Back ground: Low back pain (LBP) is one of the most common work-related health problems among hospital workers. Work-related activities such as twisting, bending, sustained posture, repeated movements are regarded as associated risk factors for low back pain. The aim of this study is to assess the prevalence and associated risk factors of Low Back Pain among hospital workers. Methodology: An institution based cross sectional survey design was conducted among employees in Adama Hospital Medical College (AHMC), Adama Ethiopia. Descriptive statistics, bivariate, and multiple regression analyses were performed. p<0.1 in univariate is considered for multivariate analysis and p<0.05 was used to define statistical significance. Result: Of 263, 137(52.1%) were males and 126(47.9%) were females. The result indicated that the mean and median age of the respondents were 33.5 and 33 years respectively. The life time prevalence of LBP was found to be 50.6% and the 12 month LBP prevalence was 41.4%. Staff not participating in any physical activity (exercise) were more likely to report presence of low back pain (AOR (Adjusted Odd Ratio)=1.75, CI, 1.02-3.02). On the other hand, staff who worked sitting more than 6 hours were associated with increased risk of low back pain(AOR=2.78, CI, 1.09-7.07). Perceived stress often increased risk of low back pain (AOR=2.60, CI, 1.06-6.36). Conclusion: LBP was significantly associated with the obesity, stressed often, those worked in seated position more than six hours and those with long year experience; but doing physical exercise has rather a preventive effect. The findings of this study suggest that primary prevention of LBP should be considered.