Primary and secondary somatic embryogenesis in Chrysanthemum (Chrysanthemum morifolium) cv. ‘Baeksun’ and assessment of ploidy stability of somatic embryogenesis process by flow cytometry

Naing, Aung Htay; Min, Jeon Su; Park, Kyung‐Il; Chung, Mi Young; Lim, Sun Hyung; Lim, Ki Byung; Kim, Chang Kil

doi:10.1007/s11738-013-1328-4

Cited by 18 publications

(6 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…7 Flow cytometry histograms of Taraxacum pieninicum after 9 months of in vitro storage at low temperature in magenta boxes: control plant (without storage at low temperature) (a), in light conditions on medium without ABA (b), in light conditions on medium with ABA (c), in darkness on medium without ABA (d), in darkness on medium with ABA (e) et al 1997; Kanchanapoom et al 2012;Adhikari et al 2014). Similarly indirect somatic embryogenesis from leaf explants of Chrysanthemum morifolium (Naing et al 2013) and Gentiana species (Fiuk and Rybczyński 2008), the plants showed the same ploidy level as donor plants. In our study all the regenerants from material stored at reduced temperature and regrown in optimal conditions had the same ploidy level, irrespective of the storage treatment.…”

Section: Discussionmentioning

confidence: 99%

Effect of light conditions and ABA on cold storage and post-storage propagation of Taraxacum pieninicum

Kamińska

Skrzypek

Wilmowicz

et al. 2016

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

Taraxacum pieninicum is an extremely threatened species. It seems that traditional protection methods of this species are insufficient and it is necessary to use biotechnology tools that allow the in vitro storage of plant material. The present study describes in vitro conservation of T. pieninicum by slow-growth storage. Various light conditions and abscisic acid (ABA) treatments and combinations thereof were tested. Viability, proliferation rate and ability of shoots to root were evaluated during regrowth. Moreover, the effect of shoot storage conditions on the ABA level was analysed. The results showed that light during 3-months' storage increased the level of endogenous ABA. Similar results were observed when storage at a low temperature was prolonged to 9 months. Changes in ABA level had a negative effect on shoot condition. However, these changes were related to leaves and did not affect the ability of shoot tips to proliferate after long-term storage. Addition of ABA to storage medium increased several folds the level of ABA in plant tissues, which resulted in a reduction in the visual rating and proliferation rate. Shoots obtained after post-storage regrowth were able to root. All rooted shoots survived adaptation to field conditions and were able to flower in the second year after acclimatization. The analysis of DNA content indicated that all the regenerants had the same ploidy level independent of treatments during storage. Cold storage of T. pieninicum as used in this study enabled the interval between subcultures to be extended to 9 months.

show abstract

Section: Discussionmentioning

confidence: 99%

Effect of light conditions and ABA on cold storage and post-storage propagation of Taraxacum pieninicum

Kamińska

Skrzypek

Wilmowicz

et al. 2016

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

show abstract

“…polyploidization). Similarly, no ploidy changes were detected in chrysanthemums produced via somatic embryogenesis, which grew either in MS PGR-free or supplemented with PGRs media (Lema-Rumińska and Sliwinska 2009Sliwinska , 2015Naing et al 2013). However, in those reports only relative DNA content/DNA ploidy was established, which did not allow the detection of smaller than genomic changes.…”

Section: In Vitro Shoot Development and Genome Size Variation Of Radimentioning

confidence: 97%

Nuclear DNA content as an indicator of inflorescence colour stability of in vitro propagated solid and chimera mutants of chrysanthemum

Miler

Kulus

Śliwińska

2020

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

In chrysanthemum, breeders seek for desirable characteristics of the inflorescence, which can first be established once the plant is mature. The present study aims to determine whether measurement of DNA content can be useful in the detection of somaclonal variants and/or separation of chimera components in chrysanthemum at the early in vitro multiplication stage. Eleven Chrysanthemum × morifolium (Ramat.) Hemsl. cultivars of the Lady group (a mother cultivar and ten of its radiomutants obtained by X-ray- or γ-irradiation; solid and periclinal chimeras) were propagated in vitro. Single-node explants were cultured in Murashige and Skoog (MS) medium, either without plant growth regulators (PGRs) or supplemented with 6-benzyladenine (BA) and indole-3-acetic acid (IAA). The nuclear DNA content was measured by flow cytometry (FCM) in the shoots produced in vitro. After acclimatization and growth of the plants in a glasshouse, inflorescence colour was recorded. The addition of PGRs to the medium almost doubled the mean number of shoots produced in vitro per explant, but caused a change in inflorescence colour of all (‘Lady Apricot’; periclinal chimera) or part of the plants (‘Lady Amber’; solid mutant and ‘Lady Salmon’; periclinal chimera). All radiomutants contained less DNA than the mother cultivar ‘Richmond’. There were significant differences in DNA content between plants of the same cultivar grown in media with or without PGRs for ‘Lady Apricot’ and ‘Lady Salmon’, but no phenotype alternation occurred in chrysanthemums produced in PGR-free medium compared to the original cultivars. Conversely, in medium with PGRs, chimeras produced flowers different from the original colour. In all except one cultivar (‘Lady Amber’; solid mutant) a lack of differences in genome size between plants grown in either medium coincided with a stable inflorescence colour. The occurrence of some plants of ‘Lady Amber’ with different inflorescence colour may be due to small DNA changes, undetectable by FCM. It can be concluded that FCM analysis of DNA content in young plantlets can be indicative of the stability of inflorescence colour in chrysanthemum, especially chimeric cultivars, and for mutant detection.

show abstract

“…Plant regeneration with chrysanthemum via somatic embryogenesis gives higher efficiency than adventitious organogenesis [Zalewska et al 2007]. Somatic embryogenesis in chrysanthemum is induced on MS medium [Murashige and Skoog 1962] with addition of auxin -2,4--dichlorophenoxyacetic acid (2,4-D) or α-naphthylacetic acid (NAA) as well as cytokinins: 6-benzylaminopurine (BAP) or kinetin (KIN), and generally this process occurs indirectly, via callus [Naing et al 2013, Lema--Rumińska and Śliwińska 2015. The presence of growth regulators can threatens the genetic stability and can lead to somaclonal variation [Miler and Zalewska 2014].…”

Section: Introductionmentioning

confidence: 99%

ISSR markers in the genetic diversity of chrysanthemum plants derived via somatic embryogenesis

Michałowska

Lema-Rumińska

2018

Zeszyty Problemowe Postępów Nauk Rolniczych

View full text Add to dashboard Cite

show abstract

Primary and secondary somatic embryogenesis in Chrysanthemum (Chrysanthemum morifolium) cv. ‘Baeksun’ and assessment of ploidy stability of somatic embryogenesis process by flow cytometry

Cited by 18 publications

References 32 publications

Effect of light conditions and ABA on cold storage and post-storage propagation of Taraxacum pieninicum

Effect of light conditions and ABA on cold storage and post-storage propagation of Taraxacum pieninicum

Nuclear DNA content as an indicator of inflorescence colour stability of in vitro propagated solid and chimera mutants of chrysanthemum

ISSR markers in the genetic diversity of chrysanthemum plants derived via somatic embryogenesis

Contact Info

Product

Resources

About