Primary structure of ten galactosides formed by transglycosylation during lactose hydrolysis by Bifidobacterium bifidum

Dumortier, V.; Montreuil, Jean; Bouquelet, Stéphane

doi:10.1016/0008-6215(90)84228-m

Cited by 32 publications

(14 citation statements)

References 20 publications

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“…However, MALDI-FTICR MS analysis of GOS syrup clearly demonstrated that there were oligosaccharides with DP of up to 15. This increase in resolution of the oligosaccharides with higher DP is likely due to the superior sensitivity and capabilities of FTICR MS compared with the highperformance liquid chromatography and nuclear magnetic resonance techniques used previously for GOS analysis (5,7,20). Similar results were reported for plant-derived fructooligosaccharides when they were analyzed by MALDI-FTICR MS (17).…”

Section: Discussionsupporting

confidence: 69%

“…GOS structures are highly variable and dependent on the enzyme and conditions used during their synthesis (5,7,23). Previous work suggested that the upper limit for the DP for GOS is 8 (11).…”

Section: Discussionmentioning

confidence: 99%

“…Thus, the basic structure of GOS includes a lactose core at the reducing end, which is typically elongated with up to six galactose residues. Structural diversity in GOS preparations is dependent on the enzyme used in the transgalactosylation reaction and the experimental conditions used, such as pH and temperature (5).…”

mentioning

confidence: 99%

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Glycoprofiling Bifidobacterial Consumption of Galacto-Oligosaccharides by Mass Spectrometry Reveals Strain-Specific, Preferential Consumption of Glycans

Barboza¹,

Sela

Pirim³

et al. 2009

Appl Environ Microbiol

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Galacto-oligosaccharides (GOS) are versatile food ingredients that possess prebiotic properties. However, at present there is a lack of precise analytical methods to demonstrate specific GOS consumption by bifidobacteria. To better understand the role of GOS as prebiotics, purified GOS (pGOS) without disaccharides and monosaccharides was prepared and used in bacterial fermentation experiments. Growth curves showed that all bifidobacteria assayed utilized and grew on pGOS preparations. We used a novel mass spectrometry approach involving matrix-assisted laser desorption ionization-Fourier transform ion cyclotron resonance (MALDI-FTICR) to determine the composition of oligosaccharides in GOS syrup preparations. MALDI-FTICR analysis of spent fermentation media demonstrated that there was preferential consumption of selected pGOS species by different bifidobacteria. The approach described here demonstrates that MALDI-FTICR is a rapid-throughput tool for comprehensive profiling of oligosaccharides in GOS mixtures. In addition, the selective consumption of certain GOS species by different bifidobacteria suggests a means for targeting prebiotics to enrich select bifidobacterial species.

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Section: Discussionsupporting

confidence: 69%

Section: Discussionmentioning

confidence: 99%

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Glycoprofiling Bifidobacterial Consumption of Galacto-Oligosaccharides by Mass Spectrometry Reveals Strain-Specific, Preferential Consumption of Glycans

Barboza¹,

Sela

Pirim³

et al. 2009

Appl Environ Microbiol

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“…The trisaccharide produced by ␤-galactosidase from B. bifidum was also identified as 3Ј-galactosyl-lactose (11). However, 4Ј-galactosyl-lactose and 6Ј-galactosyl-lactose were reported to be preferentially formed during the transgalactosylation reactions (1,39,40,62), and several ␤-galactosidases were found to produce more than one type of GaOS (1,57,62).…”

Section: Discussionmentioning

confidence: 99%

Molecular and Biochemical Analysis of Two β-Galactosidases fromBifidobacterium infantisHL96

Hung

Xia

et al. 2001

Appl Environ Microbiol

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Two genes encoding ␤-galactosidase isoenzymes, ␤-galI and ␤-galIII, from Bifidobacterium infantis HL96 were revealed on 3.6-and 2.4-kb DNA fragments, respectively, by nucleotide sequence analysis of the two fragments. ␤-galI (3,069 bp) encodes a 1,022-amino-acid (aa) polypeptide with a predicted molecular mass of 113 kDa. A putative ribosome binding site and a promoter sequence were recognized at the 5 flanking region of ␤-galI.

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“…The structures of disaccharides and trisaccharides formed during the hydrolysis with [3- 14) Streptococcus thermophilus,ll) and Bifidobacterium bifidum 16 ) were analyzed. From these reports, it can be concluded that the structures of oligo saccharides formed by transglycosylation reaction differ with the reaction conditions and the source of enzyme.…”

Section: Discussionmentioning

confidence: 99%

Formation of Oligosaccharides from Lactose byBacillus circulans β-Galactosidase

Yanahira

Kobayashi

Suguri

et al. 1995

Bioscience, Biotechnology, and Biochemistry

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Primary structure of ten galactosides formed by transglycosylation during lactose hydrolysis by Bifidobacterium bifidum

Cited by 32 publications

References 20 publications

Glycoprofiling Bifidobacterial Consumption of Galacto-Oligosaccharides by Mass Spectrometry Reveals Strain-Specific, Preferential Consumption of Glycans

Glycoprofiling Bifidobacterial Consumption of Galacto-Oligosaccharides by Mass Spectrometry Reveals Strain-Specific, Preferential Consumption of Glycans

Molecular and Biochemical Analysis of Two β-Galactosidases fromBifidobacterium infantisHL96

Formation of Oligosaccharides from Lactose byBacillus circulans β-Galactosidase

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