Preventive effect of different toothpastes on enamel erosion: AFM and SEM studies

Lombardini, Marco; Ceci, Matteo; Colombo, Marco; Bianchi, Stefano; Poggio, Claudio

doi:10.1002/sca.21132

Cited by 34 publications

(38 citation statements)

References 23 publications

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“…They were excluded in the case of white spot lesions, caries, hypoplasia, cracks, and any previous treatment with chemical agents. Finally, disinfection was performed by dipping the teeth in a 5% sodium hypochlorite solution for one hour, which could not alter the enamel surface [32,33]. Each specimen was cut at the enamel-dentin junction using a high-speed diamond rotary bur (Komet Brasseler GmbH & Co., Lemgo, Germany) with a water-air spray.…”

Section: Specimen Preparationmentioning

confidence: 99%

In Vitro Re-Hardening of Bleached Enamel Using Mineralizing Pastes: Toward Preventing Bacterial Colonization

et al. 2020

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The search for materials able to remineralize human hard tissues is a modern medical challenge. In this study, the protective effect on the enamel microhardness by a paste based on hydroxyapatite and sodium fluoride (Remin Pro) was evaluated after two different enamel bleaching procedures. Forty sound human incisors were randomly assigned to different treatments: bleaching with an in-office agent (Perfect Bleach Office+); bleaching with an at-home agent (Perfect Bleach); bleaching with the in-office agent followed by the prophylaxis paste; bleaching with the at-home agent followed by the prophylaxis paste; no treatment (control). Bleaching was performed at 0, 8, 24 and 32 h, followed by a 3-min re-mineralizing treatment in the subgroups designed to receive it. Specimens underwent a micro-hardness tester and a mean Vickers Hardness number was considered for each specimen. ANOVA exhibited significant differences among groups. Post-hoc Tukey testing showed significant micro-hardness decrease after the application of both the two bleaching agents. The treatment with prophylaxis paste significantly increased the micro-hardness values of bleached enamel.

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Section: Specimen Preparationmentioning

confidence: 99%

In Vitro Re-Hardening of Bleached Enamel Using Mineralizing Pastes: Toward Preventing Bacterial Colonization

et al. 2020

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“…Recently, new toothpastes formulations have been introduced to contrast enamel and dentine erosion (Ganss et al, 2013). Among the large amount of commercially available products, newly introduced toothpastes were subject of our previous studies (Poggio et al, 2013;Lombardini et al, 2014). P 11 -4 is a rationally designed self-assembling peptide (Aggeli et al, '97;Kyle et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Effect of self‐assembling peptide P₁₁‐4 on enamel erosion: AFM and SEM studies

et al. 2015

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The aim of the present in vitro study was to evaluate the protective effect of self-assembling peptide P11 -4 (Curodont™ Protect/Credentis) on enamel erosion produced by a soft-drink, by using Atomic Force Microscopy (AFM) and Scanning Electron Microscopy (SEM). Thirty human incisors were equally and randomly assigned to 6 groups. Group 1: intact enamel, group 2: soft drink, group 3: Curodont(™) Protect applied, group 4: Curodont(™) Protect applied + soft drink, group 5: soft drink + Curodont(™) Protect applied, group 6: soft drink + Curodont(™) Protect applied + soft drink. Specimens were observed through atomic force microscopy (AFM). The root mean-square roughness (Rrms) was obtained from the AFM images and the differences in the averaged values among the groups were analyzed by Shapiro-Wilk test in order to assess the normality of the distribution. Parametric ANOVA and post hoc Tuckey test were performed to assess the differences between the different groups. After demineralization process, enamel demonstrated a high degree of surface porosity. This morphological aspect was reflected in the increase of Rrms values. No significant differences (p > 0.05) were reported between intact enamel and enamel only treated with P11 -4 and between eroded enamel and enamel treated with P11 -4 and then demineralized. However significant differences (p < 0.05) were recorded when comparing softened enamel with softened enamel further remineralized with biomimetic self-assembling peptides and enamel treated with the protective paste between two acid attacks. The use of P11 -4 remineralizing may offer a degree of protection from enamel erosion. SCANNING 38:344-351, 2016. © 2015 Wiley Periodicals, Inc.

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“…Recently, new toothpastes formulations have been introduced to contrast enamel and dentin erosion (Ganss et al, 2013). Among the large amount of commercially available products, several newly toothpastes were subject of our previous studies (Poggio et al, 2013;Lombardini et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Protective effect of casein phosphopeptide‐amorphous calcium phosphate on enamel erosion: Atomic force microscopy studies

et al. 2015

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The aim of this study was to investigate the in vitro effect of a casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) paste (GC Tooth Mousse- TM, GC Corporation, Tokyo, Japan) on preventing enamel erosion, by using Atomic Force Microscopy (AFM). 30 human incisors, were equally assigned to 6 groups: intact enamel, enamel + soft drink, enamel + TM, enamel + TM + soft drink, enamel + soft drink + TM, enamel + soft drink + TM + soft drink. Specimens were observed through atomic force microscopy (AFM). The most common topographical parameters were determined, such as the surface roughness (Rrms ). The use of soft drink on intact enamel has roughened the surface of the sample. The application of the CPP-ACP paste on non-treated enamel made the surface smoother. A significant decrease in roughness was seen after remineralization with CPP-ACP paste. Significant differences were recorded when comparing softened enamel with softened enamel remineralized with CPP-ACP paste. Comparing eroded enamel with demineralized/remineralized specimens, the application of a CPP-ACP paste leads to a significant reduction in roughness values. AFM images of enamel surface treated with CPP-ACP resulted in less morphological changes of the tooth substrate when compared with the only eroded enamel surface morphology; thus, indicating that CPP-ACP paste promoted remineralization. Specimens' surface roughness remained similar regardless that the protective agent is used before or after exposure to coke or between two demineralizing cycles. The results confirmed the effectiveness of the CPP-ACP paste on preventing enamel erosion produced by soft drinks.

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Preventive effect of different toothpastes on enamel erosion: AFM and SEM studies

Abstract: the use of new formulation toothpastes can prevent enamel demineralization.

Cited by 34 publications

References 23 publications

In Vitro Re-Hardening of Bleached Enamel Using Mineralizing Pastes: Toward Preventing Bacterial Colonization

In Vitro Re-Hardening of Bleached Enamel Using Mineralizing Pastes: Toward Preventing Bacterial Colonization

Effect of self‐assembling peptide P₁₁‐4 on enamel erosion: AFM and SEM studies

Protective effect of casein phosphopeptide‐amorphous calcium phosphate on enamel erosion: Atomic force microscopy studies

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Preventive effect of different toothpastes on enamel erosion: AFM and SEM studies

Abstract: the use of new formulation toothpastes can prevent enamel demineralization.

Cited by 34 publications

References 23 publications

In Vitro Re-Hardening of Bleached Enamel Using Mineralizing Pastes: Toward Preventing Bacterial Colonization

In Vitro Re-Hardening of Bleached Enamel Using Mineralizing Pastes: Toward Preventing Bacterial Colonization

Effect of self‐assembling peptide P11‐4 on enamel erosion: AFM and SEM studies

Protective effect of casein phosphopeptide‐amorphous calcium phosphate on enamel erosion: Atomic force microscopy studies

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Effect of self‐assembling peptide P₁₁‐4 on enamel erosion: AFM and SEM studies