Preservation of human artery function following prolonged cold storage with a new solution

Garbe, Stephanie; Zatschler, Birgit; Müller, Bianca; Dieterich, Peter; Ebner, Annette; Rauen, Ursula; Matschke, Klaus; Deußen, Andreas

doi:10.1016/j.jvs.2010.10.093

Cited by 29 publications

(30 citation statements)

References 16 publications

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“…Studies of human thoracic artery (ATI) [4] yield similarly positive results, as does the present study using human veins. For ATI, too, vascular tone was better preserved after four days in the TiProtec solution than in either 0.9% NaCl or the HTK solution.…”

Section: Study Limitationssupporting

confidence: 75%

“…Injury due to cold storage and prevention from injury have already been exhibited for corneal cells, cultured endothelial cells, hepatocytes, rat mesenteric artery, segments of rat and pig aorta and lung epithelial cells [1][2][3]. The same is true for the human internal thoracic artery [4]. On the other hand, preservation solutions themselves can develop an inherent toxicity upon rewarming [5][6][7].…”

Section: Introductionmentioning

confidence: 99%

“…These older solutions include Histidine-Tryptophan-Ketoglutarate (HTK) solution (Custodiol) and University of Wisconsin solution (Viaspan). HTK solution was the basis of the newer Tissue Protection (TiProtec) solution that was specially developed [4] for the preservation of blood vessels and therefore contains iron chelators.…”

Section: Introductionmentioning

confidence: 99%

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Function and Viability of Vessels in Different Preservation Solutions - An Experimental Study on Human Great Saphenous Veins

Schipke¹

2016

AVS

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ObjectiveGreat Saphenous Veins (SVs) are widely used in coronary and in non-coronary surgery, and the need for vascular transplants is increasing. In older pre¬servation solutions, hypothermia leads to damage induced by reactive oxygen species. Iron chelators in the newer Tissue Protection (TiProtec) solution shall par¬ticularly protect vessels by preventing from that damage.It was the aim to compare three preservation solutions to maintain function and viability of human SVs. MethodsVein segments were randomly assigned to Krebs-Henseleit Buffer (KHB), Viaspan (UW), or TiProtec. Contraction/relaxation in organ baths was assessed at 3, 24, 48 and 72h after removal. The protocol included three steps: receptor-independent contraction (KCl: 80μM), receptor-dependent contraction (PE: ≤30μM), endothelium-dependent relaxation (ACh: ≤20μM). Morphology: Vein segments were incubated with propidium iodide and 5-chloromethylfluorescein diacetate. Viability was assessed with fluorescence using laser scanning microscopy. ResultsKHB was inappropriate after only 3h. In TiProtec, SVs contraction/relaxation had increased further at 72h, but not in both other groups. More necrosis was found in KHB SVs than in UW or TiProtec at 3h. More viable cells were found in UW and in TiProtec SVs on day-1, while only few cells were viable in KHB on day-10. Summary and conclusionEven short storage of SVs in KHB is deleterious. UW preserved func¬tion better over time. The best functional and morphological results yielded TiProtec over time, and the amount of viable cells correlated with function. TiProtec seems a promising alternative for short-and mid-term hypothermic vein preservation.

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Section: Study Limitationssupporting

confidence: 75%

Section: Introductionmentioning

confidence: 99%

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Function and Viability of Vessels in Different Preservation Solutions - An Experimental Study on Human Great Saphenous Veins

Schipke¹

2016

AVS

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“…Tryptophan is commonly included in the conservation medium for graft vessels, and it may be necessary for vascular protection. 30 However, its precise role is not completely understood to date. Taurine is a free amino acid, many of which exist in vivo, and it is known that it has various physiological effects.…”

Section: Discussionmentioning

confidence: 99%

Identification of Biomarkers of Stent Restenosis With Serum Metabolomic Profiling Using Gas Chromatography/Mass Spectrometry

Hasokawa

Shinohara

Tsugawa

et al. 2012

Circ J

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“…13 TiProtec contains iron chelators to inhibit cold-induced iron-dependent cell injury, 14,15 glycine and alanine to prevent hypoxic injury, 16,17 and high chloride and potassium concentrations, which both proved favorable for cold storage of vessels. 10,11 …”

Section: Introductionmentioning

confidence: 99%

Serum- and albumin-free cryopreservation of endothelial monolayers with a new solution

2018

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Cryopreservation is the only long-term storage option for the storage of vessels and vascular constructs. However, endothelial barrier function is almost completely lost after cryopreservation in most established cryopreservation solutions. We here aimed to improve endothelial function after cryopreservation using the 2D-model of porcine aortic endothelial cell monolayers. The monolayers were cryopreserved in cell culture medium or cold storage solutions based on the 4°C vascular preservation solution TiProtec®, all supplemented with 10% DMSO, using different temperature gradients. After short-term storage at −80°C, monolayers were rapidly thawed and re-cultured in cell culture medium. Thawing after cryopreservation in cell culture medium caused both immediate and delayed cell death, resulting in 11 ± 5% living cells after 24 h of re-culture. After cryopreservation in TiProtec and chloride-poor modifications thereof, the proportion of adherent viable cells was markedly increased compared to cryopreservation in cell culture medium (TiProtec: 38 ± 11%, modified TiProtec solutions ≥ 50%). Using these solutions, cells cryopreserved in a sub-confluent state were able to proliferate during re-culture. Mitochondrial fragmentation was observed in all solutions, but was partially reversible after cryopreservation in TiProtec and almost completely reversible in modified solutions within 3 h of re-culture. The superior protection of TiProtec and its modifications was apparent at all temperature gradients; however, best results were achieved with a cooling rate of −1°C/min. In conclusion, the use of TiProtec or modifications thereof as base solution for cryopreservation greatly improved cryopreservation results for endothelial monolayers in terms of survival and of monolayer and mitochondrial integrity.

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Preservation of human artery function following prolonged cold storage with a new solution

Abstract: In contrast to traditional HTK, NaCl, or PSS storage, TiProtec solution offers an excellent potential for prolonged cold storage of human arteries, which may close the existing gap between legal requirements for tissue banking and current cold preservation methods.

Cited by 29 publications

References 16 publications

Function and Viability of Vessels in Different Preservation Solutions - An Experimental Study on Human Great Saphenous Veins

Function and Viability of Vessels in Different Preservation Solutions - An Experimental Study on Human Great Saphenous Veins

Identification of Biomarkers of Stent Restenosis With Serum Metabolomic Profiling Using Gas Chromatography/Mass Spectrometry

Serum- and albumin-free cryopreservation of endothelial monolayers with a new solution

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