Primary isolated hepatic oval cells maintain progenitor cell phenotypes after two-year prolonged cultivation

Wang, Ping; Ma, Cong; Liu, Tianhui; Yang, Aiting; Cong, Rui; Wu, Peng; Tang, Shuang; Xu, Yong; Wang, Hui; Wang, Bao-en; Jia, Jidong; You, Hong

doi:10.1016/j.jhep.2010.05.014

Cited by 27 publications

(43 citation statements)

References 24 publications

(33 reference statements)

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“…To induce biliary differentiation, WB-F344 cells were treated with 3.75 mM sodium butyrate (Sigma-Aldrich). 49,50 The medium was changed every 2 d. The days of differentiation were numbered consecutively beginning at the first day of the SB treatment (0d) to the last day (5d).…”

Section: Cell Culture and Treatmentsmentioning

confidence: 99%

Autophagy regulates biliary differentiation of hepatic progenitor cells through Notch1 signaling pathway

et al. 2016

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Autophagy plays important roles in self-renewal and differentiation of stem cells. Hepatic progenitor cells (HPCs) are thought to have the ability of self-renewal as well as possess a bipotential capacity, which allows them to differentiate into both hepatocytes and bile ductular cells. However, how autophagy contributes to self-renewal and differentiation of hepatic progenitor cells is not well understood. In this study, we use a well-established rat hepatic progenitor cell lines called WB-F344, which is treated with 3.75 mM sodium butyrate (SB) to promote the differentiation of WB-F344 along the biliary phenotype. We found that autophagy was decreased in the early stage of biliary differentiation, and maintained a low level at the late stage. Activation of autophagy by rapamycin or starvation suppressed the biliary differentiation of WB-F344. Further study reported that autophagy inhibited Notch1 signaling pathway, which contributed to biliary differentiation and morphogenesis. In conclusions, autophagy regulates biliary differentiation of hepatic progenitor cells through Notch1 signaling pathway.

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Section: Cell Culture and Treatmentsmentioning

confidence: 99%

Autophagy regulates biliary differentiation of hepatic progenitor cells through Notch1 signaling pathway

et al. 2016

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“…Under the right conditions, these cells can retain tissue-specific functionality, and therefore demonstrate a repertoire of cellular functions likely to increase the predictive value of data gathered from the cells' use as screening tools, or as models for biomedical research (58,59). This attractive advantage comes, however, with the greater technical challenge of, firstly, making these cells and, if necessary, banking them ready for use and, on the other hand, ensuring that the "right conditions" are found to ensure retention of that advantageous functionality.…”

Section: In Vitro Systems: Primary Cells Vs Immortalised Cellsmentioning

confidence: 99%

Advanced in vitro systems for efficacy and toxicity testing in nanomedicine

Fabbrizi

Duff

Oliver

et al. 2014

European Journal of Nanomedicine

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Abstract:The use of nanoparticles (NPs) in nanomedicine is becoming an established therapeutic strategy for transport and delivery of bioactive molecules across biological barriers to elicit a specific cytological response in a target cell population. The utility of NP-derivatised nanomedicines and their potential therapeutic benefits, together with their biosafety, are being explored in cell models that, in order to accurately predict clinical potential, must reflect the architecture and function of the tissues from which the cells originate. These cell-based models, and their application in preclinical nanomedicine, are the subject of this review. Models are considered expressly from a commercial perspective, for their use as screening tools to identify and optimise therapeutic constructs, rather than as research tools requiring minimal throughput. Commercial utility is discussed in terms of the sourcing and assembly of cell-based model components, likelihood of data outputs highly predictive of clinical performance, and the compromise between need for advanced 3D culture architectures and the incremental difficulty in assembling those structures cost-effectively for mediumthroughput analysis. The relative merits of cell lines and primary human cells are discussed, the latter with respect to their potential physiological relevance when cultured under permissive conditions conferred by a biologicallyrich micro-environment. Tools for assembly of such microenvironments are reviewed, and the practitioner's choices of commercial products enabling 3D cell culture are also critically evaluated, from the merits of cell aggregates and micro-tissues to the architectural attractions of recreating epithelial monolayers and multi-cell type reconstituted tissues. Additionally, pitfalls and practical challenges in co-assembly of cells and scaffolds/matrices are considered, with the intention of signposting routes to consistent, scalable production of 3D cell cultures capable of screening the nanomedical potential of NP-conjugated therapeutics.

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“…Lysates were analyzed by electrophoresis on 15% polyacrylamide gels and transferred to PVDF membranes (Millipore, Bedford, NH). The signal was detected using the ECL system (Pierce Chemical Company, Rockford, IL, USA) and quantified by densitometry as previously described (Wang et al, 2010).…”

Section: Western Blottingmentioning

confidence: 99%

HMGB1 Promotes the Synthesis of Pro-IL-1β and Pro-IL-18 by Activation of p38 MAPK and NF-κB Through Receptors for Advanced Glycation End-products in Macrophages

You²,

Li³

et al. 2012

Asian Pacific Journal of Cancer Prevention

Self Cite

105

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The high mobility group box-1 (HMGB1) protein and NALP3 inflammasome have been identified to play important roles in inflammation and cancer pathogenesis, but the relationships between the two and cancer remain unclear. The current study investigated the relationship between HMGB1 and the NALP3 inflammasome in THP-1 macrophages. HMGB1 was found unable to activate the NALP3 inflammasome and failed to induce the release of the IL-1β and IL-18 in THP-1 macrophages. HMGB1 was also found significantly enhanced the activity of ATP to induce IL-1β and IL-18 by the induction of increased expression of pro-IL-1β and pro-IL-18. This process was dependent on activation of RAGE, MAPK p38 and NF-κB signaling pathway. These results demonstrate that HMGB1 promotes the synthesis of pro-IL-1β and pro-IL-18 in THP-1 macrophages by the activation of p38 MAPK and NF-κB through RAGE. HMGB1 likely plays an important role in the first step of the release of the IL-1β and IL-18, preparing for other cytokines to induce excessive release of IL-1β and IL-18 which promote inflammation and cancer progression.

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Primary isolated hepatic oval cells maintain progenitor cell phenotypes after two-year prolonged cultivation

Cited by 27 publications

References 24 publications

Autophagy regulates biliary differentiation of hepatic progenitor cells through Notch1 signaling pathway

Autophagy regulates biliary differentiation of hepatic progenitor cells through Notch1 signaling pathway

Advanced in vitro systems for efficacy and toxicity testing in nanomedicine

HMGB1 Promotes the Synthesis of Pro-IL-1β and Pro-IL-18 by Activation of p38 MAPK and NF-κB Through Receptors for Advanced Glycation End-products in Macrophages

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