Pressurized, annular chromatograph for continuous separations

Scott, Charles D.; Spence, R. M.; Sisson, Warren G.

doi:10.1016/s0021-9673(01)84087-5

Cited by 65 publications

(24 citation statements)

References 6 publications

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“…They observed little improvement in throughput over the gravity fed units, because of the controlled rate of the multistreams. Scott and coworkers [10] were the first group to assemble a continuous annular chromatograph (CAC) capable of pressurized operation. They closed the two concentric cylinders on top, which formed a headspace, and provided a gas blanket over the annulus (Fig.…”

Section: Development Of Continuous Annular Chromatographymentioning

confidence: 99%

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Preparative continuous annular chromatography (P-CAC), a review

Uretschläger

Jungbauer

2002

Bioprocess and Biosystems Engineering

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Notation c liquid phase solute concentration (g/l) c* liquid concentration in equilibrium (g/l) D z axial dispersion coefficient (cm 2 /min) D m solute dispersion coefficient in the axial direction (cm 2 /min) D h solute dispersion coefficient in the angular direction (cm 2 /min) k 0 a global mass transfer parameter (s -1 ) k¢ A normalized retention of compound A k¢ B normalized retention of compound B K distribution coefficient K m mass transfer coefficient between the mobile and the stationary phases K eq equilibrium partition coefficient m p amount of pure protein (mg) n cycle factor N eff effective plate number N th number of theoretical plates P productivity (mg/(ml min)) q solid phase solute concentration (g/l) q* solid concentration in equilibrium (g/l) Q F feed flow rate (ml/min) Q E eluent flow rate (ml/min) Q T total flow rate (ml/min) Q quantity Q R recovery ratio R resolution S particle area per unit column volume (cm 2 /ml) t retention time (min) t max reduced retention time (min) t t max retention time of dextran blue (min) t F feed time (min) t c cycle time (min) t c,1 time for the first cycle of batch column (min) t c,b time for n cycles of batch column (min) t¢ c,1 time for the first cycle of P-CAC (min) t¢ c,c time for n cycles of P-CAC (min) u superficial velocity (cm/min) V B buffer consumption V t column volume (ml) V E elution volume (ml)

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Section: Development Of Continuous Annular Chromatographymentioning

confidence: 99%

“…In contrast to Scott and his co-workers [10], Dalvie et al [22] considered mass transfer rates in addition to plate theory. The rate theory approach provides a more precise description of the mass transfer phenomena occurring in a chromatographic column and is therefore more useful for predictive studies.…”

Section: Feed Widthmentioning

confidence: 99%

Preparative continuous annular chromatography (P-CAC), a review

Uretschläger

Jungbauer

2002

Bioprocess and Biosystems Engineering

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“…Plate theory, as modified by Scott et al (1976) to apply to a CAC, will be discussed first. This discussion will be followed by the analytical solution of Rhee et al (1970) to the idealized partial differential equations.…”

Section: Theorymentioning

confidence: 99%

“…Finally, an extension to this solution to include dispersion effects will be presented. Scott et al (1976) mathematically described a CAC by a theoretical-plate approach similar to that developed for conventional chromatographic columns. After the dimensional translation from time to angular position has been made, as follows:…”

Section: Theorymentioning

confidence: 99%

A rotating annular chromatograph for continuous separations

Begovich

Sisson

1984

AIChE Journal

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Preparative, multicomponent liquid chromatographic separations have been achieved by using a slowly rotating annular sorbent bed with fixed multiple feed points and fixed product withdrawal locations. The cation exchange separation of copper, nickel, and two cobalt complexes has been extensively used to study the effects of rotation rate, eluent rate, bed loading, and column size on separation performance. Experimental results have been compared with three theoretical models: plate theory, an analytical solution which incorporates multicomponent Langmuir isotherms, and an extension of the analytical solution to include dispersion effects. SCOPEWith its versatility, high resolution capabilities, and nearuniversal potential applicability, chromatography lacks only throughput capacity to make it an ultimate separation technique. Typical chromatographic separations are made in fixed columns and, as such, are inherently batch in nature. Various attempts have been made to increase the capacity of chromatographic devices either through cyclic operation of large-diameter columns or through continuous feeding of a mixture and continuous removal of the components in moving-bed systems. Several reviews of methods which attempt to achieve continuous chromatographic separations have appeared in the literature, e.g., Rendell (1975), Sussman and Rathore (1975), and Sussman (1976). These include counterflow, moving-bed systems, crossflow moving-bed systems, and simulated moving-bed systems.The objective of this work was to describe the performance results obtained by using a rotating annular sorbent bed, which has been successfully used to achieve continuous separations. The effects of feed rate, eluent velocity, bed loading, and rotation rate on the separation performance of such a device are discussed. Also discussed is the application of plate theory and an extension of the analytical solution describing a rotating annular sorbent bed. With such information, engineers should be able to make large-scale continuous chromatographic separations possible. CONCLUSIONS AND SIGNIFICANCEThis study was made to obtain experimental data showing the effects of feed rate, feed concentration, rotation rate, and eluent velocity on the separation performance of a rotating annular sorbent bed. This bed receives a constant flow of a feed mixture and continuously separates the mixture into its respective solutes.Both plate theory and an extension to the analytical solution of Rhee et al. (1970) to include dispersion effects were applied to the rotating bed. While plate theory is simpler to apply, the extended model is particularly useful when a nonlinear adsorption isotherm must be considered. Agreement between the extended model and experimental concentration profiles was excellent. When a conventional column was used to obtain the necessary adsorption and dispersion parameters of a desired separation, both models adequately predicted the experimental effects of varying the rotation rate and eluent velocity. The extended model, however, with i...

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“…A widely applied continuous system is counter-current simulated moving bed chromatography (Thiele et al, 2001). A true moving bed system is annular bed chromatography, a cross-current axial flow apparatus, which has also been used for a wide range of applications (Fox et al, 1969;Scott et al, 1976;Bloomingburg and Carta, 1994;Bart et al, 1996;Giovannini and Freitag, 2002).…”

Section: Introductionmentioning

confidence: 99%

Continuous Radial Flow Chromatography of Proteins

Lay

Fee

Swan

2006

Food and Bioproducts Processing

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A continuous radial flow chromatography (CRFC) system consisting of an annular packed bed between two rotating concentric porous stainless steel cylinders was constructed and tested. The protein feed, wash buffer and elution buffer are applied simultaneously at different fixed angular positions at the periphery of the rotating bed and flow radially inwards. The target protein is bound in the feed zone and eluted in the elution zone while unbound protein flows through the feed zone. Continuity equations for fixed bed radial flow chromatography were extended to model the CRFC by including angular displacement terms, and solved using finite difference methods. Film diffusion at the resin particle boundary and pore diffusion within the resin particle was described by an overall mass transfer coefficient and a multicomponent Langmuir isotherm was used to describe protein absorption onto the resin particle. To verify the model, a solution containing 0.53 mg ml 21 lactoferrin and 1.6 mg ml 21 bovine serum albumin (BSA) was applied at 5 ml min 21 to a 220-ml CRFC system packed with DEAE Sepharose Fast Flow ion exchange resin, with abed speed of 0.02 rpm. The proteins were separated into lactoferrin of 68% purity (74% recovery) and BSA of 94% purity (85% recovery). Experimental data were used to find values for the parameters in the model proposed.

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Pressurized, annular chromatograph for continuous separations

Cited by 65 publications

References 6 publications

Preparative continuous annular chromatography (P-CAC), a review

Preparative continuous annular chromatography (P-CAC), a review

A rotating annular chromatograph for continuous separations

Continuous Radial Flow Chromatography of Proteins

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