Pressure measurement methods in microchannels: advances and applications

Shen, Feng; Ai, Mingzhu; Li, Zonghe; Lu, Xinran; Pang, Yan; Liu, Zhaomiao

doi:10.1007/s10404-021-02435-w

Cited by 11 publications

(1 citation statement)

References 129 publications

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“…To overcome these problems, innovative miniaturized technologies for detecting pressure changes in LoCs are currently under study [2]. As common approach, the pressureinduced deformation of a membrane integrated to a LoC is detected using a variety of electrical, piezoresistive and optical methods [3][4][5][6][7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Catalase Detection via Membrane-Based Pressure Sensors

Bianco,

Zizzari,

Perrone

et al. 2024

Molecules

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Membrane-based sensors (MePSs) exhibit remarkable precision and sensitivity in detecting pressure changes. MePSs are commonly used to monitor catalytic reactions in solution, generating gas products crucial for signal amplification in bioassays. They also allow for catalyst quantification by indirectly measuring the pressure generated by the gaseous products. This is particularly interesting for detecting enzymes in biofluids associated with disease onset. To enhance the performance of a MePS, various structural factors influence membrane flexibility and response time, ultimately dictating the device’s pressure sensitivity. In this study, we fabricated MePSs using polydimethylsiloxane (PDMS) and investigated how structural modifications affect the Young’s modulus (E) and residual stress (σ0) of the membranes. These modifications have a direct impact on the sensors’ sensitivity to pressure variations, observed as a function of the volume of the chamber (Σ) or of the mechanical properties of the membrane itself (S). MePSs exhibiting the highest sensitivities were then employed to detect catalyst quantities inducing the dismutation of hydrogen peroxide, producing dioxygen as a gaseous product. As a result, a catalase enzyme was successfully detected using these optimized MePSs, achieving a remarkable sensitivity of (22.7 ± 1.2) µm/nM and a limit of detection (LoD) of 396 pM.

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