Pressure‐assisted capillary electrochromatography with electrospray ionization‐mass spectrometry based on silica‐based monolithic column for rapid analysis of narcotics

Abstract

A pressure-assisted CEC (pCEC) with ESI-MS based on silica-based monolithic column was developed for rapid analysis of narcotics. Combining the extremely high permeability and separation efficiency of silica-based monolithic column with the high selectivity and sensitivity of pCEC-ESI-MS, the developed system exhibited its prominent advantages in separation and detection. A systematic investigation of the pCEC separation and ESI-MS detection parameters was performed. Experiment results showed that the optimized separation efficiency could be obtained at 8 bar assisted pressure with 25 kV separation voltage, using the solution containing 65% ACN v/v and 20 mmol/L ammonium acetate with pH 6.0 as running buffer. 3 microL/min of sheath liquid was considered as the optimized flow rate since it could provide the maximum signal intensity. Under the optimum conditions, the tested five narcotics could be completely separated within 10 min with the detection limit in the range of 2.0-80 nmol/L. The proposed method has been successfully used for detection of narcotics in real urine samples.

Rapid analysis of peptides and amino acids by CE‐ESI‐MS using chemically modified fused‐silica capillaries

“…1. First, a rehydroxylation process to maximize the number of silanol groups of the Electrophoresis 2009Electrophoresis , 30, 2273Electrophoresis -2279 bare-FS capillary was performed as described previously [49]. In brief, the capillary was flushed with water, 1.0 mol/L sodium hydroxide, water, 0.1 mol/L hydrochloric acid, water, and acetone for 0.5, 3, 0.5, 3, 0.5, and 0.5 h in order, respectively.…”

CE-MS in Forensic Sciences with Focus on Forensic Toxicology

“…A peculiar variant of CE, named pressure-assisted CEC (pCEC) with ESI-MS (single quadrupole) using a silica-based monolithic column, was successfully applied by Lu et al and for rapid analysis of five typical narcotics such as methadone, pethidine, fentanyl, morphine, and diamorphine (i.e., medicinal heroin) in real urine samples [106]. The proposed method showed distinct advantageous features, such as high sensitivity, speed, high efficiency, low sample and organic solvent consumption.…”

On‐line concentration and pressurized capillary electrochromatography analysis of five β‐agonists in human urine using a methacrylate monolithic column

“…Furthermore, methacrylate ester‐based monolithic columns have been prepared with desired monomers and cross‐linking agent together with suitable porogenic solvents in CEC. Recently, our group has developed CEC methods for simultaneous determination of β 2 ‐agonists in urine samples . However, CEC is easy to produce the problem such as bubbles and dry column.…”