Prey DNA detection success following digestion by intraguild predators: influence of prey and predator species

Gagnon, Annie‐Ève; Doyon, Josée; Heimpel, George E.; Brodeur, Jacques

doi:10.1111/j.1755-0998.2011.03047.x

Cited by 59 publications

(68 citation statements)

References 80 publications

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“…Because of the difficulty of analyzing gut content, especially for small arthropods, very few quantitative data are available for gut capacity and digestion time of insects. However, PCRbased gut content analysis in ladybeetles revealed that prey DNA detection time, an indirect measure of digestion time, varied from 1 to 19 h depending on the predator species and prey species and size (Hoogendoorn and Heimpel 2001, McMillan et al 2007, Gagnon et al 2011. Our estimates of the corrected digestion FIG.…”

Section: Discussionmentioning

confidence: 85%

Parsing handling time into its components: implications for responses to a temperature gradient

Sentis

Hemptinne

Brodeur

2013

Ecology

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Abstract. The functional response is a key element of predator-prey interactions, and variations in its parameters influence interaction strength and population dynamics. Recent studies have used the equation of the metabolic theory of ecology (MTE) to quantify the effect of temperature on the parameter Th, called ''handling time,'' and then predict the responses of predators and communities to climate change. However, our understanding of the processes behind Th and how they vary with temperature remains limited. Using a ladybeetle-aphid system, we compared estimates of Th to direct observations of handling time across a temperature gradient. We found estimated Th values to be greater than observed Th values, suggesting that predation rate is not limited by the time available for handling prey. We next estimated the corrected digestion time, i.e., digestion time corrected for gut capacity, by subtracting observed to estimated Th values. We finally plotted the relationships between temperature and handling or digestion rates. As predicted by MTE, the corrected digestion rate increased exponentially with warming whereas, in contrast to MTE prediction, the relationship between handling rate and temperature was hump shaped. The parameter Th is thus confusing because it combines handling and digestive processes that have different thermal responses. This may explain why general patterns in the relationship between Th and temperature have been difficult to identify in previous studies.

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Section: Discussionmentioning

confidence: 85%

Parsing handling time into its components: implications for responses to a temperature gradient

Sentis

Hemptinne

Brodeur

2013

Ecology

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“…H. axyridis also practices indirect intraguild predation on aphid parasitoids, because it preferentially consumes parasitized aphids over uninfected ones . Laboratory and field studies of intraguild predation involve both visual observations and, more recently, the screening of gut contents by DNA analysis (e.g., Gagnon et al, 2011;Rondoni et al, 2015). Such studies have repeatedly indicated that intraguild predation behavior is important for the invasion success of H. axyridis.…”

Section: Intraguild Predationmentioning

confidence: 99%

Behavioral and Immunological Features Promoting the Invasive Performance of the Harlequin Ladybird Harmonia axyridis

2017

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The harlequin ladybird Harmonia axyridis is now established as a model to test hypotheses explaining why some species become successfully invasive, while others, even closely related ones, do not. In this review, we evaluate behavioral and immunological features that may play a role in the invasive performance of this model species. We discuss the behavioral traits and associated semiochemicals that promote the invasive success of H. axyridis. In particular, we consider (1) the aggregative behavior and the particular role of long-chain hydrocarbons; (2) the importance of sex pheromones and non-volatile chemicals in mate location and selection; (3) the use of allelochemicals for prey location; and (4) the nature of chemicals that protect against natural enemies. We also highlight the superior immune system of H. axyridis, which encompasses a broader spectrum of antimicrobial peptides (and higher inducible expression levels) compared with native ladybird beetles such as Adalia bipunctata and Coccinella septempunctata. The chemical defense compound harmonine and the antimicrobial peptides are thought to confer resistance against the abundant microsporidia carried by H. axyridis. These parasites can infect and kill native ladybird species feeding on H. axyridis eggs or larvae, supporting the hypothesis that intraguild predation plays a role in the ability of H. axyridis to outcompete native ladybird species in newly-colonized areas.

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“…This was confirmed by Gagnon et al (2011a), who used this method to investigate IGP interactions between different ladybirds, including H. axyridis. Despite close taxonomic relations between the different predators and prey, the half-life of the period for which the DNA of the prey could be detected recorded in the latter study ranged from 5.2 to 19.3 h. In our study, the level of detection of the DNA of prey also differed for the different species of prey, supporting the conclusion of McMillan et al (2007) and Gagnon et al (2011a) that detection times cannot be generalized even if predators and prey are closely related and DNA fragments from the same region and of similar length are targeted.…”

Section: Discussionmentioning

confidence: 63%

“…Agusti et al, 2003;Hosseini et al, 2008). The half-life of the period for which prey DNA could be detected for each predator-prey combination ranged from 8.9 to 52.4 h; the long half-life for fourth instar H. axyridis feeding on C. carnea indicates it is possible to detect relatively rare predation events (Gagnon et al, 2011a). However, long detection periods could obscure the frequency of predatory events in the field, since recent feeding events are not distinguishable from older ones, and could lead to overestimation of predation rates.…”

Section: Discussionmentioning

confidence: 99%

Molecular analysis of the gut contents of Harmonia axyridis (Coleoptera: Coccinellidae) as a method for detecting intra-guild predation by this species on aphidophagous predators other than coccinellids

Ingels¹,

Aebi²,

Hautier³

et al. 2013

Eur. J. Entomol.

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Abstract. Several studies have demonstrated that the invasive ladybird Harmonia axyridis is a strong intra-guild predator of native species of ladybird. Laboratory studies have shown that H. axyridis can be an intra-guild predator of aphid predators other than coccinellids, including the hoverfly Episyrphus balteatus and lacewing Chrysoperla carnea. However, little is known about the effect of intra-guild predation (IGP) by H. axyridis on hoverfly and lacewing populations in the field. In the present study molecular analyses were used to detect the DNA of E. balteatus and C. carnea in the gut contents of H. axyridis. Primers for the syrphid and chrysopid prey were designed and feeding experiments performed to determine how long prey DNA remains detectable in the guts of this ladybird. DNA detection was influenced by the life stage of the predator and species of prey. Meal size did not affect detection time, except when fourth instar individuals of H. axyridis were fed 10 eggs or one second instar of C. carnea. Predator weight, sex and morpho-type (melanic/non-melanic) did not influence DNA detection. The half-life of the time for which the DNA of the prey remained detectable was calculated for each predator-prey combination, and ranged from 8.9 to 52.4 h. This method can be used to study the ecological importance of IGP by H. axyridis on aphidophagous predators other than coccinellids in the field.

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Prey DNA detection success following digestion by intraguild predators: influence of prey and predator species

Cited by 59 publications

References 80 publications

Parsing handling time into its components: implications for responses to a temperature gradient

Parsing handling time into its components: implications for responses to a temperature gradient

Behavioral and Immunological Features Promoting the Invasive Performance of the Harlequin Ladybird Harmonia axyridis

Molecular analysis of the gut contents of Harmonia axyridis (Coleoptera: Coccinellidae) as a method for detecting intra-guild predation by this species on aphidophagous predators other than coccinellids

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