Pressure overload changes mesenteric afferent nerve responses in a stress-dependent way in a fasting rat model

Bao, Lingxia; Zhao, Jingbo; Liao, Donghua; Wang, Guixue; Gregersen, Hans

doi:10.1007/s10237-020-01305-8

Cited by 3 publications

(2 citation statements)

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“…Our previous studies in fasting rats demonstrated intestinal histomorphological and biomechanical remodeling (Dou et al 2002) and increased mechanosensitivity of intestinal afferents (Bao et al 2019(Bao et al , 2020. However, those studies did not combine afferent nerve recordings with mechanical and histological data.…”

Section: Discussionmentioning

confidence: 97%

“…In this study, we hypothesized that the fasting-induced hypersensitivity of the small intestinal afferents to mechanical stimulation was closely associated with the fasting-induced intestinal remodeling. Based on previous studies on intestinal stresses and strains (Bao et al 2020), we speci cally hypothesized that remodeling of sensitivity depends on stress. Therefore, in the present study, we aimed to explore the relationship between jejunal remodeling and the intestinal afferent sensitivity by combining afferent nerve recordings with histology and mechanical data.…”

Section: Introductionmentioning

confidence: 99%

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Association between jejunal remodeling in fasting rats and hypersensitivity of intestinal afferent nerves to mechanical stimulation

Bao

Zhao

Gregersen

2023

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Remodeling of Intestinal properties and hypersensitivity of intestinal afferents to mechanical stimulation were previously demonstrated in a fasting rat model. Other studies investigated the association between mechanical and histological remodeling during fasting. This study aimed to further explore the relationship between the jejunal remodeling and intestinal afferent hypersensitivity by combining afferent nerve recordings with histological and mechanical data. Eight male Sprague Dawley rats had no access to food for 7 days (Fasting group). Seven male rats served as controls (Control group). Jejunal segments were studied in vitro in an organ bath for analysis of afferent signaling and for analysis of mechanical and histomorphological parameters. Correlation analyses were done to analyze association between nerve activity (spike rate increase ratio, SRIR) at distension levels of 20, 40 and 80 cmH2O and mechanical stress and histomorphological changes of the jejunal segments. Compared with the Control group, the main findings in jejunal segments in the Fasting group were 1) Most histomorphometry parameters were reduced (P<0.05-P<0.001), 2) SRIR values were higher (P<0.001), 3) The relative numbers of intermuscular and submucosal neurons were increased (P<0.05- P<0.01), and 4) SRIR was negatively correlated with intestinal wall thickness, circumferential muscle layer thickness and positively correlated with the inner residual strain, the number of neurons, and the mechanical stress. In conclusion, the fasting-induced histomorphological remodeling (reduced wall thickness and increased relative number of neurons) and biomechanical remodeling (residual strain changes and high stress level) of the intestine in fasting rats were associated with hypersensitivity of intestinal afferents. Afferent hypersensitivity appears to be dependent on stress rather than on strain.

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Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%