Preventive Effects of Cladosiphon Fucoidan Against <i>Helicobacter pylori</i> Infection in Mongolian gerbils

Shibata, Hideyuki; Iimuro, Masaki; Uchiya, Naoaki; Kawamori, Toshihiko; Nagaoka, Masato; Ueyama, Sadao; Hashimoto, Shusuke; Yokokura, Teruo; Sügimura, Takashi; Wakabayashi, Keiji

doi:10.1046/j.1523-5378.2003.00124.x

Cited by 122 publications

(76 citation statements)

References 23 publications

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“…Goni et al (2001) suggested that algal fibres such as fucoidans were resistant to bacterial metabolism. Fucoidans from different species of brown algae have been shown to inhibit the attachment of certain bacterial species in the porcine gastro-intestinal tract as well as preventing the binding of enterococci and streptococci species to the extracellular matrix proteins of animal cells (Shibata et al, 2003), indicating that the fucoidans may be acting as a powerful antimicrobial in reducing the microbial population. Secondly, the non-selective reduction in the microbial populations may be due to the agglutination properties of the extract.…”

Section: Discussionmentioning

confidence: 99%

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The effects of seaweed extract inclusion on gut morphology, selected intestinal microbiota, nutrient digestibility, volatile fatty acid concentrations and the immune status of the weaned pig

Reilly

O’Doherty

Pierce

et al. 2008

Animal

126

105

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An experiment (complete randomised design) was conducted to investigate the effects of Laminaria hyperborea and Laminaria digitata seaweed extract inclusion on gut morphology, selected intestinal microbiota populations, volatile fatty acid concentrations and the immune status of the weaned pig. Twenty-eight piglets (24 days of age, 6.5 6 1.4 kg live weight) were assigned to one of four dietary treatments for 7 days and then sacrificed: (T1) basal diet (control); (T2) basal diet and 1.5 g/kg L. hyperborea seaweed extract; (T3) basal diet and 1.5 g/kg L. digitata seaweed extract; and (T4) basal diet and 1.5 g/kg of a combination of L. hyperborea and L. digitata seaweed extract. The seaweed extract contained both laminarin and fucoidan. Digesta samples were taken from the caecum and colon to measure the enterobacteria, bifidobacteria and lactobacilli populations and for volatile fatty acid analysis. Tissue samples were taken from the duodenum, jejunum and ileum for morphological examination. Blood samples were taken to determine the cytokine gene expression profile and to measure the phagocytotic capacity of the blood. Pigs offered diets containing L. hyperborea seaweed extract had less bifidobacteria in the colon (P , 0.05) and lactobacilli in the caecum (P , 0.05) and colon (P , 0.001). The inclusion of L. digitata seaweed extract resulted in lower populations of enterobacteria in the caecum and colon (P , 0.01), bifidobacteria in the caecum (P , 0.05), and lactobacilli in the caecum (P , 0.05) and colon (P , 0.001). Pigs offered the combination of L. hyperborea and L. digitata seaweed extracts had less enterobacteria (P , 0.05) and lactobacilli (P , 0.01) in the caecum and colon. Pigs offered the L. digitata-supplemented diet had a reduced villous height in the duodenum and jejunum (P , 0.05). The inclusion of the L. digitata seaweed extract increased the molar proportion of butyric acid in the colon (P , 0.05). There was a significant reduction in the ammonia concentration in the colon with the inclusion of L. hyperborea (P , 0.01) and L. digitata (P , 0.05) seaweed extracts. An increase in the expression of the Interleukin-8 mRNA was observed on day 6 with the supplementation of the combination of L. hyperborea and L. digitata seaweed extract (P , 0.05). The inclusion of L. hyperborea seaweed extract resulted in an increase in total monocyte number (P , 0.05). In conclusion, the supplementation of L. hyperborea and L. digitata seaweed extract alone and in combination reduced the enterobacteria, bifidobacteria and lactobacilli populations in the caecum and colon, while only marginal effects on the immune response was observed.

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Section: Discussionmentioning

confidence: 99%

“…They are known to have antibacterial properties as they have been shown to have the ability to inhibit the attachment of certain bacterial species to porcine gastric mucin in vitro (Shibata et al, 2003) as well as having antiviral and anti-inflammatory properties (Marais and Joseleau, 2001).…”

Section: Introductionmentioning

confidence: 99%

The effects of seaweed extract inclusion on gut morphology, selected intestinal microbiota, nutrient digestibility, volatile fatty acid concentrations and the immune status of the weaned pig

Reilly

O’Doherty

Pierce

et al. 2008

Animal

126

105

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“…Fucoidans, the family of sulphated heteropolysaccharides, are composed mainly of a-(1-3) linked L-fucose resides (Percival and McDowell, 1967;Gahan et al, 2009). They are known to have antibacterial properties as they have the ability to inhibit the attachment of certain bacterial species to porcine gastric mucin in vitro (Shibata et al, 2003), as well as having antiviral and anti-inflammatory properties (Marais and Joseleau, 2001;Reilly et al, 2008). …”

Section: Introductionmentioning

confidence: 99%

“…Goñ i et al (2001) suggested that certain algal fibres such as fucoidans were resistant to bacterial metabolism. Fucoidans from different species of brown algae inhibit the attachment of certain bacterial species in the porcine GIT, as well as preventing the binding of enterococci and streptococci species to the extracellular matrix proteins of animal cells (Shibata et al, 2003). This indicates that a component of the LH b-glucan behaves as a greater antimicrobial agent than LD b-glucan.…”

mentioning

confidence: 99%

Analysis of bacterial community shifts in the gastrointestinal tract of pigs fed diets supplemented with β-glucan from Laminaria digitata, Laminaria hyperborea and Saccharomyces cerevisiae

Murphy

Bello

O’Doherty

et al. 2013

Animal

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This study was designed to evaluate the effects of algal and yeast b-glucans on the porcine gastrointestinal microbiota, specifically the community of Lactobacillus, Bifidobacterium and coliforms. A total of 48 pigs were fed four diets over a 28-day period to determine the effect that each had on these communities. The control diet consisted of wheat and soya bean meal. The remaining three diets contained wheat and soya bean meal supplemented with b-glucan at 250 g/tonne from Laminaria digitata, Laminaria hyperborea or Saccharomyces cerevisiae. Faecal samples were collected from animals before feeding each diet and after the feeding period. The animals were slaughtered the following day and samples were collected from the stomach, ileum, caecum, proximal colon and distal colon. Alterations in Lactobacillus in the gastrointestinal tract (GIT) were analysed using denaturing gradient gel electrophoresis (DGGE) profiles generated by group-specific 16S rRNA gene PCR amplicons. Plate count analysis was also performed to quantify total coliforms. DGGE profiles indicated that all b-glucan diets provoked the emergence of a richer community of Lactobacillus. The richest community of lactobacilli emerged after feeding L. digitata (LD b-glucan). Plate count analysis revealed that the L. hyperborea (LH b-glucan) diet had a statistically significant effect on the coliform counts in the proximal colon in comparison with the control diet. b-glucan from L. digitata and S. cerevisiae also generally reduced coliforms but to a lesser extent. Nevertheless, the b-glucan diets did not significantly reduce levels of Lactobacillus or Bifidobacterium. DGGE analysis of GIT samples indicated that the three b-glucan diets generally promoted the establishment of a more varied range of Lactobacillus species in the caecum, proximal and distal colon. The LH b-glucan had the most profound reducing effect on coliform counts when compared with the control diet and diets supplemented with L. digitata and S. cerevisiae b-glucans.Keywords: b-glucans, pigs, algae, yeast, denaturing gradient gel electrophoresis ImplicationsAdministration of antibiotics to animals has come under intense scrutiny because of their contribution to the dissemination of antibiotic-resistant bacteria into the food chain. Algal and yeast b-glucans are biologically active resources from nature. With respect to animal production, an important goal is to determine the optimal microbiota for the animal and then be able to manipulate the microbiota through the diet, supplements, etc. to obtain the desired microbiota. b-glucans derived from macroalgae and yeast aid in gut health during periods of intestinal imbalance, leading to the alteration in the microbial community composition and hence offering an easily accessible alternative to antibiotics. This paper focuses on the use of a molecular and a cultivation-based method to monitor fluctuations in the gastrointestinal microbiota caused by algal and yeast b-glucan supplementation of the pig diet. Molecular analysis of the Lactobaci...

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“…Many medicinal plants have long been applied as remedies to cure such diseases. Euphorbia decipiens (Ahmad et al, 2003) and sulfated polysaccharide of brown seaweed are examples of natural substances reported to have urease inhibition activity (Limuro and Wakabayashi, 2003). The data on enzyme inhibitory activity clearly show the potential of S. pecten-veneris as an active inhibitor of urease and hence suggest that it could be effective in the treatment of gastrointestinal disorders.…”

Section: Enzyme Inhibitionmentioning

confidence: 98%

Phytochemical composition, biological potential and enzyme inhibition activity of Scandix pecten-veneris L.

Wahab

Jan

Rehman

et al. 2018

J. Zhejiang Univ. Sci. B

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Abstract:Objective: Scandix pecten-veneris L. is a less studied wild edible herb and is considered an extinct plant species in many parts of the world. This study was designed to evaluate its phytochemical composition and biological potential of S. pecten-veneris L. Methods: Phytochemicals including alkaloids, flavonoids, polyphenols, and tannins were determined in extracts of S. pecten-veneris. Antioxidant activity was determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH), while reducing power was tested by ferric reducing/antioxidant power (FRAP) assay. Antimicrobial activity against seven bacterial and four fungal strains was evaluated using agar well diffusion assay. Enzymes inhibition study was performed for urease, phosphodiesterase-I, and catalase-II. Results: S. pecten-veneris showed moderate antiradical activity and reducing potential of hydroxyl radicals to about 20% of the initial value. The antioxidant activity of various extracts of S. pecten-veneris showed a linear correlation with total phenolic contents in the order of water>n-butanol>chloroform>ethyl acetate>methanol extracts. S. pecten-veneris leaves showed the highest inhibitory activity against Staphylococcus aureus while the highest antifungal activity was observed against Candida albicans. The plant extract was most potent against urease enzymes but showed moderate activity against phosphodiestrase-I and carbonic anhydrase-II. Conclusions: Our data demonstrate that in addition to its culinary uses, S. pecten-veneris has good medicinal potential and hence could be used for treating some specific health ailments.

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Preventive Effects of Cladosiphon Fucoidan Against Helicobacter pylori Infection in Mongolian gerbils

Abstract: Cladosiphon fucoidan may deserve particular attention as a safe agent that can prevent H. pylori infection and reduce the risk of associated gastric cancer.

Cited by 122 publications

References 23 publications

The effects of seaweed extract inclusion on gut morphology, selected intestinal microbiota, nutrient digestibility, volatile fatty acid concentrations and the immune status of the weaned pig

The effects of seaweed extract inclusion on gut morphology, selected intestinal microbiota, nutrient digestibility, volatile fatty acid concentrations and the immune status of the weaned pig

Analysis of bacterial community shifts in the gastrointestinal tract of pigs fed diets supplemented with β-glucan from Laminaria digitata, Laminaria hyperborea and Saccharomyces cerevisiae

Phytochemical composition, biological potential and enzyme inhibition activity of Scandix pecten-veneris L.

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