Prion protein is associated with a worse prognosis of head and neck squamous cell carcinoma

Santos, Emanuele; Fraga, Carlos Alberto de Carvalho; Xavier, Alessandra Rejane Ericsson de Oliveira; Xavier, Mauro Aparecido de Souza; Souza, Marcela Gonçalves de; Jesus, Sabrina Ferreira de; Paula, Alfredo Maurício Batista de; Farias, Lucyana Conceição; Santos, Sérgio Henrique Sousa; Santos, Tiago G.; Beraldo, Flávio H.; Guimarães, André Luiz Sena

doi:10.1111/jop.13188

Cited by 13 publications

(16 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“… Stewart et al (2019) , shown that the expression level of TP63, PSAT1, and TFRC may correlated with the function of oxidation-reduction and glutathione in squamous cell lung cancer. Santos et al (2021) , found that the expression level of PRNP may significantly correlated with lymph node metastasis progression and worse prognosis for patients with head and neck squamous cell carcinoma. López-Cortés et al (2020) , demonstrated that the protein of PRNP, S100A9, DDA1, TXN, RPS27, S100A14, S100A7, MAPK1, AGR3 and NDUFA13 were considered as the best ranked metastasis driver proteins.…”

Section: Discussionmentioning

confidence: 98%

Single-cell transcriptomics uncover the key ferroptosis regulators contribute to cancer progression in head and neck squamous cell carcinoma

Liu

Tang

et al. 2022

Front. Mol. Biosci.

View full text Add to dashboard Cite

The mechanism underlying the association between the development of head and neck squamous cell carcinoma (HNSCC) and ferroptosis is unclear. We analyzed the transcriptomes of 5902 single cells from a single-cell RNA-sequencing (scRNA-seq) dataset. They then aggregate into B cells, epithelial cells, fibroblasts, germ cells, mesenchymal cells, cancer stem cells, stem cells, T cells and endometrial cells, respectively. Our study shows that multiple pathways are significantly enriched in HNSCC development including extracellular matrix structural components, humoral immune responses, and muscle contraction. Differentially expressed genes analysis in Pseudotime analysis, pathway and biological function indicated that there was a significant correlation in the ferroptosis pathway. Furthermore, higher ferroptosis potential index (FPI) scores were significantly associated with worse overall survival prognosis in HNSCC patients. Pseudo-temporal, survival analyses and immunohistochemistry identified multiple central genes in HNSCC development, including ACSL1, SLC39A14, TFRC, and PRNP genes, and indicated associated ferroptosis. Overall, our study detected ferroptosis-related features is closely correlated with HNSCC prognosis and development, and deserved candidates suitable for immunotherapy treatment strategies determination for HNSCC patients.

show abstract

Section: Discussionmentioning

confidence: 98%

Single-cell transcriptomics uncover the key ferroptosis regulators contribute to cancer progression in head and neck squamous cell carcinoma

Liu

Tang

et al. 2022

Front. Mol. Biosci.

View full text Add to dashboard Cite

show abstract

“…IHC staining for PrP in colorectal cancer (CRC) patients showed that patients with high PrP expression had a poorer prognosis compared to PrP-negative patients (log-rank test, p < 0.0001) [49]. In head and neck squamous cell carcinoma, increased PrP expression was detected in lymph node metastasis compared to the primary lesion [50]. In lung cancer, PrP expression was mostly negative for in situ tumors, whereas PrP was expressed by invasive adenocarcinomas [51].…”

Section: Discussionmentioning

confidence: 99%

High Expression of PRNP Predicts Poor Prognosis in Korean Patients with Gastric Cancer

Choi

Moon

Eom

et al. 2022

Cancers

View full text Add to dashboard Cite

Gastric cancer (GC) has the highest occurrence and fourth-highest mortality rate of all cancers in Korea. Although survival rates are improving with the development of diagnosis and treatment methods, the five-year survival rate for stage 4 GC in Korea remains <10%. Therefore, it is important to identify candidate prognostic factors for predicting poor prognosis. PRNP is a gene encoding the prion protein PrP, which has been noted for its role in the nervous system and is known to be upregulated in various cancers and associated with both cell proliferation and metastasis. However, the value of PRNP as a prognostic factor for Korean GC patients remains unclear. Here, we analyzed the relationship between PRNP expression and survival in three independent datasets for Korean patients with GC as well as the TCGA-STAD dataset. Survival analysis indicates that high levels of PRNP expression are associated with poor overall survival of patients with GC. Gene set enrichment analysis showed that PRNP is associated with epithelial mesenchymal transition and Hedgehog signaling. In addition, proliferation of GC cell lines was inhibited after siRNA-mediated knockdown of PRNP. In conclusion, our study suggests a potential role for PRNP as a candidate prognostic factor for patients with GC.

show abstract

“…Overexpression of Syntenin‐1 in HNC was found to be significantly correlated with LNM and disease recurrence 9 . PRNP promotes the progression of LNM of HNC through up‐regulation of HSPA4, HSP90AA1, and HIF1A 10 . Nevertheless, the evidence regarding this issue is limited.…”

Section: Introductionmentioning

confidence: 99%

“…9 PRNP promotes the progression of LNM of HNC through up-regulation of HSPA4, HSP90AA1, and HIF1A. 10 Nevertheless, the evidence regarding this issue is limited.…”

mentioning

confidence: 99%

SPP1 as a key gene in the lymph node metastasis and a potential predictor of poor prognosis in head and neck carcinoma

Feng

Yuan

Sun

et al. 2022

J Oral Pathology Medicine

View full text Add to dashboard Cite

Background: Lymph node metastasis (LNM) is an important cause leading to recurrence and development of head and neck carcinoma (HNC), with the precise mechanisms unclear. Thus, we aimed to identify the key genes involved in LNM and further evaluate their expressions and roles.Methods: A cohort of HNC in the TCGA was analyzed. The study involved three phases (one screening and two validation phases). First, the differentially expressed genes regarding LNM were screened, from which a key gene was identified by a series of data mining approaches. Then, the expressions and roles of the key gene were validated in HNC through bioinformatics. Afterward, the expression of the key gene was detected by qPCR, western blot, and Immunohistochemistry based on a cell model and a tissue microarray. Further, colony formation and transwell migration and invasion assays were used to evaluate the roles of the key gene.Results: SPP1 was overexpressed in HNC tissues and was identified as the key gene.Overexpression of SPP1 in HNC was correlated with advanced pathological stages and T-stage, as well as the presence of LNM, which predicted poor prognosis. The expression of SPP1 was closely associated with the infiltration of immune cells in HNC, especially M2 macrophages. Lab experiments confirmed that SPP1 silence in HNC cells resulted in weakened invasive and metastatic abilities. Conclusion:This study reveals that SPP1 may be a key gene associated with LNM in HNC, raising the possibility of SPP1 as a target for HNC prevention and treatment.

show abstract

Prion protein is associated with a worse prognosis of head and neck squamous cell carcinoma

Cited by 13 publications

References 33 publications

Single-cell transcriptomics uncover the key ferroptosis regulators contribute to cancer progression in head and neck squamous cell carcinoma

Single-cell transcriptomics uncover the key ferroptosis regulators contribute to cancer progression in head and neck squamous cell carcinoma

High Expression of PRNP Predicts Poor Prognosis in Korean Patients with Gastric Cancer

SPP1 as a key gene in the lymph node metastasis and a potential predictor of poor prognosis in head and neck carcinoma

Contact Info

Product

Resources

About