Pressure effects on the interactions of the sarcoplasmic reticulum calcium transport enzyme with calcium and para-nitrophenyl phosphate

Hasselbach, Wilhelm; Stephan, Lore

doi:10.1016/s0022-2828(87)80757-5

Cited by 3 publications

(8 citation statements)

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“…As cytoskeletal microtubules appear to influence BK channel gating (Kitzmiller and Rosenberg, 1997), high pressure might conceivably act by the same route. A -AV of 105 ml mol-1 is also consistent with the dissociation of a ligand, perhaps an endogenous blocker (Methfessel and Boheim, 1982), or with conformational changes in proteins (Jonas and Jonas, 1994;Gross and Jaenicke, 1994;Weber, 1993;Van Eldik et al, 1989;Hasselbach and Stephan, 1987). Finally, as the mobility of ions in aqueous solution is not affected by the pressure used here (Brummer and Hills, 1961), and as the BK channel conductance is not affected by 900 atm (like alamethicin channels at 1000 atm; Brunner and Hall, 1983), there is no reason to suppose that the geometry of the BK channel pore is affected by pressure.…”

Section: Figure 3 Effect Of Pressure On the Channel Open Probabilitymentioning

confidence: 64%

“…The highest pressure used here has a negligible effect on the pH of Tris buffer (Disteche, 1972). The Ca + EGTA -CaEGTA association reaction involves a AV of +20.4 ml mol-1 (Hasselbach and Stephan, 1987), and accordingly its equilibrium constant is halved by 900 atm, doubling the free calcium concentration. At the negligible level used here…”

Section: Figure 3 Effect Of Pressure On the Channel Open Probabilitymentioning

confidence: 99%

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Effect of high hydrostatic pressure on the BK channel in bovine chromaffin cells

Macdonald

1997

Biophysical Journal

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The activity of the BK channel of bovine chromaffin cells was studied at high hydrostatic pressure, using inside-out patches in symmetrical KCl solution, Ca2+-free and at V(H) = -60 to -40 mV. Pressure increased the probability of channels being open (900 atm increasing the probability 30-fold), and it increased the minimum number of channels apparent in the patches. The pressure activation of the channel was reversed on decompression. Channel conductance was unaffected. It was shown that pressure did not act by raising the temperature, or by affecting [Ca] or pH, or the order of the membrane bilayer, and it was concluded that pressure most likely acted directly on the channel proteins and/or their modulating reactions.

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Section: Figure 3 Effect Of Pressure On the Channel Open Probabilitymentioning

confidence: 64%

Section: Figure 3 Effect Of Pressure On the Channel Open Probabilitymentioning

confidence: 99%

Effect of high hydrostatic pressure on the BK channel in bovine chromaffin cells

Macdonald

1997

Biophysical Journal

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“…The binding reaction of a buffer to a ligand is influenced by environmental conditions such as temperature, pH and pressure. As the pH of any solution typically changes with pressure, hydrostatic pressure affects the free calcium ion concentration both directly [ 15 ] and through a change in pH [ 9 ].…”

Section: Methodsmentioning

confidence: 99%

“… Pressure-dependence of pH due to pressure effects on HEPES according to Eq (14) [ 17 ] using the Δ i v determined by Kitamura et al [ 16 ]. The development of the equilibrium constant of EGTA binding to Ca 2+ is estimated by Eq (16) using the Δ r v determined by Hasselbach et al [ 15 ]. …”

Section: Methodsmentioning

confidence: 99%

“…The equilibrium constants were taken from the NIST database [ 19 ]. The equilibrium reaction of the binding of calcium to the calcium buffer EGTA has a molar reaction volume of Δ r v of 20.4 ml mol -1 [ 15 ] so that the reaction equilibrium is expected to be shifted towards releasing more free calcium ions under high hydrostatic pressure (i.e. decreasing EGTA binding affinity to Ca 2+ ).…”

Section: Methodsmentioning

confidence: 99%

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Calcium Sensitive Fluorescent Dyes Fluo-4 and Fura Red under Pressure: Behaviour of Fluorescence and Buffer Properties under Hydrostatic Pressures up to 200 MPa

et al. 2016

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The fluorescent Ca2+ sensitive dyes Fura Red (ratiometric) and Fluo-4 (non-ratiometric) are widely utilized for the optical assessment of Ca2+ fluctuations in vitro as well as in situ. The fluorescent behavior of these dyes is strongly depends on temperature, pH, ionic strength and pressure. It is crucial to understand the response of these dyes to pressure when applying calcium imaging technologies in the field of high pressure bioscience. Therefore, we use an optically accessible pressure vessel to pressurize physiological Ca2+-buffered solutions at different fixed concentrations of free Ca2+ (1 nM to 25.6 μM) and a specified dye concentration (12 μM) to pressures of 200 MPa, and record dye fluorescence intensity. Our results show that Fluo-4 fluorescence intensity is reduced by 31% per 100 MPa, the intensity of Fura Red is reduced by 10% per 100 MPa. The mean reaction volume for the dissociation of calcium from the dye molecules is determined to -17.8 ml mol-1 for Fluo-4 and -21.3 ml mol-1 for Fura Red. Additionally, a model is presented that is used to correct for pressure-dependent changes in pH and binding affinity of Ca2+ to EGTA, as well as to determine the influence of these changes on dye fluorescence.

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Fluorescent probe dyes for metabolic‐ion sensing under high hydrostatic pressures

Urayama

Frey

Savage

2010

Annals of the New York Academy of Sciences

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Fluorescence spectroscopy and microscopy imaging are widely used at ambient pressure for analytical studies on biological systems. Before using fluorescence-based methods at high pressures, biochemical and metabolic probes need to be characterized under pressure to ensure valid quantitative results. In this review, we describe the principles behind the use of fluorescent probe dyes for ion sensing and provide models for interpreting the dye spectrum under pressure. We then review results from three studies using the excited-state emission from probe dyes sensitive to pH and calcium-ion concentration, demonstrating some ways pressure may affect probe operation.

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Pressure effects on the interactions of the sarcoplasmic reticulum calcium transport enzyme with calcium and para-nitrophenyl phosphate

Cited by 3 publications

References 0 publications

Effect of high hydrostatic pressure on the BK channel in bovine chromaffin cells

Effect of high hydrostatic pressure on the BK channel in bovine chromaffin cells

Calcium Sensitive Fluorescent Dyes Fluo-4 and Fura Red under Pressure: Behaviour of Fluorescence and Buffer Properties under Hydrostatic Pressures up to 200 MPa

Fluorescent probe dyes for metabolic‐ion sensing under high hydrostatic pressures

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