PrimRglo: A multiplexable quantitative real-time polymerase chain reaction system for nucleic acid detection

Lai, Richard; Fang, Liang; Pearson, Darnley; Barnett, Graeme; Whiley, David M.; Sloots, Theo P.; Barnard, Ross; Corrie, Simon R.

doi:10.1016/j.ab.2011.12.038

Cited by 7 publications

(3 citation statements)

References 11 publications

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“…Lai et al ( 2012 ) developed a novel hybridization primers-based chemistry, the PrimRglo probe system, by employing a reverse primer, PrimRglo forward primer, PrimRglo forward reporter and PrimRglo reverse quencher (Fig. 6 c).…”

Section: Introductionmentioning

confidence: 99%

Trends and advances in food analysis by real-time polymerase chain reaction

et al. 2016

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Analyses to ensure food safety and quality are more relevant now because of rapid changes in the quantity, diversity and mobility of food. Food-contamination must be determined to maintain health and up-hold laws, as well as for ethical and cultural concerns. Real-time polymerase chain reaction (RT-PCR), a rapid and inexpensive quantitative method to detect the presence of targeted DNA-segments in samples, helps in determining both accidental and intentional adulterations of foods by biological contaminants. This review presents recent developments in theory, techniques, and applications of RT-PCR in food analyses, RT-PCR addresses the limitations of traditional food analyses in terms of sensitivity, range of analytes, multiplexing ability, cost, time, and pointof-care applications. A range of targets, including species of plants or animals which are used as food ingredients, food-borne bacteria or viruses, genetically modified organisms, and allergens, even in highly processed foods can be identified by RT-PCR, even at very low concentrations. Microfluidic RT-PCR eliminates the separate sample-processing step to create opportunities for point-of-care analyses. We also cover the challenges related to using RT-PCR for food analyses, such as the need to further improve sample handling.

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Section: Introductionmentioning

confidence: 99%

Trends and advances in food analysis by real-time polymerase chain reaction

et al. 2016

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“…For this purpose, many different types of nanoparticles have been used, including silica (Wu et al 2008), carbon (Fang et al 2012), and gold (Liu et al 2013). More recently, superparamagnetic iron oxide nanoparticles (SPIONs) have attracted interest since they can improve the visualization of different tissues in magnetic resonance imaging (MRI), a noninvasive method for three-dimensional imaging of tissues (Wang et al 2001).…”

Section: Nanoparticles For Tumor Imagingmentioning

confidence: 99%

“…A multiplex approach also benefits SNP genotyping (Iannone et al 2003) and can be used as a pathogen discovery tool, exemplified by the virus discovery work by . However, newer approaches, such as the PrimRglo method (Lai et al 2012), represent a push towards much greater "multiplexing" in nucleic acid detection.…”

Section: The Need For Multiplex Technologiesmentioning

confidence: 99%

RNA and DNA Diagnostics

Erdmann¹,

Jurga²,

Barciszewski³

2015

RNA Technologies

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A New, Multiplex, Quantitative Real-Time Polymerase Chain Reaction System for Nucleic Acid Detection and Quantification

Fang¹,

Arora²,

Zhang³

et al. 2013

Methods in Molecular Biology

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Quantitative real-time polymerase chain reaction (qPCR) has emerged as a powerful investigative and diagnostic tool with potential to generate accurate and reproducible results. qPCR can be designed to fulfil the four key aspects required for the detection of nucleic acids: simplicity, speed, sensitivity, and specificity. This chapter reports the development of a novel real-time multiplex quantitative PCR technology, dubbed PrimRglo™, with a potential for high-degree multiplexing. It combines the capacity to simultaneously detect many viruses, bacteria, or nucleic acids, in a single reaction tube, with the ability to quantitate viral or bacterial load. The system utilizes oligonucleotide-tagged PCR primers, along with complementary fluorophore-labelled and quencher-labelled oligonucleotides. The analytic sensitivity of PrimRglo technology was compared with the widely used Taqman(®) and SYBR green detection systems.

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PrimRglo: A multiplexable quantitative real-time polymerase chain reaction system for nucleic acid detection

Cited by 7 publications

References 11 publications

Trends and advances in food analysis by real-time polymerase chain reaction

Trends and advances in food analysis by real-time polymerase chain reaction

RNA and DNA Diagnostics

A New, Multiplex, Quantitative Real-Time Polymerase Chain Reaction System for Nucleic Acid Detection and Quantification

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