Primary Structure and Effect of pH on the Expression of the Plasma Membrane H+-ATPase from Dunaliella acidophila and Dunaliella salina

Weiss, Martin; Pick, Uri

doi:10.1104/pp.112.4.1693

Cited by 33 publications

(26 citation statements)

References 31 publications

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“…Dunaliella acidophila possesses a wellcharacterized P-type H + ATPase to maintain cytosolic neutrality (Sekler et al, 1991). The H + -ATPase transcripts increase with decreasing extracellular pH (Weiss and Pick, 1996). D. acidophila also contains two contractile vacuoles, but they may not be very active due to the hypersaline environments in which the organism is found.…”

Section: Discussionmentioning

confidence: 99%

“…V ATP can be converted to H + transport knowing the stoichiometry of ATP consumption to H + transport. The P-type H + transporters found in other acidophilic algae (Ohta et al, 1997;Weiss and Pick, 1996) . This value is slightly lower than the 10 -4 -10 -7 range found in lipid membranes at neutral pH (Deamer and Akeson, 1994), but could be an underestimate, as contractile vacuole H + extrusion employs V-type H + transporters that pump 2-3 H + ions per ATP molecule hydrolyzed (for a review, see Sze et al, 1999).…”

Section: Discussionmentioning

confidence: 99%

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Life at acidic pH imposes an increased energetic cost for a eukaryotic acidophile

Messerli

Amaral‐Zettler

Zettler

et al. 2005

Journal of Experimental Biology

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Life at acidic pH imposes an increased energetic cost for a eukaryotic acidophile

Messerli

Amaral‐Zettler

Zettler

et al. 2005

Journal of Experimental Biology

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“…We performed a proteomics analysis of the PM-enriched preparation from D. salina that was extensively characterized in our laboratory (8,16,17). To enhance the analysis specificity, we tagged surface-exposed proteins in intact cells with a membrane-impermeable biotin tag, which specifically labeled plasma membrane proteins.…”

mentioning

confidence: 99%

Salt-induced Changes in the Plasma Membrane Proteome of the Halotolerant Alga Dunaliella salina as Revealed by Blue Native Gel Electrophoresis and Nano-LC-MS/MS Analysis

Katz

Waridel

Shevchenko

et al. 2007

Molecular & Cellular Proteomics

155

144

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The halotolerant alga Dunaliella salina is a recognized model photosynthetic organism for studying plant adaptation to high salinity. The adaptation mechanisms involve major changes in the proteome composition associated with energy metabolism and carbon and iron acquisition. To clarify the molecular basis for the remarkable resistance to high salt, we performed a comprehensive proteomics analysis of the plasma membrane. Plasma membrane proteins were recognized by tagging intact cells with a membrane-impermeable biotin derivative. Proteins were resolved by two-dimensional blue native/SDS-PAGE and identified by nano-LC-MS/MS. Of 55 identified proteins, about 60% were integral membrane or membraneassociated proteins. We identified novel surface coat proteins, lipid-metabolizing enzymes, a new family of membrane proteins of unknown function, ion transporters, small GTP-binding proteins, and heat shock proteins. The abundance of 20 protein spots increased and that of two protein spots decreased under high salt. The major salt-regulated proteins were implicated in protein and membrane structure stabilization and within signal transduction pathways. The migration profiles of native protein complexes on blue native gels revealed oligomerization or co-migration of major surface-exposed proteins, which may indicate mechanisms of stabilization at high salinity. Molecular & Cellular Proteomics 6:1459 -1472, 2007.The halotolerant alga Dunaliella salina uses a unique osmoregulatory mechanism and is able to proliferate in environments with extreme salt content (1). This is achieved through remarkable changes in metabolism and ion transport brought about by up-regulation or down-regulation of multiple enzymes and membrane proteins. Elucidation of the molecular basis for this remarkable adaptation might provide tools to improve the resistance of crop plants to the progressive salinization of soils, which is already a major limitation for agricultural productivity worldwide. By characterizing the changes in the soluble subproteome of D. salina, we demonstrated previously that the alga responds to hypersaline conditions by up-regulating key enzymes in photosynthetic CO 2 fixation and in energy metabolism that divert carbon metabolism to massive synthesis of glycerol, the osmotic element in Dunaliella (2).Salinity stress destabilizes biological membranes and affects the solubility of many essential substrates and ions (3). Adaptation to salinity stress might also alter the composition and organization of the membrane proteome associated with the stabilization and enhancement of ion transporters. Early studies revealed the accumulation of two carbonic anhydrases, dCAI 1 and dCAII (4, 5), and a transferrin-like protein (6) that presumably compensated the impaired availability of bicarbonate and iron under high salt. Salt-induced changes in organellar membranes, such as the expression of ER fatty acid elongase, were also reported (7). High salinity also affects sodium transport (8), lipid organization (9, 10), and activation of PM pr...

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“…The energy demand for active proton translocation is estimated to be 1 ATP per H + transported (Briskin & Hanson 1992). The abundance of H + ATPase complexes can be controlled by the cell via pH sensing (Weiss & Pick 1996). However, Nielsen et al (2007) showed that light intensity (and hence energy supply) did not correlate with cell growth under extreme pH conditions, which indicates that elevated light input does not necessarily enhance the cell's capacity to regulate internal pH.…”

Section: Homeostasis: Effect Of External Ph Manipulationmentioning

confidence: 86%

“…At pH 7, internal pH values are similar to the external medium (Smith & Raven 1979, Beardall & Raven 1981, Gehl & Colman 1985, Sianoudis et al 1987, which suggests an energetic advantage at this pH due to low costs for homeostasis. Proton regulation can be energetically expensive (Smith & Raven 1979, Briskin & Hanson 1992 and involve regulative ATPase activity (Weiss & Pick 1996). Active proton pumping might only be em ployed by the cell during short-term regulation (Beth mann & Schönknecht 2009), while steady-state homeostasis might be facilitated mainly by anion exchange (Lew 2010) in Chlorella fusca by a K + /H + antiport system (Tromballa 1987).…”

Section: Ph-dependent Acclimation Patternsmentioning

confidence: 99%

Light acclimation and pH perturbations affect photosynthetic performance in Chlorella mass culture

Ihnken¹,

Beardall²,

Kromkamp³

et al. 2014

Aquat. Biol.

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Chlorella spp. are robust chlorophyte microalgal species frequently used in mass culture. The pH optimum for growth is close to neutrality; at this pH, theoretically little energy is required to maintain homeostasis. In the present study, we grew Chlorella fusca cells in an open, outdoor, thin-layer cascade photobioreactor (TLC), under ambient photon flux at the theoretically preferred pH (7.2), and let the culture pass the exponential growth phase. Using pH drift experiments, we show that an alkalization to pH 9 supported photosynthesis in the TLC. The increased photosynthetic activity under alkaline conditions was a pH-dependent effect, and not a dissolved inorganic carbon (DIC) concentration-or light intensity-dependent effect. Re-acidification (in one step or in increments) lowered gross oxygen production and increased non-photochemical quenching in short-term experiments. Gross oxygen production and electron transport rates in PSII were uncoupled during the pH perturbation experiments. Electron transport rates were only marginally affected by pH, whereas oxygen production rates decreased with acidification. Alternative electron pathways, electron donation at the plastid terminal oxidase and state-transitions are discussed as a potential explanation. Because cell material from the TLC was not operating at maximal capacity, we propose that alkalization can support photosynthesis in challenged TLC systems.

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Primary Structure and Effect of pH on the Expression of the Plasma Membrane H+-ATPase from Dunaliella acidophila and Dunaliella salina

Cited by 33 publications

References 31 publications

Life at acidic pH imposes an increased energetic cost for a eukaryotic acidophile

Life at acidic pH imposes an increased energetic cost for a eukaryotic acidophile

Salt-induced Changes in the Plasma Membrane Proteome of the Halotolerant Alga Dunaliella salina as Revealed by Blue Native Gel Electrophoresis and Nano-LC-MS/MS Analysis

Light acclimation and pH perturbations affect photosynthetic performance in Chlorella mass culture

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