Pretreatment free of 2,4-dichlorophenoxyacetic acid improves the differentiation of sugarcane somatic embryos by affecting the hormonal balance and the accumulation of reserves

Reis, Robert L.; Vale, Ellen Moura; Sousa, Kariane Rodrigues de; Santa–Catarina, Claudete; Silveira, Vanildo

doi:10.1007/s11240-020-01995-z

Cited by 36 publications

(15 citation statements)

References 61 publications

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“…Compared to other auxins, hypotheses developed to explain the positive action of 2, 4-D that could be due to inhibition of the DRT102 protein, which is responsible for stimulating cell division, DNA replication and lead to decrease in the number of cells (Pasternak et al 2002). Moreover, in previous study of (Zuo et al 2002), he explained the effect of the hormone 2,4-D as a signal trigged to alert the beginning of hyperpolarization process of membrane polypeptides in in the cell .. Reis et al (2021) recently mentioned that the growth of callus effectively in the arti cial media is linked to 2,4-D hormone homeostasis , which directly effects on the e cient accumulation of storage reserves of callus. On the other hand, the effect of the exogenously applied of 2,4-D and BA may be due to its stimulus to increase the endogenous levels of IAA, which is known to be an activator of cell division and differentiation, especially under dark incubation or low light intensity Migual et al (1996).…”

Section: Resultsmentioning

confidence: 99%

Creation of Borer Pests Resistance Genetically Engineering Peach (Prunus Persica L.) Plants by Cloning cry1Ab Gene

Kadasa

Metwali

Soliman

et al. 2021

Preprint

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The plasmid pBI121 cry1Ab was used to transform peach explants to produce insect resistant plants. The plasmid was constructed from cloning the synthetic cryIAb gene with intron of castor bean catalase-1 gene into the pBI121 binary vector under the control of CaMV35S promoter. Leaf discs of peach (Prunus Persica L.) were co-cultivated for two days with A. tumefaciens strain LBA 4404. Explants were plated on WPM medium supplemented with 125 mg/l kanamycin, 3% sucrose, 2.5 mg L-1 2,4-D (2,4-Dichlorophenoxyacetic acid) and 0.5 mg/l BA (6-benzyladenine) in darkness for callus formation. The calli were then selected on WPM medium supplemented with 125 mg L-1 kanamycin, 3% sucrose, 3.00 mg L-1 TDZ (Thidiazuron), 1.0 mg L-1 Kn (kinetin) and 0.5 mg L-1 IAA (Indoleacetic acid) in the light for at least five subcultures (with a regeneration efficiency of 91.8%). The integration of cryIAb gene into the peach genome was confirmed by PCR (polymerase chain reaction) and northern blot analysis. The transformation efficiency (28%) was obtained when leaves were incubated for 15 min. with A. tumefaciens. The cryIAb gene expression was confirmed using RT-PCR, northern blot hybridization, immune-strip test and insect bioassays, respectively. For insect bioassay, it was evident from data the toxin CryIAb protein expressed in transformed peach plants showed 100% mortality at 1000 ppm against Synanthedon exitiosa larvae’s after 96 hr. These results obtained improved significantly of CryIAb toxin protein against lepidopteron larvae of peach.

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Section: Resultsmentioning

confidence: 99%

Creation of Borer Pests Resistance Genetically Engineering Peach (Prunus Persica L.) Plants by Cloning cry1Ab Gene

Kadasa

Metwali

Soliman

et al. 2021

Preprint

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“…After protein precipitation, the samples were resuspended in 7 M urea/2 M thiourea solution. Aliquots of 100 µg of protein were subjected to tryptic digestion using the lter-aided sample preparation (FASP) methodology (Reis et al 2021). Next, the peptides were resuspended in 100 μL solution containing 95% 50 mM ammonium bicarbonate, 5% acetonitrile and 0.1% formic acid and quanti ed by A205 nm protein and peptide methodology using a NanoDrop 2000c spectrophotometer (Thermo Fisher Scienti c).…”

Section: Protein Extraction and Digestionmentioning

confidence: 99%

Benzyladenine Effects On Polyamine Contents And Proteomic Profiles During In Vitro Shoot Development And On Ex Vitro Rooting In Dalbergia Nigra (Vell.) Allemão Ex Benth. (Fabaceae)

Pessanha

Aragão

Oliveira

et al. 2022

Preprint

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Dalbergia nigra is an endangered species from the Brazilian Atlantic Rainforest, and in vitro propagation can be applied for the conservation of this species. The aim of this study was to establish in vitro propagation and ex vitro rooting in D. nigra and evaluate the alterations in polyamines (PAs) and protein profiles during shoot development. The effect of MS and WPM culture media on in vitro germination was tested (%). For shoot induction, explants of apical and cotyledonary nodal segments from 45-day-old seedlings were inoculated in WPM culture medium supplemented with benzyladenine (BA; 0, 2.5 and 5 μM). Shoots obtained in vitro without and with 2.5 μM BA were rooted ex vitro with different concentrations (0, 100 and 500 μM) of indole-3-butyric acid (IBA). The best growth of seedlings was obtained in WPM culture medium. Treatment with 2.5 μM BA significantly increased the length of shoots by increasing free putrescine contents and the accumulation of proteins associated with shoot elongation, such as aspartate aminotransferase, elongation factor, calreticulin-3, and cell division cycle protein 48. Ex vitro rooting was obtained in all treatments of IBA, and the use of auxin was not necessary. The BA used for shoot multiplication significantly affected rooting, reducing the induction and number of roots but increasing the length of roots. This study showed the relevance of cytokinin, PAs and proteomic profiles on in vitro shoot development, as well as the auxin and cytokinin balance on ex vitro rooting in D. nigra.

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“…After protein precipitation, samples were resuspended in 7 M urea/2 M thiourea solution for proper resuspension. Protein digestion of aliquots of 100 µg protein from each biological replicate was performed using the lter-aided sample preparation (FASP) method as described by Wisniewski et al (2009) with modi cations performed by Reis et al (2021). The resulting peptides were quanti ed by the A 205 nm protein and peptide methodology using a NanoDrop 2000c spectrophotometer (Thermo Fisher Scienti c, Waltham, USA).…”

Section: Proteomic Analysismentioning

confidence: 99%

PEG induces maturation of somatic embryos of Passiflora edulis Sims ‘UENF Rio Dourado’ by differential accumulation of proteins and modulation of endogenous contents of free polyamines

Cruz

Almeida

Vale

et al. 2022

Plant Cell Tiss Organ Cult

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Sour passion fruit (Passi ora edulis Sims) has economic and social relevance and is an alternative crop mainly for family farming agriculture. The aim of this work was to evaluate the in uence of polyethylene glycol (PEG) on the maturation of somatic embryos associated with differential accumulation of proteins and changes in the endogenous polyamine (PA) content during somatic embryogenesis of P. edulis 'UENF Rio Dourado'. Maturation of somatic embryos was performed using embryogenic callus in MS culture medium with PEG 6% or without PEG (control). PEG 6% promoted the maturation of a signi cantly higher number of somatic embryos at globular and cotyledonary stages when compared to the control treatment. The higher somatic embryo formation induced by PEG 6% was associated with an increase in endogenous contents of free spermine, a PA with an important role in the maturation process of somatic embryogenesis cultures. PEG also promoted a signi cantly higher content of putrescine and total free PAs at the end of maturation, which was relevant for the increase in the number of somatic embryos.Comparative proteomic analyses of PEG 6%/control revealed that PEG 6% treatment induced the upaccumulation of proteins related to the ATP metabolic, glycolytic, generation of precursor metabolite energy, and the response to light stimulus processes. The down-accumulated proteins were related mainly to the cellular metabolic process. The use of PEG induced the maturation and development of somatic embryos of P. edulis Sims 'UENF Rio Dourado' by the differential accumulation of proteins and modulation of endogenous contents of PAs.

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Pretreatment free of 2,4-dichlorophenoxyacetic acid improves the differentiation of sugarcane somatic embryos by affecting the hormonal balance and the accumulation of reserves

Cited by 36 publications

References 61 publications

Creation of Borer Pests Resistance Genetically Engineering Peach (Prunus Persica L.) Plants by Cloning cry1Ab Gene

Creation of Borer Pests Resistance Genetically Engineering Peach (Prunus Persica L.) Plants by Cloning cry1Ab Gene

Benzyladenine Effects On Polyamine Contents And Proteomic Profiles During In Vitro Shoot Development And On Ex Vitro Rooting In Dalbergia Nigra (Vell.) Allemão Ex Benth. (Fabaceae)

PEG induces maturation of somatic embryos of Passiflora edulis Sims ‘UENF Rio Dourado’ by differential accumulation of proteins and modulation of endogenous contents of free polyamines

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