Presence of functional angiotensin II receptor and angiotensin converting enzyme in the aorta of the snake Bothrops jararaca

Esteves, Carlos Augusto; Burckhardt, P.L.; Breno, M.C.

doi:10.1016/j.lfs.2012.09.006

Cited by 6 publications

(12 citation statements)

References 63 publications

(102 reference statements)

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“…Thus, it is possible that the endothelial cells are still at a relatively primitive stage and that ACh induces a contractile response, in the Habu. Ang II-induced contraction has also been shown in other snake species; specifically, Naja naja [ 43 ], Bothrops jararaca [ 9 ], and Pantherophis obsoletus [ 7 ], and the pEC 50 value in the Tokunoshima Habu snakes was similar to that in Naja naja (8.09 vs. 8.00−9.45). These results indicate that the renin-angiotensin system is important for vascular responses in the Habu snake, as in other snake species.…”

mentioning

confidence: 65%

“…Therefore, in this study, we set out to investigate differences in vasoreactivity between snakes of the same species (the Habu) in the Japanese islands of Tokunoshima and Amami-Oshima, using isolated thoracic aortas. We selected this vessel for evaluation because its vasoreactivity had previously been investigated in snakes [ 7 , 9 , 18 , 43 ].…”

mentioning

confidence: 99%

“…We used the following reagents based on previous studies [ 7 , 9 , 18 , 42 , 43 ], at the final concentrations shown in parenthesis: Ang II acetate salt (10 −9 –10 −5 M), NA (10 −9 –10 −4 M), atropine (10 −5 M), methoctramine hydrate (10 −6 M) and sodium nitroprusside (SNP; 10 −4 M; Sigma-Aldrich, St. Louis, MO, USA), ACh chloride (10 −9 –10 −4 M; Daiichi-Sankyo Inc., Tokyo, Japan), pirenzepine dihydrochloride (10 −6 M; Santa Cruz Biotechnology, Santa Cruz, CA, USA), hexahydro-sila-difenidol hydrochloride, p -fluoroanalog (pfHHSiD; 10 −6 M; Research Biochemicals, Natick, MA, USA), and 5-HT (serotonin)-creatinine sulfate (10 −9 –10 −5 M; Merck, Darmstadt, Germany). All reagents were dissolved in distilled water.…”

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confidence: 99%

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Habu snakes (<i>Protobothrops flavoviridis</i>) show variation in thoracic aortic vasoreactivity between adjacent Japanese islands

OOTAWA,

WU,

SEKIO

et al. 2024

The Journal of Veterinary Medical Science

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Habu snakes ( Protobothrops flavoviridis ) are pit vipers found in the geographically adjacent but ecologically divergent islands of Tokunoshima and Amami-Oshima in southwestern Japan. Abiotic factors can cause variation in animal populations between the two islands, and Habu snakes may show such intraspecific physiological variation. We therefore evaluated the vasoreactivity in aortas isolated from the Habu of both islands. Tokunoshima Habu showed significantly greater contractile responses to angiotensin (Ang) II, acetylcholine (ACh) and noradrenaline, and significantly higher affinities (pEC 50 ) for Ang II and ACh, than Amami-Oshima Habu. ACh caused contractions in aortas from both populations, a finding previously unreported in snakes. Our findings indicate that vasoreactivity may differ between Tokunoshima and Amami-Oshima Habu.

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confidence: 65%

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confidence: 99%

mentioning

confidence: 99%

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Habu snakes (<i>Protobothrops flavoviridis</i>) show variation in thoracic aortic vasoreactivity between adjacent Japanese islands

OOTAWA,

WU,

SEKIO

et al. 2024

The Journal of Veterinary Medical Science

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Section: Introductionmentioning

confidence: 99%

“…Vasoreactivity has been studied in the thoracic aorta in three terrestrial snakes [6][7][8][9], spanning the Natricinae, Elapidae, and Viperidae families. Common features across these reports included noradrenaline-induced contractions in two of three species [7,9], angiotensin II-induced contractions in two of three species [6,7], and acetylcholine-induced relaxation in one of three species [8]. Vasoreactivity has been investigated in only one semi-arboreal snake, the yellow rat snake (Pantherophis obsoletus) [10], which showed both some similarities and some differences with the terrestrial snakes.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Vasoreactivity in a Semi-Arboreal Snake, the Tokara Habu (Protobothrops tokarensis)

Ootawa,

Wu,

Sekio

et al. 2023

Animals

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Vasoreactivity is relatively well documented in terrestrial snakes but has previously been investigated in only one semi-arboreal snake species. Consequently, the extent to which vasoreactivity is common across snake taxa or varies by habitat is unclear. The Tokara habu (Protobothrops tokarensis) is a semi-arboreal snake endemic to only two small adjacent Japanese islands, and hence a useful species for further investigation of vasoreactivity. We evaluated responses to known vasoactive substances in thoracic aortas isolated from Tokara habu. Under resting tension, noradrenaline and angiotensin II induced concentration-dependent contraction, but acetylcholine, serotonin (5-hydroxytriptamine; 5-HT), and isoproterenol induced relaxation followed by contraction. Histamine and rattlesnake bradykinin had no effect. Experiments with receptor-specific antagonists suggest that M1 and M3 receptors are involved in the acetylcholine-induced response; 5-HT1, 5-HT2, and 5-HT7 receptors in the serotonin-induced response; and β1 and β2 adrenoceptors in isoproterenol-induced relaxation. This is the first report on such response patterns in snakes (including serotonin- and isoproterenol-induced relaxation). Nitric oxide may be involved in acetylcholine-induced relaxation but not in the responses to serotonin or isoproterenol. In contrast to the uniform vasoreactivity observed in terrestrial snakes, the vasoreactivity of semi-arboreal snakes may be governed by diverse regulatory mechanisms.

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Chronic captopril treatment reveals the role of ANG II in cardiovascular function of embryonic American alligators (Alligator mississippiensis)

et al. 2018

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Angiotensin II (ANG II) is a powerful vasoconstrictor of the renin-angiotensin system (RAS) that plays an important role in cardiovascular regulation in adult and developing vertebrates. Knowledge of ANG II's contribution to developmental cardiovascular function comes from studies in fetal mammals and embryonic chickens. This is the first study to examine the role of ANG II in cardiovascular control in an embryonic reptile, the American alligator (Alligator mississippiensis). Using chronic low (~ 5-mg kg embryo), or high doses (~ 450-mg kg embryo) of captopril, an angiotensin-converting enzyme (ACE) inhibitor, we disrupted the RAS and examined the influence of ANG II in cardiovascular function at 90% of embryonic development. Compared to embryos injected with saline, mean arterial pressure (MAP) was significantly reduced by 41 and 72% under low- and high-dose captopril treatments, respectively, a greater decrease in MAP than observed in other developing vertebrates following ACE inhibition. Acute exogenous ANG II injection produced a stronger hypertensive response in low-dose captopril-treated embryos compared to saline injection embryos. However, ACE inhibition with the low dose of captopril did not change adrenergic tone, and the ANG II response did not include an α-adrenergic component. Despite decreased MAP that caused a left shifted baroreflex curve for low-dose captopril embryos, ANG II did not influence baroreflex sensitivity. This study demonstrates that ANG II contributes to cardiovascular function in a developing reptile, and that the RAS contributes to arterial blood pressure maintenance during development across multiple vertebrate groups.

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Presence of functional angiotensin II receptor and angiotensin converting enzyme in the aorta of the snake Bothrops jararaca

Abstract: The data contribute to the knowledge of the renin-angiotensin system in vertebrate species, and provide insight into the understanding of snake Ang II receptor characteristics and diversity.

Cited by 6 publications

References 63 publications

Habu snakes (<i>Protobothrops flavoviridis</i>) show variation in thoracic aortic vasoreactivity between adjacent Japanese islands

Habu snakes (<i>Protobothrops flavoviridis</i>) show variation in thoracic aortic vasoreactivity between adjacent Japanese islands

Characterization of Vasoreactivity in a Semi-Arboreal Snake, the Tokara Habu (Protobothrops tokarensis)

Chronic captopril treatment reveals the role of ANG II in cardiovascular function of embryonic American alligators (Alligator mississippiensis)

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