Background: The Arbaeen march ritual stands as one of the largest congregations, attended by tens of millions every year. Given the large number of participants in this ritual, it is essential to minimize the health repercussions for pilgrims and ensure their prompt access to medical care. The present study was conducted to analyze the healthcare services during the 2019 Arbaeen march. Materials and Methods: Employing a qualitative approach, this study utilized various data collection methods, including in-depth and semi-structured interviews, field observations, and document review. The target population comprised healthcare decision-makers, managers, and providers of the 2019 Arbaeen march. The interview and data collection guide were developed in accordance with the World Health Organization (WHO) guidelines. Data collection continued until data saturation. Directed content analysis was employed to analyze the data. Results: Data saturation was achieved after conducting 30 personal interviews. Out of the challenges and significant factors found in healthcare deliveries during the Imam Hussein (AS) Arbaeen march, 9 main themes and categories were identified: Trauma system, disease surveillance system, health issues, health education and promotion, healthcare coordination and collaboration command in the health system, information and communication management, response planning, support and resource management, and safety and security. Conclusion: This study identified the major healthcare issues in the AS Arbaeen march, serving as an evidence-based guide for policymakers and planners of this famous religious ritual. The results of this study are applicable to similar public gatherings and marches.