Primary insect cell culture from total embryo and embryonic brain tissue of Periplaneta americana: A preliminary study

Abstract

The aim of this preliminary study was to establish a primary insect cell culture from total embryos and embryonic brain tissues of Periplaneta americana, collected from Izmir, Turkey. Cells were cultured at 29?C in Grace?s insect medium for one month. In the embryonic brain tissue culture, single cells and cell clumps containing spherical and ovoid as well as dividing cells were observed. Single bipolar neurons were detected after 4 days in culture. Network systems comprised of bipolar neurons were observed on the 5th day of incubation. In addition, presumably glia cells were observed in the embryonic brain culture. In the total embryo culture, the cell population exhibited variable morphologies, including spherical, spindle-like, polygonal and giant cells after nearly 20 days; the culture covered almost half of the Petri dish area within 30 days. This preliminary study associated with Periplaneta americana primary cell culture is the first of its kind in Turkey. These results should contribute to the development of new insect cell lines that are indigenous to Turkey.

Primary Culture of Lepidopteran Adherent and Suspension Cells from Larval Testes of Bombyx mori

“…Among the invertebrates, insect cell cultures are frequently established from Lepidoptera and Diptera, and various tissues belonging to these insects such as reproductive tissues (ovaries/ testes), hemolymph, imaginal discs, fat bodies, midguts, nervous, endocrine and muscular systems or whole embryo and neonate larvae have been cultured [13].…”

“…Although more than 500 insect cell lines have been established, it is stated that new studies are necessary to find more effective insect cell lines for production of recombinant proteins [12]. Among the invertebrates, insect cell cultures are frequently established from Lepidoptera and Diptera, and various tissues belonging to these insects such as reproductive tissues (ovaries/ testes), hemolymph, imaginal discs, fat bodies, midguts, nervous, endocrine and muscular systems or whole embryo and neonate larvae have been cultured [13].…”