Prevalence and Molecular Characterization of AmpC-Producing Clinical Isolates of<i>Escherichia coli</i>from Southeastern Iran

Shayan, Sara; Bokaeian, Mohammad; Shahraki, Shahram

doi:10.1089/mdr.2013.0087

Cited by 7 publications

(6 citation statements)

References 12 publications

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“…In Asia, the percentage of clinical E.coli isolates that is pAmpC positive varies over a wider range. The lowest prevalence has been reported in studies in Japan (0.12% and 1.7% of E.coli [46, 47]), Iran (2.8% of E.coli [48]) and China (2% of E.coli [49, 50]). In a study in Turkey, 10.9% of clinical E.coli isolates carried pAmpC genes [23].…”

Section: Discussionmentioning

confidence: 99%

Prevalence of plasmid-mediated AmpC in Enterobacteriaceae isolated from humans and from retail meat in Zagazig, Egypt

Rensing

Abdallah

Koek

et al. 2019

Antimicrob Resist Infect Control

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Background The objective of this study was to determine the prevalence of plasmid-mediated AmpC (pAmpC) among Enterobacteriaceae isolated from humans and from retail meat in Egypt. Methods Enterobacteriaceae were isolated from patients with suspected bloodstream infection, human fecal samples, retail chicken meat samples and retail sheep meat samples. All group I Enterobacteriaceae were analyzed for presence of pAmpC genes by PCR. Antibiotic susceptibility testing was performed in all pAmpC positive isolates, followed by phenotypic and genotypic ESBL and carbapenemase testing on indication. Results The prevalence of pAmpC among group I Enterobacteriaceae isolated from 225 patients with bloodstream infection was 5.6% [95%CI 2.2–13.4]. Among 100 patients with community-onset gastroenteritis the prevalence in fecal samples was 4.8% [95%CI 2.1–10.7]. The prevalence among 112 chicken carcasses and 100 sheep meat samples was 2.4% [95%CI 0.7–8.4] and 1.1% [95%CI 0.2–5.7], respectively. In half of the AmpC positive isolates we detected an ESBL gene and 2 isolates harbored a carbapenemase gene. In five isolates there was resistance to at least three important alternative antibiotic drugs. Conclusions We consider the prevalence of pAmpC in Egypt, as found in our study, moderately low. To follow future trends in prevalence of pAmpC worldwide, a standardized screening algorithm for the detection of pAmpC is needed.

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Section: Discussionmentioning

confidence: 99%

Prevalence of plasmid-mediated AmpC in Enterobacteriaceae isolated from humans and from retail meat in Zagazig, Egypt

Rensing

Abdallah

Koek

et al. 2019

Antimicrob Resist Infect Control

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“…This study showed that prevalence of bla CMY-2 , bla CMY-42 , and bla DHA genes among AmpC β-lactamases producers were 39%, 1%, and 17.5%, respectively. In a study carried out by Shayan et al [23] in Iran, the prevalence of bla CMY gene among 392 isolates of E. coli was investigated. They reported that 13 (3.3%) isolates were identified as AmpC producers, which 11 of the 13 isolates contained the bla CMY gene.…”

Section: Discussionmentioning

confidence: 99%

Emergence of fosfomycin resistance among isolates of Escherichia coli harboring extended-spectrum and AmpC β-lactamases

Bahramian

Eslami

Hashemi

et al. 2017

AMicr

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Urinary tract infection (UTI) is a common type of infectious disease globally. The aim of this study was to detect the frequency of fosA3 and fosC2 genes in extended-spectrum β-lactamases (ESBL) and bla, bla, and bla genes in AmpC β-lactamases-producing isolates of Escherichia coli. In total, 120 isolates of E. coli were collected from three teaching hospitals between March 2014 and February 2015. Antibiotic susceptibility tests were carried out by disk diffusion method. The presence of bla, bla, bla, fosA3, and fosC2 genes was detected by polymerase chain reaction (PCR) and sequencing. Of the 120 strains, 92 (76.6%) were identified as ESBL producers, 30 (25%) were determined as AmpC β-lactamase producers, and 24 (20%) had both ESBL and AmpC β-lactamase enzymes. Imipenem, fosfomycin, and nitrofurantoin had the best effect against isolates of E. coli. PCR assay demonstrated that the frequency of bla, bla, and bla genes among AmpC β-lactamases-producing strains were 39%, 1%, and 17.5%, respectively. This study reports the first detection of fosfomycin resistance in Iran. This study indicated the increasing prevalence of UTI isolates of E. coli-harboring ESBL and AmpC β-lactamases genes in Iran. Therefore, due to the high rate of bla and bla genes and emergence of fosfomycin-resistant E. coli isolates, we recommend continuous monitoring of antibiotic resistance as well as attention to guidelines of infection controls.

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“…[ 19 20 ] In another Iranian study, 3.3% of E. coli isolates produced AmpC β-lactamases. [ 13 ] A study from Canada showed that the annual incidence rates of AmpC were 1.7, 4.3, 11.2, and 15 per 100,000 residents for each year, respectively. [ 21 ] Jabeen et al .…”

Section: Discussionmentioning

confidence: 99%

“…This resistance mechanism has been found around the world, can cause nosocomial outbreaks, and appears to be increasing in prevalence. [ 13 ] This study was undertaken to characterize ESBL and AmpC producers among E. coli by PCR, which were initially screened by phenotypic method.…”

Section: Introductionmentioning

confidence: 99%

Detection of ESBL- and AmpC-producing E. coli isolates from urinary tract infections

Shayan

Bokaeian

2015

Adv Biomed Res

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Background:Extended-spectrum β-lactamases (ESBLs) and AmpC enzymes have been observed in virtually all species of the family Enterobacteriaceae. The β-lactamase producing bacteria cause many serious infections, including urinary tract infections. These enzymes are predominantly plasmid mediated. There are no recommended guidelines for detection of this resistance mechanism and there is a need to address this issue as much as the detection of ESBLs. This study was undertaken to characterize ESBL and AmpC producers among Escherichia coli by polymerase chain reaction (PCR), which were initially screened by phenotypic method.Materials and Methods:A total of 90 isolates of E. coli were recovered from the urinary tract during a 7-month period, and were screened for ESBLs and AmpC production by disk diffusion test using cefoxitin (30 μg) disks and confirmed by combined disk diffusion test using phenyl boronic acid. The presence of genes encoding CIT, FOX, and TEM was detected by PCR.Results:On disk diffusion test, 59 of 90 isolates were resistant to third generation of cephalosporins; of these 37 (62.7%) and 3 (5%) were ESBL and AmpC producers, respectively. PCR showed that 29 (49.1%) and 3 (5%) were positive for blaTEM and blaCMY-2, respectively.Conclusion:ESBL- and AmpC-producing E. coli isolates cause significant resistance to cephalosporin. There is a need for a correct and reliable phenotypic test to identify AmpC β-lactamases and to discriminate between AmpC and ESBL producers. This work showed that boronic acid can differentiate ESBL enzymes from AmpC enzymes.

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Prevalence and Molecular Characterization of AmpC-Producing Clinical Isolates ofEscherichia colifrom Southeastern Iran

Abstract: AmpC hyperproducing E. coli isolates exhibit significant resistance to cephalosporins. This work showed that strains hyperproducing chromosomal AmpC could be as frequent as strains with plasmid-mediated AmpC hyperproduction.

Cited by 7 publications

References 12 publications

Prevalence of plasmid-mediated AmpC in Enterobacteriaceae isolated from humans and from retail meat in Zagazig, Egypt

Prevalence of plasmid-mediated AmpC in Enterobacteriaceae isolated from humans and from retail meat in Zagazig, Egypt

Emergence of fosfomycin resistance among isolates of Escherichia coli harboring extended-spectrum and AmpC β-lactamases

Detection of ESBL- and AmpC-producing E. coli isolates from urinary tract infections

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