Prevailing acid determines the efficiency of oleaginous fermentation from volatile fatty acids

Morales-Palomo, S.; González‐Fernández, Cristina; Tomás‐Pejó, Elia

doi:10.1016/j.jece.2022.107354

Cited by 18 publications

(27 citation statements)

References 36 publications

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“…Lipid contents of 43.4% w/w [ 30 ] and > 70% w/w (unpublished results) were achieved with the same Y. lipolytica ACA-DC 50109 strain and determined with the methodology described herein. These facts indicated that the ф fl could also be used to quantify large amounts of lipids not being affected by possible saturations.…”

Section: Resultsmentioning

confidence: 99%

Key role of fluorescence quantum yield in Nile Red staining method for determining intracellular lipids in yeast strains

Morales-Palomo

Liras

González‐Fernández

et al. 2022

Biotechnol Biofuels

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Background Microbial lipids are found to be an interesting green alternative to expand available oil sources for the chemical industry. Yeasts are considered a promising platform for sustainable lipid production. Remarkably, some oleaginous yeasts have even shown the ability to grow and accumulate lipids using unusual carbon sources derived from organic wastes, such as volatile fatty acids. Recent research efforts have been focused on developing rapid and accurate fluorometric methods for the quantification of intracellular yeast lipids. Nevertheless, the current methods are often tedious and/or exhibit low reproducibility. Results This work evaluated the reliability of different fluorescence measurements (fluorescence intensity, total area and fluorescence quantum yield) using Nile Red as lipid dye in two yeast strains (Yarrowia lipolytica ACA-DC 50109 and Cutaneotrichosporon curvatum NRRL-Y-1511). Different standard curves were obtained for each yeast specie. Fermentation tests were carried with 6-month difference to evaluate the effect of the fluorometer lamp lifetime on lipid quantification. Conclusions Fluorescence quantum yield presented the most consistent measurements along time and the closer estimations when compared with lipids obtained by conventional methods (extraction and gravimetrical determination). The need of using fluorescence quantum yield to estimate intracellular lipids, which is not the common trend in studies focused on microbial lipid production, was stressed. The information here provided will surely enable more accurate results comparison.

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Section: Resultsmentioning

confidence: 99%

Key role of fluorescence quantum yield in Nile Red staining method for determining intracellular lipids in yeast strains

Morales-Palomo

Liras

González‐Fernández

et al. 2022

Biotechnol Biofuels

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“…In order to mimic the same C:N ratio presented in RD 1, the C:N ratio in SM was set at 12 (1.3 g N‐NH 4 + /L). Under such low C:N ratio, the expected lipid accumulation would be low, considering the importance of high C:N ratios when using both sugar‐based and SCFAs‐rich media for oleaginous yeast fermentation (Amza et al, 2019 ; Morales‐Palomo, González‐Fernández, & Tomás‐Pejó, 2022 ; Wei et al, 2019 ).…”

Section: Resultsmentioning

confidence: 99%

“…The oleaginous yeast used in this study due to its ability to accumulate more than 20% w/w DW and consume SCFAs (Dourou et al, 2017 ) was Y. lipolytica ACA DC 50109 (from the Agricultural University of Athens' culture collection). The yeast was preserved under the conditions described in Morales‐Palomo, González‐Fernández, & Tomás‐Pejó, 2022 . One colony of the strain was inoculated in YPD liquid medium (20 g/L peptone, 20 g/L glucose and 10 g/L yeast extract), with an initial pH of 7.0 (no adjustment was needed), in order to prepare a preinocula which was incubated overnight at 150 rpm and 27 °C in a rotary shaker until the late exponential growth phase was reached.…”

Section: Methodsmentioning

confidence: 99%

“…SCFAs were quantified by liquid chromatography as described in Morales‐Palomo, González‐Fernández, & Tomás‐Pejó, 2022 . In total, 5 mM H 2 SO 4 was used as mobile phase at a flow rate of 0.6 ml/min and was used in isocratic mode for the elution.…”

Section: Methodsmentioning

confidence: 99%

“…SCFAs are organic acids that can be obtained through anaerobic fermentation of organic wastes. Recent investigations have indeed shown that SCFAs are promising feedstocks for lipid obtention (Llamas, Tomás‐Pejó, & González‐Fernández, 2020 ; Morales‐Palomo, González‐Fernández, & Tomás‐Pejó, 2022 ) and could be, thereby, a solution against the high cost of sugar substrates while offering a novel route for organic waste valorization.…”

Section: Introductionmentioning

confidence: 99%

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Phosphate limitation as crucial factor to enhance yeast lipid production from short‐chain fatty acids

Morales-Palomo

Tomás‐Pejó

González‐Fernández

2022

Microbial Biotechnology

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Microbial lipids for chemical synthesis are commonly obtained from sugar‐based substrates which in most cases is not economically viable. As a low‐cost carbon source, short‐chain fatty acids (SCFAs) that can be obtained from food wastes offer an interesting alternative for achieving an affordable lipid production process. In this study, SCFAs were employed to accumulate lipids using Yarrowia lipolytica ACA DC 50109. For this purpose, different amounts of SCFAs, sulfate, phosphate and carbon: phosphate ratios were used in both synthetic and real SCFAs‐rich media. Although sulfate limitation did not increase lipid accumulation, phosphate limitation was proved to be an optimal strategy for increasing lipid content and lipid yields in both synthetic and real media, reaching a lipid productivity up to 8.95 g/L h. Remarkably, the highest lipid yield (0.30 g/g) was achieved under phosphate absence condition (0 g/L). This fact demonstrated the suitability of using low‐phosphate concentrations to boost lipid production from SCFAs.

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Microbial Production of Oleochemicals

Greses

Llamas

Morales-Palomo

et al. 2022

Handbook of Biorefinery Research and Technology

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Prevailing acid determines the efficiency of oleaginous fermentation from volatile fatty acids

Cited by 18 publications

References 36 publications

Key role of fluorescence quantum yield in Nile Red staining method for determining intracellular lipids in yeast strains

Key role of fluorescence quantum yield in Nile Red staining method for determining intracellular lipids in yeast strains

Phosphate limitation as crucial factor to enhance yeast lipid production from short‐chain fatty acids

Microbial Production of Oleochemicals

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