Printing biohybrid materials for bioelectronic cardio-3D-cellular constructs

Sanjuán-Alberte, Paola; Whitehead, Charlie; Jones, Joshua N.; Silva, João Carlos; Carter, Nathan; Kellaway, Simon; Hague, Richard; Cabral, Joaquim M. S.; Ferreira, Frederico Castelo; White, Lisa J.; Rawson, Frankie J.

doi:10.1016/j.isci.2022.104552

Cited by 10 publications

(3 citation statements)

References 58 publications

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“…CNTs include single-wall carbon nanotubes (SWCNTs) and multi-wall carbon nanotubes (MWCNTs). Sanjuan-Alberte et al [ 119 ] combined the conductive properties of MWCNTs with the excellent biochemical properties of dECM for the first time. The results after applying certain electrical stimuli to the scaffold show that the combination of conductive material with external electrical stimuli can drive contractile behavior similar to physiological conditions.…”

Section: Nanocomposite Hydrogel Bioinkmentioning

confidence: 99%

Hydrogels for 3D bioprinting in tissue engineering and regenerative medicine: Current progress and challenges

Fang,

Yang,

Wang

et al. 2023

IJB

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Three-dimensional (3D) bioprinting is a promising and innovative biomanufacturing technology, which can achieve precise position controlling of cells and extracellular matrix components, and further create complex and functional multi-cellular tissues or organs in a 3D environment. Bioink in the form of the cell-loaded hydrogel is most commonly used in bioprinting, and it is vital to the process of bioprinting. The bionic scaffold should possess suitable mechanical strength, biocompatibility, cell proliferation, survival, and other biological characteristics. The disadvantages of natural polymer hydrogel materials include poor mechanical properties as well as low printing performance and shape fidelity. Over the past years, a series of synthetic, modified, and nanocomposite hydrogels have been developed, which can interact through physical interactions, chemical covalent bond crosslinking, and bioconjugation reactions to change the characteristics to satisfy the requirements. In this review, a comprehensive summary is provided on recent research regarding the unique properties of hydrogel bioinks for bioprinting, with optimized methods and technologies highlighted, which have both high-value research significance and potential clinical applications. A critical analysis of the strengths and weaknesses of each hydrogel-based biomaterial ink is presented at the beginning or end of each section, alongside the latest improvement strategies employed by current researchers to address their respective shortcomings. Furthermore, we propose potential repair sites for each hydrogel-based ink based on their distinctive repair features, while reflecting on current research limitations. Finally, we synthesize and analyze expert opinions on the future of these hydrogel-based bioinks in the broader context of tissue engineering and regenerative medicine, offering valuable insights for future investigations.

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Section: Nanocomposite Hydrogel Bioinkmentioning

confidence: 99%

Hydrogels for 3D bioprinting in tissue engineering and regenerative medicine: Current progress and challenges

Fang,

Yang,

Wang

et al. 2023

IJB

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“…There are numerous promising 3D printing-based living biohybrid systems that offer unique advantages. For instance, bioelectronic cardio-3D-cellular constructs use decellularized materials as ECM [102] ; complex biological structure printing through the freeform reversible embedding of suspended hydrogels [103] ; nanocellulose-based bioink printing of human chondrocytes for cartilage tissue engineering applications [104] ; etc. [105,106] With the development of many active-based cell manipulation technologies, such as optical and acoustic force-based cell patterning, highresolution cell assembly for tissue engineering and biohybrid applications has also raised significant interest.…”

Section: D Bioprinting Of Living Cells In Hydrogelsmentioning

confidence: 99%

Living cell‐laden hydrogels: Unleashing the future of responsive biohybrid systems

Hu,

Lei,

et al. 2023

Responsive Materials

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Responsive biohybrid systems have the potential to overcome limitations of both natural and artificial machines in terms of efficiency, accuracy, and functionality. As functional units, living cells act as bricks for building biohybrid machines, where the extracellular matrix mimics hydrogels to act as ideal biological concrete. Combining living cells with hydrogels offers unique advantages in simulating human tissues or organs, which unleashes the future of biohybrid systems, and thus has attracted extensive attention. Herein, recent progress in cell‐laden hydrogel‐based responsive biohybrid systems is summarized to provide a basic understanding of how these systems are built from the bottom up and how to achieve the complex functions. This review focuses on advanced manufacturing technologies for responsive biohybrid systems, including laden cells in hydrogel matrices, three‐dimensional bioprinting, and microfluidic manufacturing. Subsequently, the innovative applications of these works, including actuators, sensors, and engineered functional materials, are presented, along with different triggering mechanisms that are highlighted. Finally, the current challenges and future opportunities in the field are addressed. This review provides a unique perspective and is hoped to inspire fields such as biohybrid technologies, soft robots, and tissue engineering.

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“…Additionally, our group has recently shown the excellent biocompatibility of a conductive bioink composed of decellularized ECM (dECM) hydrogels incorporating multi-walled CNTs (MWCNTs). The composite hydrogels were shown to improve the contractile behavior of human iPSCs-derived cardiomyocytes [ 108 ]. However, according to other studies, carbon nanotubes can exhibit varying levels of cytotoxicity, which are dependent on their purity, shape, size, and functionalization.…”

Section: Conductive Materials For Tissue Engineeringmentioning

confidence: 99%

Electrically Conductive Hydrogels for Articular Cartilage Tissue Engineering

Miguel

Barbosa

Ferreira

et al. 2022

Gels

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Articular cartilage is a highly specialized tissue found in diarthrodial joints, which is crucial for healthy articular motion. Despite its importance, articular cartilage has limited regenerative capacities, and the degeneration of this tissue is a leading cause of disability worldwide, with hundreds of millions of people affected. As current treatment options for cartilage degeneration remain ineffective, tissue engineering has emerged as an exciting approach to create cartilage substitutes. In particular, hydrogels seem to be suitable candidates for this purpose due to their biocompatibility and high customizability, being able to be tailored to fit the biophysical properties of native cartilage. Furthermore, these hydrogel matrices can be combined with conductive materials in order to simulate the natural electrochemical properties of articular cartilage. In this review, we highlight the most common conductive materials combined with hydrogels and their diverse applications, and then present the current state of research on the development of electrically conductive hydrogels for cartilage tissue engineering. Finally, the main challenges and future perspectives for the application of electrically conductive hydrogels on articular cartilage repair strategies are also discussed.

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Printing biohybrid materials for bioelectronic cardio-3D-cellular constructs

Cited by 10 publications

References 58 publications

Hydrogels for 3D bioprinting in tissue engineering and regenerative medicine: Current progress and challenges

Hydrogels for 3D bioprinting in tissue engineering and regenerative medicine: Current progress and challenges

Living cell‐laden hydrogels: Unleashing the future of responsive biohybrid systems

Electrically Conductive Hydrogels for Articular Cartilage Tissue Engineering

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