Primary and secondary somatic embryogenesis in Chrysanthemum cv. Euro

Abstract

Somatic embryogenesis was induced from in vivo grown leaf explants of Chrysanthemum cv. Euro incubated on Murashige and Skoog (MS) medium supplemented with 2.0 mg/L 2,4-dichlorophenoxyacetic acid and 2.0 mg/L Kinetin, yielding the highest mean number of embryos (42 ± 5.97) per explant after 5 weeks of culture. We evaluated the effects of basal medium, various concentrations of sucrose, and timentin on the proliferation of secondary somatic embryos. MS medium was observed to be the more effective in promoting the proliferation of somatic embryos than half-strength Murashige and Skoog (1/2MS). In addition, timentin was also more efficient in induction of secondary embryogenesis than sucrose. Whole plantlets were obtained by culturing of secondary embryos on hormone-free MS medium and successfully acclimated in the green house.

High frequency plant regeneration in Indian Siris via cyclic somatic embryogenesis with biochemical, histological and SEM investigations

“…Here it could be noticed that the two different media (WPM and MS) were required for the induction and maturation of PSEs while induction and maturation of SSEs occurred on the MS medium only. Contrary to our finding, generally the same kind of media is found to be suitable for the induction of both primary as well as secondary somatic embryogenesis (Naing et al, 2013). It may be concluded that in A. lebbeck nutrient composition of WPM (low NH 4 + and NO 3 contents; high SO 4 2 concentration) is required for the onset of embryogenesis but long term maintenance of embryogenic competence was achieved with the use of MS formulations (higher concentrations of NH 4 + and NO 3 and low SO 4 2 content).…”

“…Similar to our finding, Mithila et al (2003) reported somatic embryogenesis at higher TDZ doses. Naing et al (2013) also reported KN to be the best PGR for the enhancement in the number of somatic embryoids induction per explant followed by BA and TDZ. KN also proved to be highly efficient for callus proliferation and subsequent differentiation of embryoids from leaf explants of cumin (Tawfik and Noga, 2002).…”

Thidiazuron Induces High Frequency Direct Somatic Embryogenesis Growth from Cotyledon Culture of Eurycoma longifolia

“…However, combination with IBA exhibited a reasonable number of somatic embryos (Table 3). Naing et al (2012) also reported that no formation of embryo from leaf explants of Chrysanthemum cv. Euro when cultured on the medium containing TDZ alone, but combination with 2,4-D produced secondary embryos growth.…”

Genetic Diversity of Chrysanthemum Plants Derived via Somatic Embryogenesis Using Rapd Markers

“…This is possible by applying two methods: adventitious shoots [Broertjes et al 1976, Jerzy and Zalewska 1997, Song et al 2011, Teixeira da Silva et al 2015 and somatic embryogenesis [Lema-Rumińska and Śliwińska 2015]. These methods involve growth regulators; in addition, the regeneration often occurs indirectly via callus [Zalewska et al 2011, Xu et al 2012, Naing et al 2013a, b, Lema-Rumińska and Niedojadło 2014. As a consequence, the resulting plants can be unstable [Miler and Zalewska 2014].…”