Primary cell culture and morphological characterization of canine dermal papilla cells and dermal fibroblasts

Bratka‐Robia, Christine B.; Mitteregger, Gerda; Aichinger, Amanda; Egerbacher, Monika; Helmreich, M.; Bamberg, E.

doi:10.1046/j.0959-4493.2001.00276.x

Cited by 29 publications

(44 citation statements)

References 26 publications

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“…These data suggest that DPLTs may have hair inducing activity. Consistent with our findings, the levels of versican and ALP are closely associated with in vivo hair inducing activity of the DPCs [6, 11, 12], and a distinct tendency to form cell aggregates of dermal papilla cells indirectly indicates the in vivo trichogenic property of cells [2, 8, 20]. …”

Section: Discussionsupporting

confidence: 88%

Hair growth promoting effect of dermal papilla like tissues from canine adipose-derived mesenchymal stem cells through vascular endothelial growth factor

Lee

Bae

Lee

et al. 2016

The Journal of Veterinary Medical Science

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The purpose of this study was to investigate the protein expression pattern and the in vivo trichogenicity of dermal papilla-like tissues (DPLTs) made from canine adipose-derived mesenchymal stem cells (ASCs) in athymic nude mice. Canine ASCs were isolated and cultured from adipose tissue, and differentiation was induced by culturing ASCs in dermal papilla forming media. DPLTs were embedded in collagen gel, and their structural characteristics and protein expression were evaluated by hematoxylin and eosin stain and immunohistochemistry. Athymic nude mice were divided into two groups (control and DPLTs groups), and DPLTs were injected in skin wounds of mice in the DPLTs group. The trichogenicity of DPLTs was assessed by gross and histological evaluations for 30 days. The fate and the growth factor-secretion effect of DPLTs were evaluated by immunohistochemistry and Western blotting. DPLTs have a compact aggregated structure, form extracellular matrix and highly express the protein specific for dermal papillae, including ALP and versican. New hair follicle formation was remarkable in nude mice of the DPLTs group in gross findings and H&E stain. Vascularization was increased in the DPLTs group, which was the effect of vascular endothelial growth factor secreted by DPLTs in vitro and in vivo. These data suggest that engineered canine DPLTs have characteristics of dermal papillae and have a positive effect on hair regeneration by secreting growth factors.

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Section: Discussionsupporting

confidence: 88%

Hair growth promoting effect of dermal papilla like tissues from canine adipose-derived mesenchymal stem cells through vascular endothelial growth factor

Lee

Bae

Lee

et al. 2016

The Journal of Veterinary Medical Science

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“…Viability of FACS-isolated DP cells was assessed by Trypan Blue (Sigma, St. Louis, Missouri, United States) staining, and equal numbers of live cells (5,000/cm 2 ) were plated in Amniomax C-100 medium (Invitrogen), previously used for dog whisker DP cells [47]. This was the best of the five media tested.…”

Section: Methodsmentioning

confidence: 99%

Molecular Dissection of Mesenchymal–Epithelial Interactions in the Hair Follicle

2005

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De novo hair follicle formation in embryonic skin and new hair growth in adult skin are initiated when specialized mesenchymal dermal papilla (DP) cells send cues to multipotent epithelial stem cells. Subsequently, DP cells are enveloped by epithelial stem cell progeny and other cell types to form a niche orchestrating hair growth. Understanding the general biological principles that govern the mesenchymal–epithelial interactions within the DP niche, however, has been hampered so far by the lack of systematic approaches to dissect the complete molecular make-up of this complex tissue. Here, we take a novel multicolor labeling approach, using cell type–specific transgenic expression of red and green fluorescent proteins in combination with immunolabeling of specific antigens, to isolate pure populations of DP and four of its surrounding cell types: dermal fibroblasts, melanocytes, and two different populations of epithelial progenitors (matrix and outer root sheath cells). By defining their transcriptional profiles, we develop molecular signatures characteristic for the DP and its niche. Validating the functional importance of these signatures is a group of genes linked to hair disorders that have been largely unexplored. Additionally, the DP signature reveals novel signaling and transcription regulators that distinguish them from other cell types. The mesenchymal–epithelial signatures include key factors previously implicated in ectodermal-neural fate determination, as well as a myriad of regulators of bone morphogenetic protein signaling. These findings establish a foundation for future functional analyses of the roles of these genes in hair development. Overall, our strategy illustrates how knowledge of the genes uniquely expressed by each cell type residing in a complex niche can reveal important new insights into the biology of the tissue and its associated disease states.

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“…DPCs with hair-inducing activity tend to aggregate [36]. Rushan et al [34] studied the genes associated with the aggregative property of DPCs using low passage DPCs and high passage DPCs.…”

Section: Rt-pcr Analysis Of Self-aggregated Dpltsmentioning

confidence: 98%

Application of mesenchymal stem cells derived from bone marrow and umbilical cord in human hair multiplication

Yoo

Shin

Yoon

et al. 2010

Journal of Dermatological Science

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Primary cell culture and morphological characterization of canine dermal papilla cells and dermal fibroblasts

Cited by 29 publications

References 26 publications

Hair growth promoting effect of dermal papilla like tissues from canine adipose-derived mesenchymal stem cells through vascular endothelial growth factor

Hair growth promoting effect of dermal papilla like tissues from canine adipose-derived mesenchymal stem cells through vascular endothelial growth factor

Molecular Dissection of Mesenchymal–Epithelial Interactions in the Hair Follicle

Application of mesenchymal stem cells derived from bone marrow and umbilical cord in human hair multiplication

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