Prevalence of dengue virus serotypes in the 2017 outbreak in Peshawar, KP, Pakistan

Khan, Najeeb Ullah; Danish, Lubna; Khan, Hydayat Ullah; Shah, Maryam; Ismail, Muhammad; Ali, Ijaz; Petruzziello, Arnolfo; Sabatino, Rocco; Guzzo, Annunziata; Botti, Gerardo; Iqbal, Aqib

doi:10.1002/jcla.23371

Cited by 18 publications

(11 citation statements)

References 29 publications

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“…In our study, serotypes 2 and 3 were the predominant DENV serotypes between 2012 and 2020. Our study agreed with the Khan et al studies performed in the Pakistani population ( Khan et al, 2020 ).…”

Section: Discussionsupporting

confidence: 93%

Epidemiological screening and serotyping analysis of dengue fever in the Southwestern region of Saudi Arabia

Alshabi

Marwan

Fatima

et al. 2022

Saudi Journal of Biological Sciences

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Dengue is an acute systemic viral disease that has been developed globally in both chronic and epidemic transmission periods. Dengue virus (DENV) is a member of the Flavivirus genus of the Flaviviridae family, which endangers public health. Limited studies have been performed in the Saudi Arabia and there are no epidemiological as well as molecular screening of DENV in the Southwestern region and this current study was conducted on the epidemiology of dengue in the Southwestern region of Saudi Arabia. Simultaneously, we have screened the 100 patients for DENV using the real-time reverse transcriptase polymerase chain reaction (RT-PCR) assay. The current study results confirmed that 6637 people from various hospitals and locations in Jazan, Southwestern regions, were enrolled in this study from 2012 to 2020. The overall mean age was 30.02 ± 18.01 years, with 62.8% of males and 37.2% of females enrolled. This study included nearly three-fourths of the Saudi participants and one-fourth of the expatriates, and 56.6% of the positive cases were enrolled. In 2019, the most instances were enrolled, with 44% of positive cases. When screened using the RT-PCR assay, 93% of the positive patients were recruited, according to the quality control analysis. In conclusion, the current study results confirmed the prevalence of DENV was increased drastically since 2012 to 2020. High number of cases were registered prior to the Pandemic. The screening for DENV was performed with RT-PCR assay and NSI antigen should also be implemented to cross-check the results which was previously performed with RT-PCR analysis.

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Section: Discussionsupporting

confidence: 93%

Epidemiological screening and serotyping analysis of dengue fever in the Southwestern region of Saudi Arabia

Alshabi

Marwan

Fatima

et al. 2022

Saudi Journal of Biological Sciences

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“…According to our findings, the predominant serotypes were DENV-2 (45.8%) and DENV-3 (50.4%), with DENV-1 (1.9%) and DENV-4 (0.4%) the least common serotypes (Figure 4), suggesting this outbreak is a continuation of the previous outbreak (2017). Our hypothesis is confirmed by recent studies conducted in the area (Peshawar) (66,67). The epidemiological dynamics of dengue disease in a region are influenced by multiple serotypes (64).…”

Section: Clinical Symptoms and Disease Severity Of Dengue Feversupporting

confidence: 88%

A cross-sectional study to assess the epidemiological situation and associated risk factors of dengue fever; knowledge, attitudes, and practices about dengue prevention in Khyber Pakhtunkhwa Province, Pakistan

Khan

Adil²,

Wang

et al. 2022

Front. Public Health

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BackgroundDengue fever has been responsible for around 12 countrywide large outbreaks in Pakistan, resulting in 286,262 morbidities and 1,108 deaths. Khyber Pakhtunkhwa (KP) is the most recently impacted province. This study aimed to investigate the molecular, epidemiological, and potential elements that contribute to increasing dengue transmission patterns, and knowledge, attitude, and practice (KAP) toward dengue in KP province.MethodThis cross-sectional community-based study was conducted (June-December, 2021) in two phases. Phase I involved the epidemiological (n = 5,242) and molecular analysis of DENV in 500 randomly collected blood samples of the 2021 dengue outbreak in KP. Phase II focused on assessing dengue-KAP levels in healthy communities (n = 14,745, aged >18 years), adopting a cross-sectional clustered multistage sampling in eight districts (dengue-hotspot vs. non-hotspot) of KP. Chi-square tests and logistic regression analysis were applied.ResultsPeshawar district had the highest dengue cases (60.0%) associated with the predominant co-circulation of DENV-2 (45.8%) and DENV-3 (50.4%) serotypes. A rise in cases was reported in October (41.8%) followed by September (27.9%) and August (14.4%; p < 0.001). Males (63.7%, p < 0.001) and individuals aged 16–30 years (37.0%, p < 0.001) were highly affected. General workers (18.0%), families with a monthly income of 10,000–20,000 Pak rupees (50.5%), unmarried (71.0%), uneducated (31%), families with higher human density (>10 individuals per household), and those (29.0%) who faced power outages for more than 7/24 h were the most affected. Moreover, co-morbidities like renal failure and bronchial asthma were associated with disease severity. A community survey on KAP revealed that an average of 74, 60, and 43% of the participants demonstrated good knowledge, attitudes, and dengue preventive practices, respectively.ConclusionMultiple poor socioeconomic elements are influencing dengue fever transmission in the province. Higher KAP levels may explain the low frequency of dengue in non-hotspot districts. Our study emphasizes the need for effective and long-term public health education, strengthened vector surveillance, and expanded laboratory capacity for better diagnosis and management of dengue cases to better predict the burden and seasonality of disease in the country.

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“…By the end of 2017, a severe dengue outbreak with 100% DENV-2 along with 5% samples having coinfection with DENV-3 was reported in a study[26]. There are several reports on DENV-4 circulating in Pakistan [27, 28], although the present small-scale study does not detect any case in 2022. There have been several reported shifts in dengue virus serotypes, genotypes, or lineages that have resulted in increased severity of dengue infection[11, 25, 26].…”

Section: Discussionmentioning

confidence: 53%

Genomic Characterization of Dengue Virus Outbreak in 2022 from Pakistan

Umair

Rehman

Haider

et al. 2022

Preprint

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Pakistan, a dengue endemic country has encountered several outbreaks during the past decade. The current study aimed to explore the serotype and genomic diversity of dengue virus responsible for the 2022 outbreak in Pakistan. During August-October 2022, blood samples (n=436) were collected from dengue patients, among which 64.4% (n=281) were positive based on RT-PCR. A subset of DENV-2 and DENV-1 samples were further subjected to whole-genome sequencing. In terms of gender and age, dengue infection was more prevalent in male patients (62.9%) with more adults (77.5%) being infected. Moreover, serotyping revealed DENV-2 to be most predominant serotype (64%; n=180), followed by DENV-1 (35.2%; n=99) and DENV-3 (0.35%; n=1). Phylogenetic analysis of sequenced samples indicates that all the samples (n=8) belong to the DENV-2 Cosmopolitan genotype, falling within a single monophyletic clade that is closely related to sequences from China and Singapore in 2018. Dengue virus dynamics reported in the current study warrants large scale genomic surveillance in order to better respond to future outbreaks.

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Prevalence of dengue virus serotypes in the 2017 outbreak in Peshawar, KP, Pakistan

Cited by 18 publications

References 29 publications

Epidemiological screening and serotyping analysis of dengue fever in the Southwestern region of Saudi Arabia

Epidemiological screening and serotyping analysis of dengue fever in the Southwestern region of Saudi Arabia

A cross-sectional study to assess the epidemiological situation and associated risk factors of dengue fever; knowledge, attitudes, and practices about dengue prevention in Khyber Pakhtunkhwa Province, Pakistan

Genomic Characterization of Dengue Virus Outbreak in 2022 from Pakistan

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