Prevalence of Treponema Species Detected in Endodontic Infections: Systematic Review and Meta-regression Analysis

Leite, Fábio Renato Manzolli; Nascimento, Gustavo G.; Demarco, Flávio Fernando; Gomes, Brenda Paula Figueiredo de Almeida; Pucci, César Rogério; Martinho, Frederico Canato

doi:10.1016/j.joen.2015.01.020

Cited by 13 publications

(4 citation statements)

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“…Obligate anaerobes and Gram-negative bacterial species play an important role in the development of symptoms of primary apical periodontitis (Jacinto et al, 2003;Martinho et al, 2014;Vickerman et al, 2007). Several key species may play an important role in the symptomatic state (Leite et al, 2015;Nobrega et al, 2016aNobrega et al, , 2016bVengerfeldt et al, 2014;Zargar et al, 2020), but other studies found no association between specific bacteria and clinical symptoms (Bronzato et al, 2021). Post-treatment apical periodontitis may be asociated with a more diverse microbiota than primary apical periodontitis (Machado et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Potential relationship between clinical symptoms and the root canal microbiomes of root filled teeth based on the next‐generation sequencing

et al. 2021

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Aim: To determine the microorganism in root canal systems of root filled teeth with periapical disease and their relationship with clinical symptoms using nextgeneration sequencing. Methodology:The roots of 10 extracted teeth were collected from 10 patients who presented with post-treatment apical periodontitis (PTAP; six with symptoms and four without symptoms). Each root was divided horizontally into two parts (apical and coronal segments) and cryo-pulverized. Microbial communities were detected using 16S rDNA hypervariable V3-V4 region. The diversity, principal coordinate analysis and linear discriminant analysis effect size were performed in the symptomatic and asymptomatic groups (apical and coronal parts respectively). A Mann-Whitney test and an analysis of similarities were applied for intergroup analysis, at a significance level of 5%.Results: A total of 23 phyla, 257 genera and 425 species were detected. Firmicutes was the most abundant phylum in all samples. Three phyla (Fusobacteria, Synergistetes and unidentified_Bacteria) and seven genera (Fusobacterium, Porphyromonas, Phocaeicola, Olsenella, Campylobacter, Tannerella and Fretibacterium) were significantly more abundant in the symptomatic patients (p < .05), whereas asymptomatic patients had more Sphingomonas. The species more significantly abundant in the symptomatic samples were Porphyromonas gingivalis, Phocaeicola abscessus, Campylobacter showae, Tannerella forsythia and Olsenella uli (p < .05).Conclusions: A greater microbial diversity was observed in root filled teeth with PTAP compared to earlier reports. Several genera and species in root canal systems might be associated with clinical symptoms of PTAP.

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Section: Introductionmentioning

confidence: 99%

Potential relationship between clinical symptoms and the root canal microbiomes of root filled teeth based on the next‐generation sequencing

et al. 2021

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“…40 A meta-analysis showed the heterogeneity in the prevalence rates of the Treponema species in primary and secondary infections, whether symptomatic or not. 41 The authors of a previous study emphasized the importance of these species, particularly in cases of acute primary infection. The participation of T. denticola in the pathogenesis of acute apical abscess was evaluated using real-time PCR.…”

Section: Discussionmentioning

confidence: 99%

Qualitative and quantitative molecular analysis of bacteria in root canals of primary teeth with pulp necrosis

et al. 2020

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Information about bacterial diversity, such as the number of each species in the root canals of primary teeth, contributes to improving our effective management of infections of endodontic origin in primary teeth. This study made a qualitative and quantitative assessment of the bacteria in the root canals of primary teeth with necrotic pulp, using the fluorescence in situ hybridization (FISH) technique. Thirty-one primary teeth with pulp necrosis from 31 children were evaluated using the FISH technique, to detect the presence and density of Aggregatibacter actinomycetemcomitans, Campylobacter rectus, Enterococcus faecalis, Fusobacterium nucleatum, Porphyromonas gingivalis, Prevotella intermedia, Prevotella nigrescens, Streptococcus, Streptococcus mutans, Streptococcus sobrinus, Tannerella forsythia and Treponema denticola. Descriptive measures explained the data related to density, and Student's t-test assessed the differences among the densities of each bacterium, according to signs and symptoms. The bacterial density was paired and correlated. All bacteria tested were detected and identified in all the samples. The average number of bacterial individuals from each species ranged from 1.9 x 10 8 cells/mL (S. mutans) to 3.1 x 10 8 cells/mL (F. nucleatum) (p > 0.05). The sum of the mean counts of each bacterium represented almost 80% of the entire microbial community. Patients with pain had significantly more T. denticola, and those with edema showed a greater density of Streptococcus and P. nigrescens (p < 0.05). This study revealed that all 12 bacteria evaluated were found in all primary teeth with pulp necrosis. There was no predominance among the species studied; all species had a similar number of individuals.

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“…Bacteria that fit these characteristics include gram-negative anaerobes such as Fusobacterium spp., Treponema spp., Prevotella spp., Porphyromonas spp., and Tannerella forsythia as well as gram-positive facultative organisms such as Strepotococcus spp., Parvimonas spp., Propioninbacterium spp., Actinomyces spp., and Eubacterium spp. [34][35][36][37][38][39] . In addition, Enterococcus faecalis has not only been identified as a prevalent bacterial species in cases with persistent disease 40 ; it appears to promote the survival of other bacteria in the endodontic environment and their resistance to treatment 41,42 .…”

Section: Microflora Of Endodontic Infections In Immature Teethmentioning

confidence: 99%

Contemporary Microbial and Antimicrobial Considerations in Regenerative Endodontic Therapy

Fouad

2020

Journal of Endodontics

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Introduction: Regenerative endodontic therapy (RET) has gained considerable attention and wide approval. Although it is being performed routinely, the outcomes remain unpredictable, and the optimal approaches for treatment are not established. It has been shown that bacterial persistence in root canals in these cases significantly interferes with healing and root maturation. However, few objective clinical studies have evaluated the complex microflora present in the infections or the efficacy of various clinical procedures. In addition, the extent of the infection and biofilm maturation in immature teeth with necrotic pulp has been understudied. Furthermore, most models used in preclinical evaluation of these issues do not fully elucidate the complexity or variability of the clinical situation. Results and Conclusion: In this review, the main biological and clinical problems pertaining to RET will be discussed. Contemporary analysis of complex microbial communities will be reviewed with emphasis on how these types of analyses can provide clinically useful data. In addition, current and proposed approaches for the effective disinfection of the root canal environment without interference with stem cell viability or integrity of the dentin matrix in these cases will be explored. The future of research in this field, including better and more customizable approaches in RET, in light of recent technological advances and progress in endodontics, will be outlined.

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Prevalence of Treponema Species Detected in Endodontic Infections: Systematic Review and Meta-regression Analysis

Cited by 13 publications

References 77 publications

Potential relationship between clinical symptoms and the root canal microbiomes of root filled teeth based on the next‐generation sequencing

Potential relationship between clinical symptoms and the root canal microbiomes of root filled teeth based on the next‐generation sequencing

Qualitative and quantitative molecular analysis of bacteria in root canals of primary teeth with pulp necrosis

Contemporary Microbial and Antimicrobial Considerations in Regenerative Endodontic Therapy

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