PRM-based quantitative proteomics analysis of altered HSPs expression in villi and decidua of early missed abortion patients

Abstract

Objective: In this study，we aimed to identify differentially expressed heat shock proteins (HSPs) profile in the villi and decidua from early missed abortion(EMA) patients. Methods: By using high-throughput and high-precision Parallel Reaction Monitoring (PRM)-based targeted proteomics technique, this study studied the expressions of HSPs in villi and decidua of 10 EMA patients and 10 controls. Meanwhile, the expressions of 3 HSPs in villi of another 22 EMA patients and 22 controls were verified with Western blotting and immunohistochemistry (IHC). Results:Compared with the control group, there were potential differences in the expressions of 16 HSPs and 42 polypeptides in human villi and decidua. Among them, HSP90AB1, HSPD1 and HSPA13 were down-regulated in expressions in villi of EMA patients, with a statistically significant difference, which was consistent with the verification results of Western blot and IHC. Conclusion: Using PRM- based targeted proteomics technique, this study for the first time screens and quantitatively analyzes the expression profile of HSPs in villi and decidua of patients with EMA. The significant down-regulated expressions of HSP90AB1, HSPD1 and HSPA13 are discovered to have a potentially intimate association with the occurrence of EMA. Findings in our study may provide novel potential research targets related to HSPs for the pathogenesis, prevention and treatment of EMA.

Secretory Proteomic Responses of Endometrial Epithelial Cells to Trophoblast-Derived Extracellular Vesicles

“…Differentially expressed proteins in the JAr EV-treated RL95-2 cell secretome with a potential role in the process of embryo implantation (evidence related to the embryo implantation process, including endometrial receptivity, embryo implantation, and early embryo development, is provided). [14, [65][66][67][68][69] Orthogonal validation of the mass spectrometry data was performed on the MFGE8 protein using ELISA (Figure 2). Here, the data indicate that trophoblast-derived EVs can enrich the endometrial cell secretory proteome with known players of embryo implantation.…”