Preparative separation and purification of liensinine, isoliensinine and neferine from seed embryo of <i>Nelumbo nucifera</i> GAERTN using high‐speed counter‐current chromatography

Shao, Lei; Wang, Bin; Li, Xinzhong; Qi, Long-feng; Liang, Yi‐Zeng

doi:10.1002/jssc.200800766

Cited by 59 publications

(35 citation statements)

References 21 publications

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“…There have several analytical methods been published for simultaneous determination of liensinine, isoliensinine and neferine in the plants and biological samples, including HPLC (Du et al, 2011;Huang et al, 2011), HSCCC (Liu et al, 2009), LC-MS/ MS (Chen et al, 2007;Zhou et al, 2007;Yu et al, 2013) and LC-TOF/ MS (Lin et al, 2013). However, to our best knowledge, there are only two HPLC bioanalytical methods for simultaneous determination of the three alkaloids in biological fluids, but the methods required elaborate sample preparation, long analytical time, and time-consuming (Du et al, 2011;Huang et al, 2011).…”

Section: Introductionmentioning

confidence: 95%

Simultaneous determination of liensinine, isoliensinine and neferine in rat plasma by UPLC–MS/MS and application of the technique to pharmacokinetic studies

Dong

et al. 2015

Journal of Ethnopharmacology

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Section: Introductionmentioning

confidence: 95%

Simultaneous determination of liensinine, isoliensinine and neferine in rat plasma by UPLC–MS/MS and application of the technique to pharmacokinetic studies

Dong

et al. 2015

Journal of Ethnopharmacology

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“…(Liu et al 2009). Accumulating studies have demonstrated that N. nucifera has multiple protective effects on cardiovascular diseases such as hypertension, arrhythmia, and atherosclerosis as well as on obesity (Chen et al 1962;Qian 2002;Ono et al 2006;Ho et al 2009;Pan et al 2009).…”

Section: Introductionmentioning

confidence: 93%

“…A preparative high-speed counter-current chromatography method for separation and purification of neferine from seed embryo of N. nucifera was successfully established by using n-hexane -ethyl acetate -methanol -water (5:8:4:5, by volume, containing 0.5% NH 4 OH) as the two-phase solvent system (Liu et al 2009). From 200 mg of crude extract, 18.4 mg of 58.4 mg neferine was obtained with a purity of 98.6%.…”

Section: Preparation Of Neferinementioning

confidence: 99%

Protective effect of neferine on endothelial cell nitric oxide production induced by lysophosphatidylcholine: the role of the DDAH–ADMA pathway

Peng

Zhang

Shao

et al. 2011

Can. J. Physiol. Pharmacol.

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Neferine, extracted from the seed embryo of Nelumbo nucifera Gaertn., has multiple cardiovascular pharmacological effects. The dimethylarginine dimethylaminohydrolase (DDAH) - asymmetric dimethylarginine (ADMA) system is a novel pathway for modulating nitric oxide (NO) production. The aim of this study was to investigate whether the protective effect of neferine on endothelial NO production was related to the DDAH-ADMA pathway. Human umbilical vein endothelial cells (HUVECs) were first exposed to neferine (0.1, 1.0, or 10.0 μmol/L) for 1 h, and then incubated with lysophosphatidylcholine (LPC; 10 μg/mL) in the presence of neferine for 24 h. The medium was collected for measuring the levels of NO, maleic dialdehyde (MDA), as well as ADMA. The endothelial cells were collected for measuring DDAH activity and the level of reactive oxygen species (ROS). LPC significantly decreased NO concentration and DDAH activity and increased the levels of ADMA, ROS, and MDA. Neferine could partially counteract the changes induced by LPC. These findings suggested that neferine could modulate the DDAH-ADMA pathway via its antioxidant properties, which was involved in its beneficial effect on endothelial NO production.

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“…But recent years that quality judgment by determining selecting one or two markers ignoring the complexity of the herbal medications is no longer the keynote of people's thinking pattern nowadays, and have acquired consensus that the comprehensive strategy for assessing the intact quality of herbal medicine is necessary. Consequently, Chromatographic fingerprinting and simultaneous determination of multiple components are the current trends [10][11][12]. Moreover, in order to reveal the working mechanisms of TCMs and to further explore the features in the chromatographic profile responsible for the bioactivity the techniques developed recently in systems biology, such as metabolomics and proteomics, seem to be necessary to be introduced into the research field in modernization of TCMs.…”

Section: Introductionmentioning

confidence: 99%

Chromatographic Fingerprinting and Metabolomics for Quality Control of TCM

Liang¹,

Xie²,

Chan³

2010

CCHTS

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Chromatographic fingerprinting technique of traditional Chinese medicine (TCM) has proved to be a comprehensive strategy for assessing the intact quality of herbal medicine. In general, one could use the chromatographic techniques to obtain a relatively complete picture of herbal medicines, which are in common called chromatographic fingerprints of herbal medicines to represent the so-called phytoequivalence. Based on this, the features of chromatographic fingerprints of herbal medicines have been discussed in some detail. The technique based on chromatographic fingerprinting is essentially a kind of high-throughput and integral tools to explore the complexity of herbal medicines. In order to further control the comprehensive quality of TCMs, some new strategies are proposed to trace the chemical changes of chromatographic fingerprints both in product processing and/or after their administration by modern chromatographic techniques and chemometrics. Combined with metabolomics, it seems possible for one to reveal the working mechanism of TCMs and to further control their intrinsic quality. Finally, the intensive study of chromatographic fingerprinting coupled with multivariate analysis tools developed in bioinformatics and chemometrics are emphasized in order to achieve the aim to reveal the working mechanisms of TCMs and to further control and strengthen TCMs' intrinsic quality in a comprehensive manner.

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Preparative separation and purification of liensinine, isoliensinine and neferine from seed embryo of Nelumbo nucifera GAERTN using high‐speed counter‐current chromatography

Cited by 59 publications

References 21 publications

Simultaneous determination of liensinine, isoliensinine and neferine in rat plasma by UPLC–MS/MS and application of the technique to pharmacokinetic studies

Simultaneous determination of liensinine, isoliensinine and neferine in rat plasma by UPLC–MS/MS and application of the technique to pharmacokinetic studies

Protective effect of neferine on endothelial cell nitric oxide production induced by lysophosphatidylcholine: the role of the DDAH–ADMA pathway

Chromatographic Fingerprinting and Metabolomics for Quality Control of TCM

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