Preterm Birth and Healthy Outcomes Team: the science and strategy of team-based investigation

Tough, Suzanne

doi:10.1186/1471-2393-13-s1-s1

Cited by 3 publications

(1 citation statement)

References 11 publications

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“…Preterm birth is a major societal and economic problem that affects approximately 9.6% of pregnancies worldwide and accounts for 80–90% of neonatal morbidity and death [ 1 – 4 ]. The prevention of preterm birth continues to be an important health priority.…”

Section: Introductionmentioning

confidence: 99%

Modulation of Progesterone Receptor Isoform Expression in Pregnant Human Myometrium

Ilicic

Zakár

Paul

2017

BioMed Research International

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Background. Regulation of myometrial progesterone receptor (PR) expression is an unresolved issue central to understanding the mechanism of functional progesterone withdrawal and initiation of labor in women. Objectives. To determine whether pregnant human myometrium undergoes culture-induced changes in PR isoform expression ex situ and, further, to determine if conditions approaching the in vivo environment stabilise PR isoform expression in culture. Methods. Term nonlaboring human myometrial tissues were cultured under specific conditions: serum supplementation, steroids, stretch, cAMP, PMA, PGF2α, NF-κB inhibitors, or TSA. Following 48 h culture, PR-T, PR-A, and PR-B mRNA levels were determined using qRT-PCR. PR-A/PR-B ratios were calculated. Results. PR-T and PR-A expression and the PR-A/PR-B ratio significantly increased in culture. Steroids prevented the culture-induced increase in PR-T and PR-A expression. Stretch blocked the effects of steroids on PR-T and PR-A expression. PMA further increased the PR-A/PR-B ratio, while TSA blocked culture-induced increases of PR-A expression and the PR-A/PR-B ratio. Conclusion. Human myometrial tissue in culture undergoes changes in PR gene expression consistent with transition toward a laboring phenotype. TSA maintained the nonlaboring PR isoform expression pattern. This suggests that preserving histone and/or nonhistone protein acetylation is critical for maintaining the progesterone dependent quiescent phenotype of human myometrium in culture.

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Section: Introductionmentioning

confidence: 99%

Modulation of Progesterone Receptor Isoform Expression in Pregnant Human Myometrium

Ilicic

Zakár

Paul

2017

BioMed Research International

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Ethics of Predicting and Preventing Preterm Birth

Burke,

Trinidad,

Blacksher

2024

Clinics in Perinatology

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Optineurin suppression activates the mediators involved in the terminal effector pathways of human labour and delivery

Lim

Barker

Lappas

2017

Reprod. Fertil. Dev.

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Spontaneous preterm birth remains the major cause of neonatal death and morbidity. Studies in non-gestational tissues report that optineurin (OPTN) is critical in the termination of NFKB1 activity and control of inflammation, central features of spontaneous preterm birth. The aims of the present study were to determine: (1) OPTN expression in fetal membranes and the myometrium during labour; (2) the effects of IL1B on OPTN expression in primary myometrial cells; and (3) the effects of OPTN short interference (si) RNA on IL1B-stimulated proinflammatory and prolabour mediators. OPTN mRNA and protein expression was significantly decreased with spontaneous term labour in fetal membranes and the myometrium. Although there was no effect of spontaneous preterm labour on OPTN expression in fetal membranes, there was decreased OPTN expression in membranes with chorioamnionitis and myometrial cells treated with 1ng mL IL1B for 1 or 6h. In cells transfected with OPTN siRNA, significant increases were seen in IL1B-stimulated IL6, tumour necrosis factor, CXCL8 and monocyte chemoattractant protein-1 mRNA expression and release, cyclo-oxygenase-2 and prostanoid PTGFR receptor mRNA expression and the release of prostaglandin F. There was no change in IL1B-stimulated NFKBIA expression; however, there was increased NFKB1 p65 DNA-binding activity. The results of the present study suggest that OPTN is a negative regulator of inflammation-induced prolabour mediators.

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Preterm Birth and Healthy Outcomes Team: the science and strategy of team-based investigation

Cited by 3 publications

References 11 publications

Modulation of Progesterone Receptor Isoform Expression in Pregnant Human Myometrium

Modulation of Progesterone Receptor Isoform Expression in Pregnant Human Myometrium

Ethics of Predicting and Preventing Preterm Birth

Optineurin suppression activates the mediators involved in the terminal effector pathways of human labour and delivery

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