Presence and Development with Age of Aminopeptidase(s) in Tears of Children and other Mammals

Watson, Ronald R.

doi:10.1159/000264912

Cited by 5 publications

(1 citation statement)

References 2 publications

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“…We have shown that most of the sulphatase activity is in the soluble fi'action of tears, although a 8.6+2.4 (10) 10.4+2.0 (7) 112+37 (10) 116 +47 (7) 13__+4 (10) 11+3 (7) 0.28+0.06(17) 0.28+0.10(11) 32+10 (15) 28+8 (8) 3.0+ 1.3 (10) 3.2+ 1.6 (7) small proportion of the activity is associated with particulate material. Watson (1977) has suggested that a number of tear proteins, including albumin, immunoglobulin G and aminopeptidases, are derived from serum because their levels are unaltered in malnutrition while other tear proteins, such as secretory immunoglobulin A, lysozylne and amylase, which are synthesized at mucosal surfaces, are considerably depressed during malnutrition. We have observed considerable interindividual variation in tear weights, tear protein concentration and enzyme activities, but we have not investigated the physiological basis of this variation.…”

Section: Discussionmentioning

confidence: 99%

The 4‐methylumbelliferone sulphate sulphatases of human tears

Jordan

Heiss

Monk

et al. 1979

J of Inher Metab Disea

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The properties of human tear 4-methylumbelliferone sulphate sulphatase (EC 3.1.6.1) have been investigated. More than 80% of the enzyme activity behaved as the acidic A isoenzyme on isoelectric focusing and DEAE-cellulose ion exchange chromatography. The distribution of enzyme activity in a normal population has been investigated. The least interindividual variation was seen when enzyme activities were calculated as units per mg tears. The alpha-galactosidase, beta-N-acetylglucosaminidase and sulphatase enzyme activities in human tears varied independently of each other. The use of tear sulphatases for the detection of metachromatic leukodystrophy (McKusick 24980) is discussed.

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Section: Discussionmentioning

confidence: 99%

The 4‐methylumbelliferone sulphate sulphatases of human tears

Jordan

Heiss

Monk

et al. 1979

J of Inher Metab Disea

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Effect of age, malnutrition and renutrition on free secretory component and IgA in secretions

Watson¹,

McMurray²,

Martin³

et al. 1985

The American Journal of Clinical Nutrition

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The development of free secretory component (FSC) was studied in the tears of normal infants, children and adults. The level of FSC in tears was higher in older adults than in children. Free secretory component was also measured in the tears of normal, moderately and severely malnourished Colombian children. Children suffering from kwashiorkor, combined protein-calorie malnutrition or marasmus were studied before and after renutrition. No change was detected in the concentration of FSC in tears of moderately malnourished (Grade I and II) children. There was a significant difference between normal and severely malnourished children which improved with renutrition. The levels of tear IgA were decreased in the moderately malnourished children. These results indicate that reduction in secretory IgA levels in moderate malnutrition may not be explained by a lack of available free secretory component in tears, but that severe malnutrition may impair the S-IgA system by significantly reducing the availability of free secretory component.

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Effect of Moderate Chronic Protein Deficiency on Rat Salivary Components

Watson¹,

Antal²

1980

The Journal of Nutrition

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Chronic protein insufficiency was achieved in rats with an 8% protein diet initiated at weaning. The total activity and activity per milliliter of the principal salivary protein, amylase, were significantly decreased at day 66. The amount of total protein per milliliter of saliva and total protein in stimulated saliva collected during 30 minutes was decreased in the malnourished rats. The levels of immunoglobulin G (IgG) and aminopeptidase in saliva were not affected by dietary status. Prolonged malnutrition on the low-protein, high-starch diet resulted in an apparent stimulation or induction of salivary amylase and protein secretion by day 110. Total protein collected and milligram of protein per milliliter of saliva were very similar in the malnourished and control rats at day 110. Salivary amylase activity in the malnourished rats was significantly greater than in the saliva of controls. The initial decrease in the principal salivary protein, amylase, and in total protein followed by a significant increase with prolonged stimulation by low-protein and high-starch diets suggests that early protein deficiency retards synthesis of amylase and that a high-starch diet may induce salivary amylase production.

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Presence and Development with Age of Aminopeptidase(s) in Tears of Children and other Mammals

Cited by 5 publications

References 2 publications

The 4‐methylumbelliferone sulphate sulphatases of human tears

The 4‐methylumbelliferone sulphate sulphatases of human tears

Effect of age, malnutrition and renutrition on free secretory component and IgA in secretions

Effect of Moderate Chronic Protein Deficiency on Rat Salivary Components

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