…Flos chrysanthemi indici , anthotaxy of Chrysanthemum indicum L . is a traditional Chinese herb, which has many beneficial effects such as heat‐clearing and detoxication, analgesic, and anti‐inflammation . Flavonoids are main active substances in Flos chrysanthemi indici .…”

Linarin, a Glycosylated Flavonoid, with Potential Therapeutic Attributes: A Comprehensive Review

“…They also improved the mRNA levels of runt-related transcription factor 2 (RUNX2), osteocalcin (OCN), osteopontin (OPN), and type I collagen. The AKT signaling pathway was also activated in MC3T3-E1 cells by the four compounds [39].…”

“…In the study of , high-speed counter-current chromatography (HSCCC) was applied in order to isolate this flavonoid from the hydro-ethanolic extract (80%) of Flos Chrysanthemi indici [39], however, it has also been identified from this species as reported by three other groups [40][41][42].…”

“…The flower hydro-ethanolic extract (80%) was analyzed via HPLC-UV and LN was accordingly quantified (32.8 mg/g) [39]. The impacts of several extraction conditions on LN contents of the C. indicum flower ethanolic extract [40] have been explored; the highest LN yield (88.11%) was measured in the plant samples extracted with 80% ethanol, 2 h of extraction, extraction frequency of three, and solvent to material ratio of 12 mL/g [40].…”