PRIGo: a new multi-axis goniometer for macromolecular crystallography

Waltersperger, S.; Oliéric, V.; Pradervand, Claude; Glettig, Wayne; Salathe, M.; Fuchs, Martin R.; Curtin, Adrian; Wang, Xiaoqiang; Ebner, Simon Gregor; Panepucci, Ezequiel; Weinert, T.; Schulze-Briese, Clemens; Wang, Meitian

doi:10.1107/s1600577515005354

Cited by 53 publications

(39 citation statements)

References 24 publications

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“…On a single crystal, 4 × 360° data sets were collected at 100 K at a wavelength of 2.075 Å with 0.1° oscillation 0.1 s exposure in four different orientations of a multi‐axis goniometer (Waltersperger et al , 2015), as previously described (Weinert et al , 2015). The sample‐to‐detector distance was set to 120 mm.…”

Section: Methodsmentioning

confidence: 99%

Sen1 has unique structural features grafted on the architecture of the Upf1‐like helicase family

et al. 2017

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The superfamily 1B (SF1B) helicase Sen1 is an essential protein that plays a key role in the termination of non‐coding transcription in yeast. Here, we identified the ~90 kDa helicase core of Saccharomyces cerevisiae Sen1 as sufficient for transcription termination in vitro and determined the corresponding structure at 1.8 Å resolution. In addition to the catalytic and auxiliary subdomains characteristic of the SF1B family, Sen1 has a distinct and evolutionarily conserved structural feature that “braces” the helicase core. Comparative structural analyses indicate that the “brace” is essential in shaping a favorable conformation for RNA binding and unwinding. We also show that subdomain 1C (the “prong”) is an essential element for 5′‐3′ unwinding and for Sen1‐mediated transcription termination in vitro. Finally, yeast Sen1 mutant proteins mimicking the disease forms of the human orthologue, senataxin, show lower capacity of RNA unwinding and impairment of transcription termination in vitro. The combined biochemical and structural data thus provide a molecular model for the specificity of Sen1 in transcription termination and more generally for the unwinding mechanism of 5′‐3′ helicases.

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Section: Methodsmentioning

confidence: 99%

Sen1 has unique structural features grafted on the architecture of the Upf1‐like helicase family

et al. 2017

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“…The detector was vertically translated by 67 mm to increase the maximum resolution to 0.67 Å. The X-ray diffraction data were collected from a single crystal mounted onto the multi-axis PRIGo goniometer [37] using the DA+ acquisition software [38]. Data were collected at the [39].…”

Section: Methodsmentioning

confidence: 99%

Sharyginite, Ca3TiFe2O8, A New Mineral from the Bellerberg Volcano, Germany

et al. 2018

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Abstract:The new mineral sharyginite, Ca 3 TiFe 2 O 8 (P2 1 ma, Z = 2, a = 5.423(2) Å, b = 11.150(8) Å, c = 5.528(2) Å, V = 334.3(3) Å 3 ), a member of the anion deficient perovskite group, was discovered in metacarbonate xenoliths in alkali basalt from the Caspar quarry, Bellerberg volcano, Eifel, Germany. In the holotype specimen, sharyginite is widespread in the contact zone of xenolith with alkali basalt. Sharyginite is associated with fluorellestadite, cuspidine, brownmillerite, rondorfite, larnite and minerals of the chlormayenite-wadalite series. The mineral usually forms flat crystals up to 100 µm in length, which are formed by pinacoids {100}, {010} and {001}. Crystals are flattened on (010). Sharyginite is dark brown, opaque with a brown streak and has a sub-metallic lustre. In reflected light, it is light grey and exhibits rare yellowish-brown internal reflections. The calculated density of sharyginite is 3.943 g·cm -3 . The empirical formula calculated on the basis of 8 O apfu is Ca 3.00 (Fe 3+ octahedra, which are separated by single layers of (Fe 3+ O 4 ) tetrahedra. We suggest that sharyginite formed after perovskite at high-temperature conditions >1000 • C.

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“…High multiplicity achieved at reduced dose in multiple orientation has proved very effective for achieving satisfactory signal-to-noise [18]. This approach requires use of a multi-axis goniometer such as PRIGo [19]. When radiation damage is a concern, data from different crystals may be used to improve data quality [20,21].…”

Section: Routine Procedures For Sad Phasingmentioning

confidence: 99%

Contemporary Use of Anomalous Diffraction in Biomolecular Structure Analysis

Hendrickson

2017

Methods in Molecular Biology

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The normal elastic X-ray scattering that depends only on electron density can be modulated by an ‘anomalous’ component due to resonance between X-rays and electronic orbitals. Anomalous scattering thereby precisely identifies atomic species, since orbitals distinguish atomic elements, which enables the multi- and single-wavelength anomalous diffraction (MAD and SAD) methods. SAD now predominates in de novo structure determination of biological macromolecules, and we focus here on the prevailing SAD method. We describe the anomalous phasing theory and the periodic table of phasing elements that are available for SAD experiments, differentiating between those readily accessible for at-resonance experiments and those that can be effective away from an edge. We describe procedures for present-day SAD phasing experiments and we discuss optimization of anomalous signals for challenging applications. We also describe methods for using anomalous signals as molecular markers for tracing and element identification. Emerging developments and perspectives are discussed in brief.

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PRIGo: a new multi-axis goniometer for macromolecular crystallography

Cited by 53 publications

References 24 publications

Sen1 has unique structural features grafted on the architecture of the Upf1‐like helicase family

Sen1 has unique structural features grafted on the architecture of the Upf1‐like helicase family

Sharyginite, Ca3TiFe2O8, A New Mineral from the Bellerberg Volcano, Germany

Contemporary Use of Anomalous Diffraction in Biomolecular Structure Analysis

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