Prevalence and antibiotic susceptibility pattern of ESBL producing Klebsiellae isolated from clinical specimens in a Nigerian tertiary hospital

Fadeyi, A; Zumuk, CP; Raheem, RA; Nwabuisi, C; Desalu, Olufemi Olumuyiwa

doi:10.4314/ajid.v10i1.7

Cited by 9 publications

(9 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The ESBL-producing isolates observed in this study were E. coli and Klebsiella sp. ; this is in agreement with other studies that reported that both isolates contain ESBL enzyme [13,18]. ESBL has been reported to confer resistance to all β-lactam antibiotics except cephamycin and carbapenems [29].…”

Section: Discussionsupporting

confidence: 93%

“…ESBL-producing bacterial isolates have been found in both hospital and community settings [6,16]. Furthermore, ESBL-producing enterobacteriaceae may acquire resistance to non-β-lactam antibiotics in the community [17][18]. The present study determines the occurrence of ESBL among Gram-negative bacilli (GNB) isolated from the urine of apparently heathy individuals at the Obafemi Awolowo University.…”

Section: Introductionmentioning

confidence: 95%

See 1 more Smart Citation

Extended-spectrum β-lactamase Occurrence among Gram-negative Bacilli Isolated from Urine of Apparently Healthy Individuals at Obafemi Awolowo University, Ile-Ife, Nigeria

Torimiro¹

2018

JAMB

View full text Add to dashboard Cite

Methodology: Mid-stream urine samples were collected from 70 apparently healthy and consenting participants. The samples were inoculated onto MacConkey agar and incubated at 37°C; the isolates were identified using standard methods. The antibiotic susceptibility profile and ESBL test were performed using standard methods. Results: A total of 47 (67.1%) monomicrobial GNB were isolated. Escherichia coli (15, 31.9%) was the most predominant isolate identified. All isolates were multi-drug resistant; β-lactamase was produced in 17 (36.2%) isolates and ESBL, in only two isolates. ESBL-producing isolates showed resistance to non-β-lactam antibiotics.

show abstract

Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 95%

Extended-spectrum β-lactamase Occurrence among Gram-negative Bacilli Isolated from Urine of Apparently Healthy Individuals at Obafemi Awolowo University, Ile-Ife, Nigeria

Torimiro¹

2018

JAMB

View full text Add to dashboard Cite

show abstract

“…also had considerable resistance to other antibiotics including ofloxacin (94.4%), nitrofurantoin (72.2%) and 88.9% resistance to nalidixic acid, gentamicin and levofloxacin. This is similar to the findings of Fadeyi et al [6] and Humayun et al [21] that reported the resistance of Klebsiella spp. to fluoroquinolone, aminoglycoside and sulfamethoxazole in Nigeria and Pakistan respectively.…”

Section: Discussionsupporting

confidence: 92%

“…Strains were resistant to fluoroquinolones, carbapenem, third and fourth generation cephalosporins. In a similar study, Fadeyi et al [6] in Nigeria reported Klebsiella spp. of clinical origin that displayed high resistance to ceftazidime (100%), cefotaxime (94.0%), ciprofloxacin (70.0%) and trimethoprim-sulphamethoxazole (92.0%).…”

Section: Introductionmentioning

confidence: 67%

Efflux pump activity, biofilm formation and antibiotic resistance profile of Klebsiella spp. isolated from clinical samples at Lagos University Teaching Hospital

et al. 2020

View full text Add to dashboard Cite

Objective: Nosocomial and community acquired multidrug resistant Klebsiella infections are wide spread resulting in high morbidity and mortality due to limited number of antibiotics treatment options. This study investigated efflux pump activity, biofilm forming potential and antibiotic susceptibility profile of Klebsiella spp. isolated from clinical samples in a tertiary hospital in Lagos Nigeria. Eighteen clinical Klebsiella spp. isolated from urine, blood and sputum were subjected to antibiotic susceptibility testing using the disc diffusion method. Efflux pump activity was evaluated by the ethidium bromide cartwheel method and biofilm forming ability was determined by the tissue culture plate technique. Results: All 18 (100%) Klebsiella isolates were resistant to cefuroxime, cefixime, amoxicillin − clavulanate, ampicillin + cloxacillin, cefotaxime, and imipenem. Seventeen (94.4%) were resistant to ofloxacin while sixteen (88.9%) were resistance to nalidixic acid, Gentamicin and levofloxacin. All Klebsiella isolates possessed active efflux pump with the ability to form biofilm. However, their biofilm forming capabilities varied as 4 (22.2%) were strong, 3 (16.7%) were moderate and 11 (61.1%) were weak biofilm formers. Findings in this study reveal multiple factors at play in mediating the high level of antibiotic resistance observed in Klebsiella isolates. Hence a multifaceted approach is advocated in managing the infections caused by the pathogen.

show abstract

“…The udy showed that 39 -90% of seven common bacterial isolates were resistant to Amoxicillin + Clavulanic acid, similar high level of microbial resistance was also observed for Cotrimoxazole [29 -84%], Tetracycline [17 77%], Ceftriaxone [6 -50%] and Genta -40%]. The high level of antibiotic resistance level in this study is comparable to many other studies, though differences exist in the magnitude [58][59][60][61][62].…”

Section: Fig 2 Age Distributionsupporting

confidence: 83%

Empirical Antibiotic Prescription Pattern among Patients in a Nigerian Tertiary Hospital, is There Evidence of Irrationality?

Paul

Abdulmalik

2019

JAMMR

View full text Add to dashboard Cite

Background: Resistance to antibiotics is spreading rapidly around the world with its associated morbidity and mortality. Infections are becoming increasingly difficult to treat resulting in increased cost of medical care. In low income countries with high infectious disease burden, antibiotic resistance is reported to be accelerated by irrational prescriptions in health facilities. In the absence of adequate resources, many clinicians engage in empirical antibiotic prescriptions some of which their appropriateness is questionable. There is need for laboratory evidence to justify empirical antibiotic use in the light of increasing resistance to commonly prescribed antibiotics. Aims: This study aims to determine empirical antibiotic prescription pattern and to determine rationality using resistance profile of common bacterial isolates in hospitals. Methods: Antibiotic prescriptions in the NHIS department and antibiogram records were obtained from pharmacy and laboratory records respectively. Analysis was carried out using descriptive statistics and comparison between antibiotics prescribed and their respective resistance pattern were compared to determine rationality. Results and Discussion: The Penicillins and Quinolones were the most prescribed class of antibiotics and resistance range between 30 – 90% and 3 – 23% respectively. Resistance to other antibiotics was high thus making empirical prescriptions irrational in most of the cases. These findings have been consistently reported in several studies so widespread empirical antibiotic prescriptions are not in tandem with principles of rational drug use. Conclusion: Antibiotic resistance is common among hospital isolates, so there is need to emphasize that prescriptions be based on laboratory evidence of microbial sensitivity.

show abstract

Prevalence and antibiotic susceptibility pattern of ESBL producing Klebsiellae isolated from clinical specimens in a Nigerian tertiary hospital

Cited by 9 publications

References 6 publications

Extended-spectrum β-lactamase Occurrence among Gram-negative Bacilli Isolated from Urine of Apparently Healthy Individuals at Obafemi Awolowo University, Ile-Ife, Nigeria

Extended-spectrum β-lactamase Occurrence among Gram-negative Bacilli Isolated from Urine of Apparently Healthy Individuals at Obafemi Awolowo University, Ile-Ife, Nigeria

Efflux pump activity, biofilm formation and antibiotic resistance profile of Klebsiella spp. isolated from clinical samples at Lagos University Teaching Hospital

Empirical Antibiotic Prescription Pattern among Patients in a Nigerian Tertiary Hospital, is There Evidence of Irrationality?

Contact Info

Product

Resources

About