PREPARATIVE SEPARATION OF HYDROXYL SAFFLOWER YELLOW A AND ANHYDROSAFFLOR YELLOW B IN PLANT EXTRACT OF <i>CARTHAMUS TINCTORIUS L.</i> BY REVERSE-PHASE MEDIUM-PRESSURE LIQUID CHROMATOGRAPHY

Zong, Xiaofei; Li, Li; Zhang, Huirong; Li, Bo; Liu, Chunming

doi:10.1080/10826076.2012.704614

Cited by 9 publications

(2 citation statements)

References 6 publications

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“…The results of the antioxidant assay were consistent with the experimental results reported by Yao et al [6]. The antioxidant activity increased with the increase of the concentration of safflower extract; meanwhile, the contents of HSYA and AHSYB in high concentration safflower extracts were also relatively high [16,44]. This indicates that HSYA and AHSYB played an important role in the antioxidant activity of the safflower extract.…”

Section: Discussionsupporting

confidence: 89%

Simultaneous Optimization of the Ultrasonic Extraction Method and Determination of the Antioxidant Activities of Hydroxysafflor Yellow A and Anhydrosafflor Yellow B from Safflower Using a Response Surface Methodology

Zhang

Jin

et al. 2020

Molecules

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An evaluation of the ultrasonic extraction process and the antioxidant activities of hydroxysafflor yellow A (HSYA) and anhydrosafflor yellow B (AHSYB) from safflower are presented herein. Using response surface methodology (RSM), based on a four-factor-three-level Box–Behnken design (BBD), the extraction parameters, namely, temperature, extraction time, solvent-to-material ratio, and extraction power, were optimized for maximizing the yields of HSYA and AHSYB. The maximum yield was obtained at a temperature of 66 °C with an extraction time of 36 min, solvent-to-material ratio of 16 mL/g, and the extraction power of 150 W, which was adjusted according to the actual conditions. The HSYA and AHSYB contents were determined using high performance liquid chromatography (HPLC). The yield and the comprehensive evaluation value of HSYA and AHSYB were calculated. The antioxidant activities of the extracts were determined using a ferric reducing antioxidant power (FRAP) kit and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity. The results suggested that the safflower extracts possessed obvious ferric reducing and DPPH radical scavenging activities. The antioxidant activity increased with increasing concentration. The results suggested that optimizing the conditions of ultrasonic extraction using RSM can significantly increase the yields of HSYA and AHSYB from safflower. The safflower extracts showed better antioxidant activity. This study can encourage future research on cardiovascular and cerebrovascular diseases.

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Section: Discussionsupporting

confidence: 89%

Simultaneous Optimization of the Ultrasonic Extraction Method and Determination of the Antioxidant Activities of Hydroxysafflor Yellow A and Anhydrosafflor Yellow B from Safflower Using a Response Surface Methodology

Zhang

Jin

et al. 2020

Molecules

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“…Compound ( 3 ) was tentatively identified as methyl succinic acid with a predicted mass of 132.04 mDa (C 5 H 8 O 4 ). In the mass spectrum of compound ( 3 ), the major fragment ions at 101, 85 and 59 appeared, which were characteristic fragmentation peaks for this substance [ 40 ]. Compound ( 4 ) was detected with an observed mass of 313.11 mDa (C 14 H 19 NO 7 ).…”

Section: Resultsmentioning

confidence: 99%

Cassia fistula Leaves; UHPLC-QTOF-MS/MS Based Metabolite Profiling and Molecular Docking Insights to Explore Bioactives Role towards Inhibition of Pancreatic Lipase

Aabideen

Mumtaz

Akhtar

et al. 2021

Plants

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The present work was aimed at investigating hydroethanolic leaf extracts of Cassia fistula for their antioxidant and pancreatic lipase (PL) enzyme inhibitory properties. The most active extract was selected to profile the phytoconstituents by UHPLC-QTOF-MS/MS technique. Among the tested extracts, the 80% hydroethanolic extract exhibited the maximum levels of total phenolic and flavonoid contents (TPC and TFC) with a contribution of 201.3 ± 2.6 mg of gallic acid equivalent per gram of extract (GAE/g extract), and 116.3 ± 2.4 mg of rutin equivalent per gram of extract (RE/g extract), respectively. The same extract also showed promising 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and PL inhibitory activity with an IC50 (half maximal inhibitory concentration) of 30.5 ± 2.8 µg/mL and 17.31 ± 1.18 μg/mL, respectively. The phytochemical profiling of 80% hydroethanolic extract confirmed the presence of 23 metabolites of immense medicinal significance. Docking studies were conducted to investigate the potential interactions of compounds identified in the study. The docking study-based binding energy data and the interaction scheme both revealed the possible role of the identified compounds towards PL inhibitor. Moreover, energies of frontier molecular orbitals (FMOs), ionization potentials (IP), electron affinities (EA) and molecular electrostatic potentials (MEP) were also explored. The findings of the current work suggest that C. fistula is a promising natural source of antioxidant and antiobesity agents, which may be exploited to add pharmacological functionalities to food.

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Ultrafiltration LC-ESI-MSn screening of 5-lipoxygenase inhibitors from selected Chinese medicinal herbs Saposhnikovia divaricata, Smilax glabra, Pueraria lobata and Carthamus tinctorius

Zhao

et al. 2016

Journal of Functional Foods

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PREPARATIVE SEPARATION OF HYDROXYL SAFFLOWER YELLOW A AND ANHYDROSAFFLOR YELLOW B IN PLANT EXTRACT OF CARTHAMUS TINCTORIUS L. BY REVERSE-PHASE MEDIUM-PRESSURE LIQUID CHROMATOGRAPHY

Cited by 9 publications

References 6 publications

Simultaneous Optimization of the Ultrasonic Extraction Method and Determination of the Antioxidant Activities of Hydroxysafflor Yellow A and Anhydrosafflor Yellow B from Safflower Using a Response Surface Methodology

Simultaneous Optimization of the Ultrasonic Extraction Method and Determination of the Antioxidant Activities of Hydroxysafflor Yellow A and Anhydrosafflor Yellow B from Safflower Using a Response Surface Methodology

Cassia fistula Leaves; UHPLC-QTOF-MS/MS Based Metabolite Profiling and Molecular Docking Insights to Explore Bioactives Role towards Inhibition of Pancreatic Lipase

Ultrafiltration LC-ESI-MSn screening of 5-lipoxygenase inhibitors from selected Chinese medicinal herbs Saposhnikovia divaricata, Smilax glabra, Pueraria lobata and Carthamus tinctorius

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