Primary style protein expression in the self-incompatible/compatible apricot by the 2D-DIGE technique

Cao, Xiaoyan; Feng, Jianxiang; Wang, Dajiang; Sun, Jie; Lu, Xin-Jiang; Liu, Huaifeng

doi:10.1016/j.gene.2012.04.077

Cited by 11 publications

(13 citation statements)

References 26 publications

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“…A previous study supports this hypothesis, showing that aconitase and MDH are related to the SI reaction in apricot (Cao et al . ).…”

Section: Discussionmentioning

confidence: 97%

“…This is supported by Cao et al . (), who showed that altered levels of METS affect pollen sterility during pollen recognition and germination.…”

Section: Discussionmentioning

confidence: 99%

“…Cao et al . () identified several style proteins of self‐incompatible/compatible apricot using 2‐D fluorescence difference gel electrophoresis (2D‐DIGE), and these differentially expressed proteins were mainly involved in cytoskeleton assembly and metabolism.…”

Section: Introductionmentioning

confidence: 99%

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Identification of differentially accumulating pistil proteins associated with self‐incompatibility of non‐heading Chinese cabbage

Wang

Peng

et al. 2013

Plant Biol J

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Non-heading Chinese cabbage (Brassica campestris L. ssp. chinensis Makino), an important vegetable crop in China, exhibits a typical sporophytic self-incompatibility (SI) system. To better understand the mechanism of SI response and identify potential candidate proteins involved in the SI system of this vegetable crop, the proteomic approach was taken to identify differential accumulating pistil proteins. Pistils were collected at 0 h and 2 h after self-pollination at anthesis in self-incompatible and compatible lines of non-heading Chinese cabbage, and total proteins were extracted and separated by two-dimensional gel electrophoresis (2-DE). A total of 25 protein spots that displayed differential abundance were identified by matrix-assisted laser desorption/ionisation-time of flight mass spectrometry (MALDI-TOF/TOF MS) and peptide mass fingerprinting (PMF). Among them, 22 protein spots were confidently established. The mRNA levels of the corresponding genes were detected by quantitative RT-PCR. The 22 identified protein spots are involved in energy metabolism (four), protein biosynthesis (three), photosynthesis (six), stress response and defence (five), and protein degradation (four). Among these potential candidate proteins, UDP-sugar pyrophosphorylase could be involved in sucrose degradation to influence pollen germination and growth. Glutathione S-transferases could be involved in pollen maturation, and affect pollen fertility. Senescence-associated cysteine protease, which is related to programmed cell death, could be mainly related to self pollen recognition of non-heading Chinese cabbage. The study will contribute to further investigations of molecular mechanism of sporophytic SI in Brassicaceae.

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“…A previous study supports this hypothesis, showing that aconitase and MDH are related to the SI reaction in apricot (Cao et al . ).…”

Section: Discussionmentioning

confidence: 97%

“…This is supported by Cao et al . (), who showed that altered levels of METS affect pollen sterility during pollen recognition and germination.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Identification of differentially accumulating pistil proteins associated with self‐incompatibility of non‐heading Chinese cabbage

Wang

Peng

et al. 2013

Plant Biol J

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“…We found a hypothetical gene interaction network that consisted of PAP2, CPN10, MDH, glutathione transferase, PYR6, and MS, which implied that energy metabolism and stress response would be involved in self-pollen recognition and rejection (Poulter et al, 2011;Cao et al, 2012). Among them, CPN10, glutathione transferase, PYR6, and MS were upregulated in self-incompatible plants after self-pollination, while PAP2 and MDH were downregulated.…”

Section: Discussionmentioning

confidence: 85%

“…Samuel et al (2011) indicated that enolase, tubulin alpha-2/alpha-4 chain, and ribulose-1,5-bisphosphate carboxylase oxygenase (RUBISCO)-large subunit were downregulated after incompatible pollination by two-dimensional gel electrophoresis (2-DE), and they could be related to the SI response of Brassica napus. In addition, cysteine synthase, cytosolic malate dehydrogenase, and ubiquitin-conjugating enzyme were identified to influence SI in apricot and suggested that the SI response could be mediated by cytoskeleton assembly and metabolism processes (Cao et al, 2012). We previously determined that uridine diphosphate (UDP)-sugar pyrophosphorylase and glutathione transferases were involved in the SI response of non-heading Chinese cabbage (Brassica campestris ssp chinensis Makino) by 2-DE (Wang et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Expression analysis of self-incompatibility-associated genes in non-heading Chinese cabbage

Wang

et al. 2014

Genet. Mol. Res.

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ABSTRACT. In Brassicaceae, a self-incompatibility (SI) system mediates pollen-pistil interactions. Self-pollen could be recognized and rejected by incompatible pistils. Several components involved in the SI response have been determined, including S-locus receptor kinase (SRK), S-locus cysteine-rich protein/S-locus protein 11, and arm repeatcontaining protein 1 (ARC1). However, the components involved in the SI system of Brassicaceae are not fully understood. Here, we detected expression patterns of 24 SI-related genes in non-heading Chinese cabbage (Brassica campestris ssp chinensis Makino) after compatible and incompatible pollination, and potential interaction relationships of these genes were predicted. SRK and ARC1 transcripts increased initially 0.25 h after incompatible pollination, while kinase-associated protein phosphatase had an expression pattern that was opposite that of SRK transcripts during self-pollination. Plant U-box 8 was not required in the SI response of non-heading Chinese cabbage. Our results showed that the SI signal of non-heading Chinese cabbage could occur within 0.25 h after self-pollination. The hypothetical interaction relationships indicated that plastid-lipid-associated protein and malate dehydrogenase could be negatively regulated by chaperonin 10, glutathione transferase, cytidylate kinase/uridylate kinase, and methionine synthase by indirect interactions. Our findings could be helpful to better understand potential roles of these components in the SI system of non-heading Chinese cabbage.

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iTRAQ-based quantitative proteomic analysis of pistils and anthers from self-incompatible and self-compatible almonds with the S f haplotype

et al. 2015

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Primary style protein expression in the self-incompatible/compatible apricot by the 2D-DIGE technique

Cited by 11 publications

References 26 publications

Identification of differentially accumulating pistil proteins associated with self‐incompatibility of non‐heading Chinese cabbage

Identification of differentially accumulating pistil proteins associated with self‐incompatibility of non‐heading Chinese cabbage

Expression analysis of self-incompatibility-associated genes in non-heading Chinese cabbage

iTRAQ-based quantitative proteomic analysis of pistils and anthers from self-incompatible and self-compatible almonds with the S f haplotype

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