Pristane induced effects on chromatin of rat lymphoid cells

Garrett, Lenora R.; Cuchens, Marvin A.

doi:10.1002/jcb.240450314

Cited by 11 publications

(7 citation statements)

References 31 publications

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“…Therefore, it seemed reasonable that divaleiit cations inside the intact cells were responsible for this effect, taking into account a well-recognized role of these cations in chromatin condensation ( 1,13,29). Addition of MgCl, (as well of CaCI,) in the range of [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20] mM to cell suspensions in PBS (with 0.7 mM EDTA) before treatment with detergent did result in some condensation (data not shown). However, it turned out that addition of these ions also resulted in lowering the pH by 0.2-0.3 units.…”

Section: Resultsmentioning

confidence: 99%

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Cell membrane‐dependent chromatin condensation

Vinogradov

1995

Cytometry

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It is shown by means of flow cytometry that during several seconds after cell membrane damage by a non-ionic detergent in physiologically relevant buffer solution, the chromatin of mouse thymocyte nuclei undergoes a drastic decondensation, which is revealed by a sharp increase of binding of DNAspecific fluorochromes (olivomycin or propidium iodide) and of DNA accessibility to DNAse I digestion. A similar change is observed in dead cells. Roughly half of this decondensation can be prevented by lowering the pH of the outside medium to the level known to be inside the cells; the other half remains thus far unexplained (divalent cations and the difference between small anion species seem not to be involved). The approach is based on a novel observation that fixation by formaldehyde conserves chromatin structure before the action of detergent. Flow cytometric assay is proposed for monitoring these condensation/decondensation events in media of different composition. In addition, a new approach to viable/dead cell determination, which has the advantage of immediately fixing the cell state and preserving it for a reasonably long time, is proposed. 0 1995 wiley-Liss, IIIC.

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Section: Resultsmentioning

confidence: 99%

“…The mouse thymocytes were fixed before or after treatment with 0.01% Triton X-100 (pH 7.6), washed thrice, mixed together, resuspended in PBS (at a concentration of 1.0 X 10" cells/ml), supplemented with 0.2% Triton X-100 and 10 …”

Section: Fluorochrome Accessibility Assaymentioning

confidence: 99%

Cell membrane‐dependent chromatin condensation

Vinogradov

1995

Cytometry

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“…When nuclear extracts were prepared from P+ cells that were treated with 10 pg/mL or 100 pg/mL pristane, two radioactive bands were detected after cross-linking, suggesting that two proradioactive probe; lane D, 5 pg of nuclear extract prepared from P+ cells treated with DMSO for 1 h and incubated with 20 ng of unlabeled CRE probe for 20 min at 4'C before the addition of radioactive probe; lane T, 5 pg nuclear extract prepared from P+ cells treated with TPA (1 ng/mL for 1 h) and incubated with 20 ng of unlabeled CRE probe for 20 min at 4'C before the addition of radioactive probe; lane U, reaction conducted with nuclear extract from P-cells and without DMSO; lane N, reaction conducted without nuclear extract. Gel electrophoresis was performed for 4 h as described in Materials and Methods.…”

Section: Uv Cross-linking Of Proteins T O a Crementioning

confidence: 99%

“…For example, pristane slows the growth of trout (1 61, causes arthritis in mice [17], depresses the murine immune system [lo], and increases production of growth factors [ 181. At the cellular level, pristane alters the fluidity of membranes [19] and can influence the conformation of chromatin [20].…”

Section: Introductionmentioning

confidence: 99%

Analysis of transcriptional activation of a cyclic AMP response element by 2,6,10,14‐tetramethylpentadecane (pristane) in JB6 mouse epidermal cells

Bańbura

Ackland-Berglund

Lee

et al. 1994

Molecular Carcinogenesis

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Pristane is a naturally occurring isoprenoid that is believed to be derived from the phytyl moiety of chlorophyll. Thus, it is not surprising that pristane is present in many common fruits and vegetables. Furthermore, pristane can be detected in the tissues of fish and mammals. In animal models using rodents, pristane can function as a potent tumor promoter. At the molecular level, pristane can induce changes in the plasma membrane, alter the conformation of chromatin, and selectively activate gene expression. Addition of pristane to a mouse epidermal cell line (JB6 P+) allows these cells to grow in an anchorage-independent manner. In contrast, JB6 P-cells are not transformed by pristane. Our study was undertaken to correlate transformation of P+ cells with changes induced by pristane. Transcriptional activation of a cyclic AMP response element (CRE) was induced by pristane in P+ and P-cells. Point mutations in the CRE abolished activation by pristane, thus indicating that an intact CRE was necessary for pristane activation. In P+ cells, pristane repressed phosphodiesterase activity. However, protein kinase A was activated by pristane in P+ and P-cells. Taken together, these results indicated pristane induced novel changes in P+ cells that in turn may facilitate neoplastic transformation.

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“…At the cellular level, pristane alters the fluidity of membranes [14] and can influence conformation of chromatin [15]. However, little is known concerning the uptake, metabolism, or subcellular localization of pristane.…”

Section: Introductionmentioning

confidence: 99%

The tumor promoter pristane activates transcription by a cAMP dependent mechanism

1992

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Pristane is a naturally occurring isoprenoid which is believed to be derived from the phytyl moiety of chlorophyll. Thus it is not surprising that pristane is present in many common fruits or vegetables and furthermore can be detected in tissues of fish and mammals. Using the rat as an animal model, pristane can function as a potent tumor promoter. It is conceivable that pristane could play a role in the development of certain malignancies in higher mammals since it is commonly found in the diet. At the molecular level, pristane can induce changes in the plasma membrane, alter the conformation of chromatin, as well as selectively activate gene expression. This study was undertaken to identify specific transcriptional motifs which are responsive to pristane. A transcriptional promoter which contained a cAMP response element (CRE) was consistently stimulated by pristane in several mouse and primate cell lines. A promoter construct which contained a single copy of the TPA response element (TRE) was also activated by pristane but surprisingly a promoter which contained multiple copies of the TRE was not. Activation of the TRE required 10 fold higher concentrations of pristane relative to activation of the CRE. Within two hours after addition of pristane to monkey fibroblasts (CV-1) levels of cAMP were increased more than two fold relative to controls. These data indicated that pristane can increase the level of cAMP in CV-1 cells and consequently stimulate transcriptional promoters which contain a CRE.

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Pristane induced effects on chromatin of rat lymphoid cells

Cited by 11 publications

References 31 publications

Cell membrane‐dependent chromatin condensation

Cell membrane‐dependent chromatin condensation

Analysis of transcriptional activation of a cyclic AMP response element by 2,6,10,14‐tetramethylpentadecane (pristane) in JB6 mouse epidermal cells

The tumor promoter pristane activates transcription by a cAMP dependent mechanism

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